Phenolic Alkaloids from Menispermum dauricum Rhizome Protect against Brain Ischemia Injury via Regulation of GLT-1, EAAC1 and ROS Generation
Abstract
:1. Introduction
2. Results and Discussion
2.1. Effect of PAM on OGD-Reoxygenation Induced Cell Injury
2.2. Effects of PAM on OGD-Evoked Increase of Extracellular Glutamate Content
2.3. Effects of PAM on the Level of GLT-1 and EAAC1 after OGD 90 min
2.4. Effects of PAM on OGD-Reoxygenation Evoked Increase of Intracellular ROS Generation
2.5. Effects of PAM on Cerebral Infarct Area and Neurological Deficit Score
3. Experimental
3.1. Chemicals
3.2. Preparation of PAM
3.3. Primary Rat Cortical Cultures and Oxygen-Glucose Deprivation Followed by Reoxygenation
3.4. MTT Reduction Test and LDH Assay
3.5. Glutamate Assay in the Culture Medium
3.6. Western Blot Analysis of GLT-1 and EAAC1
3.7. Intracellular ROS Measurement
3.8. Animals
3.9. Cerebral Ischemia-Reperfusion Model in Rat and Drug Administration
3.10. Neurological Deficit Evaluation and Infarct Size Measurement
3.11. Statistical Analysis
4. Conclusions
Acknowledgements
- Samples Availability: Samples of the compound PAM is available from the authors.
References and Notes
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Zhao, B.; Chen, Y.; Sun, X.; Zhou, M.; Ding, J.; Zhan, J.-J.; Guo, L.-J. Phenolic Alkaloids from Menispermum dauricum Rhizome Protect against Brain Ischemia Injury via Regulation of GLT-1, EAAC1 and ROS Generation. Molecules 2012, 17, 2725-2737. https://doi.org/10.3390/molecules17032725
Zhao B, Chen Y, Sun X, Zhou M, Ding J, Zhan J-J, Guo L-J. Phenolic Alkaloids from Menispermum dauricum Rhizome Protect against Brain Ischemia Injury via Regulation of GLT-1, EAAC1 and ROS Generation. Molecules. 2012; 17(3):2725-2737. https://doi.org/10.3390/molecules17032725
Chicago/Turabian StyleZhao, Bo, Yang Chen, Xi Sun, Mei Zhou, Jie Ding, Jin-Jin Zhan, and Lian-Jun Guo. 2012. "Phenolic Alkaloids from Menispermum dauricum Rhizome Protect against Brain Ischemia Injury via Regulation of GLT-1, EAAC1 and ROS Generation" Molecules 17, no. 3: 2725-2737. https://doi.org/10.3390/molecules17032725