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Open AccessCommunication

Large-Scale Separation of Alkaloids from Corydalis bungeana Turcz. by pH-Zone-Refining Counter-Current Chromatography

1
Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan 250014, Shandong, China
2
Institute of Chinese Material Medical, China Academy of Chinese Medical Sciences, 16 Dongzhimennei Street, Beijing 100700, China
3
China National institute of Standardization, 4 Zhichun Road, Beijing 100088, China
*
Author to whom correspondence should be addressed.
Molecules 2012, 17(12), 14968-14974; https://doi.org/10.3390/molecules171214968
Received: 31 October 2012 / Revised: 27 November 2012 / Accepted: 11 December 2012 / Published: 17 December 2012
pH-Zone-refining counter-current chromatography (pH-zone-refining CCC) was successfully applied for the large-scale separation of alkaloids from Corydalis bungeana. The crude extract was separated by a two-phase solvent system composed of petroleum ether–ethyl acetate–methanol–water (5:5:2:8, v/v) where triethylamine (10 mM) was added to the upper organic stationary phase as a retainer and hydrochloric acid (5 mM) to the aqueous mobile phase as a displacer. As a result, 285 mg of protopine, 86 mg of corynoloxine, 430 mg of coryno1ine, and 115 mg of acetylcorynoline were obtained from 3.0 g of crude extract in a one-step separation. The purities of these compounds were 99.1%, 98.3%, 99.0% and 98.5%, respectively, as determined by HPLC. The chemical structures of these isolated compounds were confirmed by ESI-MS, 1H-NMR and 13C-NMR. View Full-Text
Keywords: pH-zone-refining counter-current chromatography; large-scale separation; Corydalis bungeana; alkaloids pH-zone-refining counter-current chromatography; large-scale separation; Corydalis bungeana; alkaloids
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Wang, X.; Dong, H.; Shu, X.; Zheng, Z.; Yang, B.; Huang, L. Large-Scale Separation of Alkaloids from Corydalis bungeana Turcz. by pH-Zone-Refining Counter-Current Chromatography. Molecules 2012, 17, 14968-14974.

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