Rapid and Comprehensive Evaluation of (Poly)phenolic Compounds in Pomegranate (Punica granatum L.) Juice by UHPLC-MSn
by
Pedro Mena 1, Luca Calani 2, Chiara Dall'Asta 3, Gianni Galaverna 3
, Cristina García-Viguera 1, Renato Bruni 3, Alan Crozier 4 and Daniele Del Rio 2,*
Pedro Mena 1, Luca Calani 2, Chiara Dall'Asta 3, Gianni Galaverna 3, Cristina García-Viguera 1, Renato Bruni 3, Alan Crozier 4 and Daniele Del Rio 2,*
1
Phytochemistry Laboratory, Department of Food Science and Technology, CEBAS-CSIC, Murcia 30100, Spain
2
The φ2 Laboratory of Phytochemicals in Physiology, Department of Food Science, University of Parma, Parma 43125, Italy
3
Department of Food Science, University of Parma, Parma 43125, Italy
4
North Lab., School of Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK
*
Author to whom correspondence should be addressed.
Molecules 2012, 17(12), 14821-14840; https://doi.org/10.3390/molecules171214821
Received: 16 November 2012 / Revised: 7 December 2012 / Accepted: 10 December 2012 / Published: 13 December 2012
(This article belongs to the Special Issue Methods in Polyphenol Analysis)
The comprehensive identification of phenolic compounds in food and beverages is a crucial starting point for assessing their biological, nutritional, and technological properties. Pomegranate (Punica granatum L.) has been described as a rich source of (poly)phenolic components, with a broad array of different structures (phenolic acids, flavonoids, and hydrolyzable tannins) and a quick, high throughput, and accurate screening of its complete profile is still lacking. In the present work, a method for UHPLC separation and linear ion trap mass spectrometric (MSn) characterization of pomegranate juice phenolic fraction was optimized by comparing several different analytical conditions. The best solutions for phenolic acids, anthocyanins, flavonoids, and ellagitannins have been delineated and more than 70 compounds have been identified and fully characterized in less than one hour total analysis time. Twenty-one compounds were tentatively detected for the first time in pomegranate juice. The proposed fingerprinting approach could be easily translated to other plant derived food extracts and beverages containing a wide array of phytochemical compounds.
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Keywords:
Punica granatum L.; source ionization conditions; ellagitannins; C18 and PFP columns; phytochemicals identification
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This is an open access article distributed under the Creative Commons Attribution License
MDPI and ACS Style
Mena, P.; Calani, L.; Dall'Asta, C.; Galaverna, G.; García-Viguera, C.; Bruni, R.; Crozier, A.; Del Rio, D. Rapid and Comprehensive Evaluation of (Poly)phenolic Compounds in Pomegranate (Punica granatum L.) Juice by UHPLC-MSn. Molecules 2012, 17, 14821-14840.
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