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Molecules 2012, 17(1), 740-752;

Comparison of Polysaccharides from Two Species of Ganoderma

1,2, 1,2,* , 1,2, 3, 3 and 1,2,3,*
State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, Macao SAR 999078, China
Institute of Chinese Medical Sciences, University of Macau, Macao SAR 999078, China
Jiangsu Key Laboratory for TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210029, China
Authors to whom correspondence should be addressed.
Received: 20 December 2011 / Revised: 9 January 2012 / Accepted: 10 January 2012 / Published: 13 January 2012
(This article belongs to the Special Issue Natural Polysaccharides: Chemistry, Bioactivity and Analysis)
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Ganoderma lucidum and Ganoderma sinense, known as Lingzhi in Chinese, are commonly used Chinese medicines with excellent beneficial health effects. Triterpenes and polysaccharides are usually considered as their main active components. However, the content of triterpenes differs significantly between the two species of Ganoderma. To date, a careful comparison of polysaccharides from the two species of Ganoderma has not been performed. In this study, polysaccharides from fruiting bodies of two species of Lingzhi collected from different regions of China were analyzed and compared based on HPSEC-ELSD and HPSEC-MALLS-RI analyses, as well as enzymatic digestion and HPTLC of acid hydrolysates. The results indicated that both the HPSEC-ELSD profiles and the molecular weights of the polysaccharides were similar. Enzymatic digestion showed that polyshaccharides from all samples of Lingzhi could be hydrolyzed by pectinase and dextranase. HPTLC profiles of their TFA hydrolysates colored with different reagents and their monosaccharides composition were also similar. View Full-Text
Keywords: Lingzhi; polysaccharides; HPSEC-ELSD; HPSEC-MALLS-RI; enzymatic digestion; HPTLC Lingzhi; polysaccharides; HPSEC-ELSD; HPSEC-MALLS-RI; enzymatic digestion; HPTLC

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Xie, J.; Zhao, J.; Hu, D.-J.; Duan, J.-A.; Tang, Y.-P.; Li, S.-P. Comparison of Polysaccharides from Two Species of Ganoderma. Molecules 2012, 17, 740-752.

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