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Kazinol Q from Broussonetia kazinoki Enhances Cell Death Induced by Cu(ll) through Increased Reactive Oxygen Species

Department of Life Science, National Taitung University, Taitung 950, Taiwan
Department of Life Science, Tzu-Chi University, Hualien 970, Taiwan
Author to whom correspondence should be addressed.
Molecules 2011, 16(4), 3212-3221;
Received: 21 February 2011 / Revised: 8 April 2011 / Accepted: 12 April 2011 / Published: 15 April 2011
(This article belongs to the Section Natural Products Chemistry)
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The ability of the flavan kazinol Q (KQ) to induce DNA breakage in the presence of Cu(II) was examined by agarose gel electrophoresis using supercoiled plasmid DNA. In KQ-mediated DNA breakage reaction, the involvement of reactive oxygen species (ROS), H2O2 and O2 - was established by the inhibition of DNA breakage by catalase and revealed DNA breakage by superoxide dismutase (SOD). The cell viability of gastric carcinoma SCM-1 cells treated with various concentrations of KQ was significantly decreased by cotreatment with Cu(II). Treatment of SCM-1 cells with 300 μM Cu(II) enhanced the necrosis induced by 100 μM KQ. Treatment of SCM-1 cells with 100 mM KQ in the presence of 300 mM Cu(II) increased the generation of H2O2. Taken together, the above finding suggested that KQ cotreatment with Cu(II) produced increased amounts of H2O2, thus enhancing subsequent cell death due to necrosis. View Full-Text
Keywords: kazinol Q; copper; DNA breakage; ROS kazinol Q; copper; DNA breakage; ROS

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Wei, B.-L.; Chen, Y.-C.; Hsu, H.-Y. Kazinol Q from Broussonetia kazinoki Enhances Cell Death Induced by Cu(ll) through Increased Reactive Oxygen Species. Molecules 2011, 16, 3212-3221.

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