On the Reducible Character of Haldane-Radić Enzyme Kinetics to Conventional and Logistic Michaelis-Menten Models
Laboratory of Computational and Structural Physical Chemistry, Chemistry Department, West University of Timişoara, Pestalozzi Street No.16, Timisoara, RO-300115, Romania
Molecules 2011, 16(4), 3128-3145; https://doi.org/10.3390/molecules16043128
Received: 11 March 2011 / Revised: 13 April 2011 / Accepted: 13 April 2011 / Published: 13 April 2011
(This article belongs to the Special Issue Enzyme-Catalyzed Reactions)
The conceptual and practical issues regarding the reduction of the Haldane-Radić enzymic mechanism, specific for cholinesterase kinetics, to the consecrated or logistically modified Michaelis-Menten kinetics, specific for some mutant enzymes, are here clarified as due to the limited initial substrate concentration, through detailed initial rate and progress curve analysis, even when other classical conditions for such equivalence are not entirely fulfilled.
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Keywords:
W-Lambert equation; logistic transformation; cholinesterases; fitting curves; kinetic parameters
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This is an open access article distributed under the Creative Commons Attribution License
MDPI and ACS Style
Putz, M.V. On the Reducible Character of Haldane-Radić Enzyme Kinetics to Conventional and Logistic Michaelis-Menten Models. Molecules 2011, 16, 3128-3145.
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