Triterpene Composition and Bioactivities of Centella asiatica
Abstract
:1. Introduction
2. Results and Discussion
3. Experimental
3.1. Plant materials
3.2. Chemicals and materials
3.3. Preparation of ethanolic-aqueous extraction
3.4. High performance liquid chromatography (HPLC) analysis
3.5. Protein assay
3.6. Culture of human dermal fibroblasts preparation
3.7. Assay of Collagen by sirius red staining method
3.8. Lipolytic activity
3.9. Measurement of the free radical scavenging activity
3.10. UV protection activity
3.11. Statistical analysis
4. Conclusions
Acknowledgements
References
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Sample Availability: Samples of the herbarium specimen of Centella (FRI50032) is available from the authors. |
Types of triterpenes | Retention time (min) | Concentration of triterpenes in Centella extract (mg/mL) |
---|---|---|
Madecassoside | 6.313 | 3.10 ± 4.58a |
Asiaticoside | 7.997 | 1.97 ± 2.65b |
Madecassic acid | 12.199 | 0.55 ± 2.29c |
Asiatic acid | 13.573 | 0.55 ± 0.89c |
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Hashim, P.; Sidek, H.; Helan, M.H.M.; Sabery, A.; Palanisamy, U.D.; Ilham, M. Triterpene Composition and Bioactivities of Centella asiatica. Molecules 2011, 16, 1310-1322. https://doi.org/10.3390/molecules16021310
Hashim P, Sidek H, Helan MHM, Sabery A, Palanisamy UD, Ilham M. Triterpene Composition and Bioactivities of Centella asiatica. Molecules. 2011; 16(2):1310-1322. https://doi.org/10.3390/molecules16021310
Chicago/Turabian StyleHashim, Puziah, Hamidah Sidek, Mohd Helme M. Helan, Aidawati Sabery, Uma Devi Palanisamy, and Mohd Ilham. 2011. "Triterpene Composition and Bioactivities of Centella asiatica" Molecules 16, no. 2: 1310-1322. https://doi.org/10.3390/molecules16021310
APA StyleHashim, P., Sidek, H., Helan, M. H. M., Sabery, A., Palanisamy, U. D., & Ilham, M. (2011). Triterpene Composition and Bioactivities of Centella asiatica. Molecules, 16(2), 1310-1322. https://doi.org/10.3390/molecules16021310