Effects of Lycium Barbarum Aqueous and Ethanol Extracts on High-Fat-Diet Induced Oxidative Stress in Rat Liver Tissue
Abstract
:Abbreviations
(LBAE) | aqueous extract of Lycium barbarum |
(LBEE) | ethanol extract of Lycium barbarum |
(HF) | high fat diet |
(TC) | total cholesterol |
(TG) | triglyceride |
(HDL-C) | high density lipoprotein cholesterol |
(LDL-C) | low density lipoprotein cholesterol |
(ALT) | alanine aminotransferase |
(AST) | aspartate aminotransferase |
(ALP) | alkaline phosphatase |
(GSH-Px) | glutathione peroxidase |
(MDA) | malondialdehyde |
(GSH) | glutathione |
(CAT) | catalase |
(SOD) | superoxide dismutase |
(TAOC) | total antioxidant capacity |
1. Introduction
2. Results
Group | Body weight | Liver index | |
---|---|---|---|
initial | end | ||
NC | 80.43 + 5.32 | 156.63 + 12.64 | 4.05 + 0.32 |
HF | 81.53 + 4.93 | 180.54 + 14.09 b | 4.78 + 0.26 b |
HF + simvastatin (20 mg/kg b.w.) | 79.94 + 6.05 | 161.87 + 13.28 c | 4.32 + 0.31 c |
HF + LBAE (50 mg/kg b.w.) | 80.61 + 5.37 | 172.6 + 13.02 | 4.71 + 0.29 |
HF + LBAE (100 mg/kg b.w.) | 80.09 + 4.93 | 164.07 + 14.28 c | 4.52 + 0.32 |
HF + LBEE (50 mg/kg b.w.) | 81.54 + 4.43 | 169.31 + 13.33 | 4.34 + 0.25 c |
HF + LBEE (100 mg/kg b.w.) | 79.99 + 6.03 | 157.39 + 12.18 d | 4.11 + 0.27 c |
Group | TC (mmol/L) | TG (mmol/L) | LDL-c (mmol/L) | HDL-c (mmol/L) |
---|---|---|---|---|
NC | 2.37 + 0.14 | 0.69 + 0.05 | 0.91 + 0.04 | 1.22 + 0.11 |
HF | 6.07 + 0.32 b | 1.29 + 0.16 b | 4.02 + 0.03 b | 0.89 + 0.07 b |
HF + simvastatin (20 mg/kg b.w.) | 3.93 + 0.21 d | 0.88 + 0.03 d | 2.01 + 0.13 d | 1.13 + 0.09 d |
HF + LBAE (50 mg/kg b.w.) | 5.63 + 0.29 | 1.04 + 0.08 | 3.31 + 0.24 c | 0.92 + 0.06 |
HF + LBAE (100 mg/kg b.w.) | 4.26 + 0.19 d | 0.91 + 0.06 c | 2.84 + 0.18 d | 1.01 + 0.08 c |
HF + LBEE (50 mg/kg b.w.) | 3.41 + 0.14 d | 0.91 + 0.08 d | 2.57 + 0.14 d | 1.09 + 0.05 c |
HF + LBEE (100 mg/kg b.w.) | 2.29 + 0.16 d | 0.72 + 0.05 d | 1.05 + 0.09 d | 1.2 + 0.11 d |
Group | AST (U/L) | ALP (U/L) | ALT (U/L) |
---|---|---|---|
NC | 182.6 + 10.52 | 137.42 + 10.42 | 35.86 + 1.43 |
HF | 538.11 + 32.64 b | 497.15 + 25.62 b | 333.27 + 20.64 b |
HF + simvastatin (20 mg/kg b.w.) | 273.06 + 17.59 d | 251.37 + 22.16 d | 92.15 + 5.32 d |
HF + LBAE (50 mg/kg b.w.) | 478.21 + 30.14 c | 408.66 + 26.53 c | 281.39 + 16.42 c |
HF + LBAE (100 mg/kg b.w.) | 370.29 + 24.1 d | 354.08 + 17.99 d | 217.58 + 17.03 d |
HF + LBEE (50 mg/kg b.w.) | 385.32 + 19.46 d | 341.47 + 21.57 d | 194.34 + 13.29 d |
HF + LBEE (100 mg/kg b.w.) | 227.48 + 13.82 d | 216.04 + 17.63 d | 82.14 + 5.38 d |
Group | MDA (nmol/mg Prot) | GSH (mg/g Prot) |
---|---|---|
NC | 2.74 + 0.12 | 12.48 + 1.06 |
HF | 6.92 + 0.45 b | 6.04 + 0.73 b |
HF + simvastatin (20 mg/kg b.w.) | 4.35 + 0.32 d | 11.43 + 0.97 d |
HF + LBAE (50 mg/kg b.w.) | 5.83 + 0.33 c | 8.74 + 0.77 d |
HF + LBAE (100 mg/kg b.w.) | 4.01 + 0.19 d | 9.83 + 0.81 d |
HF + LBEE (50 mg/kg b.w.) | 4.41 + 0.21 d | 9.91 + 0.85 d |
HF + LBEE (100 mg/kg b.w.) | 2.93 + 0.12 d | 13.51 + 1.24 d |
Group | SOD | CAT | GSH-Px | TAOC |
---|---|---|---|---|
(U/mg prot.) | (U/mg prot.) | (U/mg prot.) | (U/mg prot.) | |
NC | 285.1 + 17.49 | 31.73 + 1.64 | 29.07 + 1.11 | 3.08 + 0.14 |
HF | 121.7 + 10.83 b | 12.08 + 1.02 b | 11.38 + 0.94 b | 1.25 + 0.11 b |
HF + simvastatin (20 mg/kg b.w.) | 231.6 + 18.32 d | 26.37 + 1.77 d | 28.99 + 0.99 d | 2.83 + 0.2 d |
HF + LBAE (50 mg/kg b.w.) | 142.3 + 9.83 c | 20.15 + 1.48 d | 15.38 + 1.04 c | 1.52 + 0.11 c |
HF + LBAE (100 mg/kg b.w.) | 179.3 + 14.01 d | 24.64 + 1.33 d | 23.15 + 1.32 d | 2.57 + 0.15 d |
HF + LBEE (50 mg/kg b.w.) | 223.1 + 16.38 d | 25.21 + 1.09 d | 22.65 + 1.37 d | 2.62 + 0.17 d |
HF + LBEE (100 mg/kg b.w.) | 288.4 + 24.09 d | 30.75 + 1.42 d | 30.63 + 1.54 d | 3.14 + 0.27 d |
3. Discussion
4. Material and Method
4.1. Plant Material and Extraction
4.2. Animals and Dietary Treatment
Ingredients | ND | HFD |
---|---|---|
Casein | 200 | 200 |
dl-Methionine | 3 | 3 |
Corn starch | 150 | 111 |
Sucrose | 500 | 370 |
Cellulose | 50 | 50 |
Corn oil | 50 | 30 |
Lard | – | 170 |
Mineral mixture a | 35 | 42 |
Vitamin mixture b | 10 | 12 |
Choline bitartrate | 2 | 2 |
Cholesterol | – | 10 |
tert-Butylhydroquinone c | 0.01 | 0.04 |
Fat, % kJ | 11.5 | 40.0 |
Total energy, kJ/kg diet | 16,439 | 19,315 |
4.3. Histopathological Analysis
4.4. Biochemical Analysis
4.5. Statistical Analysis
5. Conclusions
Acknowledgements
References
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Cui, B.; Liu, S.; Lin, X.; Wang, J.; Li, S.; Wang, Q.; Li, S. Effects of Lycium Barbarum Aqueous and Ethanol Extracts on High-Fat-Diet Induced Oxidative Stress in Rat Liver Tissue. Molecules 2011, 16, 9116-9128. https://doi.org/10.3390/molecules16119116
Cui B, Liu S, Lin X, Wang J, Li S, Wang Q, Li S. Effects of Lycium Barbarum Aqueous and Ethanol Extracts on High-Fat-Diet Induced Oxidative Stress in Rat Liver Tissue. Molecules. 2011; 16(11):9116-9128. https://doi.org/10.3390/molecules16119116
Chicago/Turabian StyleCui, BoKang, Su Liu, XiaoJun Lin, Jun Wang, ShuHong Li, QiBo Wang, and ShengPing Li. 2011. "Effects of Lycium Barbarum Aqueous and Ethanol Extracts on High-Fat-Diet Induced Oxidative Stress in Rat Liver Tissue" Molecules 16, no. 11: 9116-9128. https://doi.org/10.3390/molecules16119116