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Molecules 2010, 15(9), 6140-6151;

Screening of Filamentous Fungi to Identify Biocatalysts for Lupeol Biotransformation

Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, 14040-903, Brazil
Núcleo de Pesquisa em Ciências Exatas e Tecnológicas, Universidade de Franca, Franca, SP, 14404-600, Brazil
Instituto de Ciências Exatas e da Terra, Universidade Federal de Mato Grosso, MT, 78060-900, Brazil
Centro de Ciências Exatas e de Tecnologia, Universidade Federal de São Carlos, SP, 13565-905, Brazil
Author to whom correspondence should be addressed.
Received: 11 August 2010 / Revised: 30 August 2010 / Accepted: 30 August 2010 / Published: 1 September 2010
(This article belongs to the Section Natural Products Chemistry)
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The goal of the study was to evaluate the ability of filamentous fungi to biotransform the pentacyclic triterpene lupeol. The microbial transformations were carried out in shake flasks in different media. Experiments were also run with control flasks. Samples of each culture were taken every 24 hours, extracted with ethyl acetate, and analyzed by GC-MS. The biotransformation of lupeol by Aspergillus ochraceus and Mucor rouxii afforded two compounds in each culture, which were detected in the cultures developed for more than seven days only in the Koch’s K1 medium. The obtained data demonstrated that A. ochraceus is a good biocatalyst to introduce double bonds in the lupeol structure, whereas M. rouxii exhibits ability to biocatalyze oxygen insertions in that pentacyclic triterpene. Mass spectrometry was demonstrated to be an efficient analytical method to select promising biocatalysts for the compound investigated in this study. The biotransformation processes were influenced by the culture medium and incubation period. The obtained results open the perspective of using A. ochraceus and M. rouxii in pentacyclic triterpene biotransformations.
Keywords: biocatalysts; biotransformation; filamentous fungi; lupeol; mass spectrometry biocatalysts; biotransformation; filamentous fungi; lupeol; mass spectrometry
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Carvalho, T.C.; Polizeli, A.M.; Turatti, I.C.C.; Severiano, M.E.; Carvalho, C.E.; Ambrósio, S.R.; Crotti, A.E.M.; Figueiredo, U.S.; Vieira, P.C.; Furtado, N.A.J.C. Screening of Filamentous Fungi to Identify Biocatalysts for Lupeol Biotransformation. Molecules 2010, 15, 6140-6151.

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