Next Article in Journal
Yields and Constituents of Essential Oil from Cones of Pinaceae spp. Natively Grown in Turkey
Previous Article in Journal
A Facile Synthesis of 3-Substituted 9H-Pyrido[3,4-b]indol-1(2H)-one Derivatives from 3-Substituted β-Carbolines
Article Menu

Export Article

Open AccessArticle
Molecules 2010, 15(8), 5742-5755; https://doi.org/10.3390/molecules15085742

Development of a Sphingosylphosphorylcholine Detection System Using RNA Aptamers

1
VALWAY Technology Center, NEC Soft, Ltd., Tokyo 136-8627, Japan
2
Biotechnology Research Group, Fundamental Research Department, Fujirebio Inc., Tokyo, 192-0031, Japan
*
Author to whom correspondence should be addressed.
Received: 27 July 2010 / Revised: 16 August 2010 / Accepted: 17 August 2010 / Published: 20 August 2010
  |  
PDF [309 KB, uploaded 18 June 2014]
  |  

Abstract

Sphingosylphosphorylcholine (SPC) is a lysosphingolipid that exerts multiple functions, including acting as a spasmogen, as a mitogenic factor for various types of cells, and sometimes as an inflammatory mediator. Currently, liquid chromatography/tandem mass spectrometry (LC/MS/MS) is used for the quantitation of SPC. However, because of the complicated procedures required it may not be cost effective, hampering its regular usage in a routine practical SPC monitoring. In this report, we have generated RNA aptamers that bind to SPC with high affinity using an in vitro selection procedure and developed an enzyme-linked aptamer assay system using the minimized SPC aptamer that can successfully distinguish SPC from the structurally related sphingosine 1-phosphate (S1P). This is the first case of the Systematic Evolution of Ligands by EXponential enrichment (SELEX) process being performed with a lysosphingolipid. The SPC aptamers would be valuable tools for the development of aptamer-based medical diagnosis and for elucidating the biological role of SPC. View Full-Text
Keywords: aptamer; enzyme-linked aptamer assay; sphingosine 1-phosphate; sphingosylphosphorylcholine; systematic evolution of ligands by exponential enrichment aptamer; enzyme-linked aptamer assay; sphingosine 1-phosphate; sphingosylphosphorylcholine; systematic evolution of ligands by exponential enrichment
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Horii, K.; Omi, K.; Yoshida, Y.; Imai, Y.; Sakai, N.; Oka, A.; Masuda, H.; Furuichi, M.; Tanimoto, T.; Waga, I. Development of a Sphingosylphosphorylcholine Detection System Using RNA Aptamers. Molecules 2010, 15, 5742-5755.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top