Identification of Major Phenolic Compounds from Nephelium lappaceum L. and Their Antioxidant Activities
Abstract
:1. Introduction
2. Results and Discussion
2.1. Isolation and identification of bioactive compounds from N. lappaceum peel
2.2. Quantification of bioactive identified components
2.3. Antioxidant activity
Compounds | Lipid peroxidation inhibition capacity | DPPH• scavenging activity | ||
---|---|---|---|---|
IC50 (µM) | Mol BHT/mol phenolics | IC50 (µM) | Mol BHT/mol phenolics | |
Geraniin | 0.38 ± 0.01 a | 186 ± 3.00 | 0.79 ± 0.05 a | 87.1 ± 5.76 |
Corilagin | 0.70 ± 0.03 b | 103 ± 3.72 | 1.42 ± 0.10 b | 48.6 ± 3.44 |
Ellagic acid | 0.94 ± 0.10 c | 76.9 ± 7.63 | 1.64 ± 0.15 b | 42.4 ± 3.85 |
Gallic acid | 1.70 ± 0.13 d | 42.2 ± 3.10 | 2.49 ± 0.11 c | 27.7 ± 1.15 |
BHT | 71.3 ± 0.86 | - | 68.8 ± 2.10 | - |
Reference | IC50 (µM) | |||
---|---|---|---|---|
gallic acid | ellagic acid | corilagin | geraniin | |
Zheng et al., 2009 [30] | 4.71 | 3.17 | ||
Latté et al., 2004 [31] | 32.9 | 2.70 | ||
Xu et al., 2007 [32] | 27.4 | 18.7 | ||
Lin et al., 2008 [24] | 1.27 (pH 7.9), 0.92 (pH 4.5) | |||
Yokozawa et al., 1999 [33] | 8.14 | 4.60 | 2.89 | 2.50 |
This work | 2.49 | 1.64 | 1.42 | 0.79 |
3. Experimental
3.1. General
3.2. Extraction, isolation and identification of bioactive compounds from N. lappaceum peel
3.3. Phenazine derivative preparation
3.4. Analytical HPLC and quantification of bioactive components
3.5. Determination of antioxidant activities
3.5.1. Linoleic peroxidation method
3.5.2. DPPH• scavenging activity
3.6. Statistical analysis
4. Conclusions
Acknowledgements
- Sample Availability: Samples of the compounds are available from the authors.
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Thitilertdecha, N.; Teerawutgulrag, A.; Kilburn, J.D.; Rakariyatham, N. Identification of Major Phenolic Compounds from Nephelium lappaceum L. and Their Antioxidant Activities. Molecules 2010, 15, 1453-1465. https://doi.org/10.3390/molecules15031453
Thitilertdecha N, Teerawutgulrag A, Kilburn JD, Rakariyatham N. Identification of Major Phenolic Compounds from Nephelium lappaceum L. and Their Antioxidant Activities. Molecules. 2010; 15(3):1453-1465. https://doi.org/10.3390/molecules15031453
Chicago/Turabian StyleThitilertdecha, Nont, Aphiwat Teerawutgulrag, Jeremy D. Kilburn, and Nuansri Rakariyatham. 2010. "Identification of Major Phenolic Compounds from Nephelium lappaceum L. and Their Antioxidant Activities" Molecules 15, no. 3: 1453-1465. https://doi.org/10.3390/molecules15031453