Antioxidant Phenolic Compounds of Dracaena Cambodiana
Abstract
:1. Introduction
2. Results and Discussion
2.1. Fractions
Concentration μg/mL | DPPH radical-scavenging capacity (%) | ABTS free radical-scavenging capacity (%) | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
PEF | EAF | BUF | AF | Vc | PEF | EAF | BUF | AF | Trolox® | |
800 | 11.90 | 29.49 | 18.22 | 12.45 | ND | 28.79 | 69.10 | 42.42 | 35.96 | ND |
400 | - | 16.02 | - | - | ND | 17.56 | 39.47 | 25.43 | 23.46 | ND |
200 | 2.93 | - | 6.04 | 7.33 | ND | 12.08 | 23.03 | 15.73 | 17.98 | 99.58 |
100 | 1.60 | 4.30 | 3.30 | 2.66 | 73.22 | 7.86 | 14.61 | 10.39 | 8.43 | 65.45 |
50 | 0.64 | 3.94 | 2.84 | 1.74 | 35.61 | 5.62 | 10.39 | 7.44 | 7.16 | 38.34 |
25 | 0.18 | 2.66 | 0.73 | 1.65 | 19.56 | 4.78 | 9.41 | 6.60 | 5.06 | 16.43 |
12.5 | ND | ND | ND | ND | 5.41 | ND | ND | ND | ND | 10.39 |
6.25 | ND | ND | ND | ND | 4.71 | ND | ND | ND | ND | 6.60 |
Test materials | DPPH EC50 | ABTS IC50 | Superoxideradical (mol α-Tocopherol/g DW) | Total phenolics (GAE g/g extract) | Total flavonoids (RE mg/g extract) |
---|---|---|---|---|---|
PEF | 5.007 | 1.510 | 0.221 ± 0.12 | 4.88 ± 1.35b | 0 |
EAF | 1.613 | 0.535 | 1.028 ±0.53 | 27.82 ± 1.02a | 65.70 ± 2.44a |
BUF | 2.441 | 0.884 | 0.939 ± 0.46 | 9.06 ± 0.26b | 43.59 ± 2.45a |
AF | 4.874 | 1.100 | 0.419 ± 0.32 | 5.02 ± 0.18b | 15.68 ± 3.14b |
2.2. Sub-Fractions
Test materials | DPPH EC50 | ABTS IC50 | Superoxide radical (mol α-tocopherol/g DW) |
---|---|---|---|
Compound 1 | 0.087 | 0.010 | 4.348 ± 1.74 |
Compound 2 | 0.108 | 0.012 | 1.362 ± 0.58 |
Compound 3 | – | 0.138 | 0.272 ± 0.15 |
Compound 4 | 0.262 | 0.275 | 0.087 ± 0.02 |
Compound 5 | 0.535 | 0.033 | – |
Compound 6 | 0.413 | 0.040 | 0.551 ± 0.46 |
Compound 7 | – | 0.021 | 0.125 ± 0.02 |
Compound 8 | – | 0.022 | – |
Compound 9 | – | 0.046 | – |
Ascorbic acid | 0.069 | ND | ND |
Trolox® | ND | 0.089 | ND |
2.3. The Structure-Activity Relationships of Compounds Derived from D. Cambodiana
3. Experimental
3.1. General
3.2. Plant Materials
3.3. Extraction and Isolation of Antioxidant Compounds
3.4. Assays
3.4.1. DPPH scavenging capacity
3.4.2. ABTS free radical scavenging assay
3.4.3. Superoxide radical-scavenging capacity
3.4.4. Determination of the amount of total phenolics
3.4.5. Determination of the amount of total flavonoids
3.5. Statistical Analysis
4. Conclusions
Acknowledgements
- Sample Availability: Samples of the compounds 1–9 are available from the authors.
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Luo, Y.; Wang, H.; Xu, X.; Mei, W.; Dai, H. Antioxidant Phenolic Compounds of Dracaena Cambodiana. Molecules 2010, 15, 8904-8914. https://doi.org/10.3390/molecules15128904
Luo Y, Wang H, Xu X, Mei W, Dai H. Antioxidant Phenolic Compounds of Dracaena Cambodiana. Molecules. 2010; 15(12):8904-8914. https://doi.org/10.3390/molecules15128904
Chicago/Turabian StyleLuo, Ying, Hui Wang, Xuerong Xu, Wenli Mei, and Haofu Dai. 2010. "Antioxidant Phenolic Compounds of Dracaena Cambodiana" Molecules 15, no. 12: 8904-8914. https://doi.org/10.3390/molecules15128904