Preparation and Characterization of Novel Gellan Gum Hydrogels Suitable for Modified Drug Release
Abstract
:1. Introduction
2. Results and Discussion
- - physical gel of Gellan (cooling of hot solutions)
- - physical gel of Gellan in the presence of different amounts of Lys (cooling of hot solutions)
- - chemical gel of Gellan in the presence of different amounts of Lys and EDC/NHS
2.1. Structural differences between physical and chemical hydrogels
2.2. Water uptake
H2O | NaCl 0.9% w/v | SIF | SGF | |
---|---|---|---|---|
[Gellan 2%]Phys | 0.96 ± 0.03 | 0.99 ± 0.04 | 0.97 ± 0.02 | 0.77 ± 0.02 |
[Gellan 2% + Lys0.1]Phys | 1.03 ± 0.04 | 1.04 ± 0.03 | 1.02 ± 0.04 | 0.93 ± 0.02 |
[Gellan 2% + Lys 0.2]Phys | 1.02 ± 0.02 | 1.02 ± 0.04 | 1.03 ± 0.03 | 1.00 ± 0.04 |
[Gellan 2% + Lys 0.3]Phys | 0.99 ± 0.02 | 1.02 ± 0.02 | 1.02 ± 0.04 | 0.98 ± 0.02 |
[Gellan 2% + Lys 0.1]Chem | 21.95 ± 0.20 | 1.48 ± 0.03 | 1.54 ± 0.03 | 1.34 ± 0.04 |
[Gellan 2% + Lys 0.2]Chem | 12.98 ± 0.15 | 1.04 ± 0.02 | 1.02 ± 0.03 | 0.75 ± 0.03 |
[Gellan 2% + Lys 0.3]Chem | 7.06 ± 0.15 | 0.99 ± 0.04 | 0.98 ± 0.02 | 0.70 ± 0.02 |
2.3. Rheological measurements
T = 25 °C | T = 37 °C | |||
---|---|---|---|---|
σYield (Pa) | γYield | σYield (Pa) | γYield | |
[Gellan 2% + Lys 0.1]Phys | 2226 ± 509 | 0.335 ± 0.218 | 1646 ± 110 | 0.324 ± 0.003 |
[Gellan 2% + Lys 0.2]Phys | 2457 ± 1034 | 0.094 ± 0.040 | 2587 ± 698 | 0.203 ± 0.061 |
[Gellan 2% + Lys 0.3]Phys | 3296 ± 146 | 0.095 ± 0.003 | 3235 ± 76 | 0.145 ± 0.011 |
[Gellan 2% + Lys 0.1]Chem | 1130 ± 34 | 11.585 ± 0.417 | 701 ± 88 | 2.705 ± 0.358 |
[Gellan 2% + Lys 0.2]Chem | 1210 ± 314 | 2.160 ± 0.452 | 675 ± 50 | 1.782 ± 0.075 |
[Gellan 2% + Lys 0.3]Chem | 1321 ± 160 | 1.425 ± 0.219 | 770 ± 290 | 0.729 ± 0.080 |
2.4. Compressibility tests
A1 (N/m2) | A3/A1 (%) | E (N/m2) | |
---|---|---|---|
[Gellan 2% + Lys 0.1]Phys | 38950 ± 1530 | 65.6 ± 0.5 | 73830 ± 3740 |
[Gellan 2% + Lys 0.2]Phys | 72890 ± 17800 | 72.8 ± 3.7 | 154690 ± 38000 |
[Gellan 2% + Lys 0.3]Phys | 74100 ± 19400 | 87.4 ± 0.5 | 168090 ± 38290 |
[Gellan 2% + Lys 0.1]Chem | 10990 ± 1660 | 99.1 ± 0.6 | 13440 ± 1470 |
[Gellan 2% + Lys 0.2]Chem | 14100 ± 2130 | 94.2 ± 0.8 | 18470 ± 4750 |
[Gellan 2% + Lys 0.3]Chem | 17920 ± 3140 | 88.1 ± 1.2 | 24990 ± 5700 |
2.5. In vitro release studies
2.5.1. Release of vitamin B12
2.5.2. Release of fluorescein isothiocyanate-dextran
3. Experimental Section
3.1. Materials
3.2. Physical hydrogel preparation
3.3. Chemical hydrogel preparation
- cP = 20 mg/mL
- EDC] / [COOH] = 5
- EDC] / [NHS] = 1
- Lys] / [COOH] = 0.1, 0.2, 0.3
3.4. Water uptake
3.5. Rheological measurements
3.6. Compressibility measurements
3.7. In vitro release
4. Conclusions
Acknowledgements
- Samples Availability: Samples of the physical and chemical gels [Gellan 2% + Lys 0.1], [Gellan 2% + Lys 0.2] and [Gellan 2% + Lys 0.3] are available from the authors.
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Matricardi, P.; Cencetti, C.; Ria, R.; Alhaique, F.; Coviello, T. Preparation and Characterization of Novel Gellan Gum Hydrogels Suitable for Modified Drug Release. Molecules 2009, 14, 3376-3391. https://doi.org/10.3390/molecules14093376
Matricardi P, Cencetti C, Ria R, Alhaique F, Coviello T. Preparation and Characterization of Novel Gellan Gum Hydrogels Suitable for Modified Drug Release. Molecules. 2009; 14(9):3376-3391. https://doi.org/10.3390/molecules14093376
Chicago/Turabian StyleMatricardi, Pietro, Claudia Cencetti, Roberto Ria, Franco Alhaique, and Tommasina Coviello. 2009. "Preparation and Characterization of Novel Gellan Gum Hydrogels Suitable for Modified Drug Release" Molecules 14, no. 9: 3376-3391. https://doi.org/10.3390/molecules14093376