Optimization of Fermentation Conditions for Carrageenase Production by Cellulophaga Species: A Comparative Study
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Methods
2.2.1. Bacterial Strains
2.2.2. Optimization of Culture Medium by One-Factor-at-a-Time Experiment
2.2.3. Bacterial Growth Conditions
2.2.4. Determination of Carrageenase Activity
2.2.5. Effect of Temperature and pH on Enzymatic Degradation
2.2.6. Size-Exclusion Chromatography
2.2.7. Statistical Analysis
3. Results and Discussion
3.1. Phylogeny Analysis
3.2. Optimal Substrate Specificity
3.3. Optimal Growth Temperature and Incubation Time
3.4. Optimal Sea Salt Concentration
3.5. Optimal Nitrogen Source
3.6. Optimal Yeast Extract Concentration
3.7. Optimal Furcellaran Concentration
3.8. Effect of Temperature and pH on Enzymatic Degradation
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Howlader, M.M.; Molz, J.; Sachse, N.; Tuvikene, R. Optimization of Fermentation Conditions for Carrageenase Production by Cellulophaga Species: A Comparative Study. Biology 2021, 10, 971. https://doi.org/10.3390/biology10100971
Howlader MM, Molz J, Sachse N, Tuvikene R. Optimization of Fermentation Conditions for Carrageenase Production by Cellulophaga Species: A Comparative Study. Biology. 2021; 10(10):971. https://doi.org/10.3390/biology10100971
Chicago/Turabian StyleHowlader, Md Musa, Jana Molz, Nico Sachse, and Rando Tuvikene. 2021. "Optimization of Fermentation Conditions for Carrageenase Production by Cellulophaga Species: A Comparative Study" Biology 10, no. 10: 971. https://doi.org/10.3390/biology10100971