Structure and Dynamics of dsDNA in Cell-like Environments
Abstract
:1. Introduction
1.1. DNA Double-Helix Opening In Vivo
- Genetic information is coded twice in the two complementary strands. This allows storage of the “information” and the ability to check for errors during replication [9].
- The sugar–phosphate backbone promotes base-paring between complementary strands, which is essential for genetic information storage and retrieval [11].
- The lineal or stacked arrangement of the bases along the longitudinal axis of the DNA allows proteins to directly access the fragment of the sequence.
- Opening (unwinding) and closing of the two DNA strands is reversible. That is how replication and transcription can be carried out without damaging the original molecule.
1.2. DNA Double-Helix Opening In Vitro
1.2.1. Thermal Melting of DNA
1.2.2. Force-Induced Melting of DNA
2. Role of Salt Concentration
3. DNA in a Crowded Solution
4. DNA in Confined Geometry
5. Translocation of DNA (DNA Passing through Pores)
6. Conclusions and Future Directions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Singh, A.; Maity, A.; Singh, N. Structure and Dynamics of dsDNA in Cell-like Environments. Entropy 2022, 24, 1587. https://doi.org/10.3390/e24111587
Singh A, Maity A, Singh N. Structure and Dynamics of dsDNA in Cell-like Environments. Entropy. 2022; 24(11):1587. https://doi.org/10.3390/e24111587
Chicago/Turabian StyleSingh, Amar, Arghya Maity, and Navin Singh. 2022. "Structure and Dynamics of dsDNA in Cell-like Environments" Entropy 24, no. 11: 1587. https://doi.org/10.3390/e24111587
APA StyleSingh, A., Maity, A., & Singh, N. (2022). Structure and Dynamics of dsDNA in Cell-like Environments. Entropy, 24(11), 1587. https://doi.org/10.3390/e24111587