Search Results (59)

Woody plant encroachment (WPE) is transforming grasslands globally, yet accurately mapping this process remains challenging. State-funded, publicly available high-resolution aerial imagery offers a potential solution, including the USDA’s National Agriculture Imagery Program (NAIP) and NSF’s National Ecological Observatory Network (NEON) Aerial Observation Platform (AOP). We evaluated the accuracy of land cover classification using NAIP, NEON, and both sources combined. We compared two machine learning models—support vector machines and random forests—implemented in R using large training and evaluation data sets. Our study site, Konza Prairie Biological Station, is a long-term experiment in which variable fire and grazing have created mosaics of herbaceous plants, shrubs, deciduous trees, and evergreen trees (Juniperus virginiana). All models achieved high overall accuracy (>90%), with NEON slightly outperforming NAIP. NAIP underperformed in detecting evergreen trees (52–78% vs. 83–86% accuracy with NEON). NEON models relied on LiDAR-based canopy height data, whereas NAIP relied on multispectral bands. Combining data from both platforms yielded the best results, with 97.7% overall accuracy. Vegetation indices contributed little to model accuracy, including NDVI (normalized digital vegetation index) and EVI (enhanced vegetation index). Both machine learning methods achieved similar accuracy. Our results demonstrate that free, high-resolution imagery and open-source tools can enable accurate, high-resolution, landscape-scale WPE monitoring. Broader adoption of such approaches could substantially improve the monitoring and management of grassland biodiversity, ecosystem function, ecosystem services, and environmental resilience. Full article

Background: Different evidence on the ability of [¹⁸F] fluorodeoxyglucose ([¹⁸F]FDG) positron emission tomography (PET) imaging to assess patients in the vegetative state (VS) or unresponsive wakefulness syndrome (UWS) has been reported. Therefore, this systematic review aimed to synthesize the existing literature on this topic. Methods: A wide literature search of the PubMed/MEDLINE, Scopus, and Embase databases was conducted to find relevant published articles investigating the role of [¹⁸F]FDG PET imaging in the assessment of VS/UWS. Results: Thirty-seven studies were included in the review, and the main fields of application of this imaging modality in these patients were the evaluation of hypometabolic patterns, differentiation of disorders of consciousness (DOC), prognostic value, and ability to assess the response to particular stimuli. Conclusions: The possible role of [¹⁸F]FDG PET imaging in the assessment of VS/UWS has emerged, in particular in the differential diagnosis of other DOC or prognosis. Some insights into its value in stimulation response and therapy evaluation have also been proposed. Standardization of protocols and larger prospective studies are needed to strengthen these clinical recommendations. Full article

Background/Objectives: Guidelines for the diagnosis of children with disorder of consciousness (DoC) in pediatric age have not been defined yet. Assessment tools designed for adults have generally not been standardized for pediatrics, which could lead to misdiagnosis due to the limited behavioral repertoire of children. This study aims at examining the basic psychometric properties of the Italian Coma Recovery Scale for Pediatrics (CRS-P) in typically developing children. Methods: A total of 64 typically developing children aged 3 months to 5:7 years were administered the CRS-P. Performance was examined across the age range, and for the two behaviors indicating emergence to a conscious state, namely functional object use (FOU) and functional communication (FC). Results: Inter-rater reliability ranged from 0.95 to 1 for subscale and total scores. All children aged ≥34 months scored at the CRS-P ceiling. All children ≥ 14 months met the criteria for FOU and all children ≥ 34 months met those for FC. Children as early as 3 months of age displayed behaviors discriminating between vegetative state (VS) and minimally conscious state (MCS) in the Visual and Motor subscales. Language-based behaviors of MCS in other subscales were consistently displayed by older children. Conclusions: Typically developing children met the criteria for all items of the Italian CRS-P by 34 months, which suggests caution in adopting the scale at a younger age. However, the features of the distinct stages of DoC could be captured earlier, based on the various subscales. Modifications should be made to some items to improve diagnostic accuracy. Full article

Tidal marshes play a globally critical role in carbon and hydrologic cycles by sequestering carbon dioxide from the atmosphere and exporting dissolved organic carbon to connected estuaries. These ecosystems provide critical habitat to a variety of fauna and also reduce coastal flood impacts. Accurate characterization of tidal marsh inundation dynamics is crucial for understanding these processes and ecosystem services. In this study, we developed remote sensing-based inundation classifications over a range of tidal stages for marshes of the Mid-Atlantic and Gulf of Mexico regions of the United States. Inundation products were derived from C-band and L-band synthetic aperture radar (SAR) imagery using backscatter thresholding and temporal change detection approaches. Inundation products were validated with in situ water level observations and radiometric modeling. The Michigan Microwave Canopy Scattering (MIMICS) radiometric model was used to simulate radar backscatter response for tidal marshes across a range of vegetation parameterizations and simulated hydrologic states. Our findings demonstrate that inundation classifications based on L-band SAR—developed using backscatter thresholding applied to single-date imagery—were comparable in accuracy to the best performing C-band SAR inundation classifications that required change detection approaches applied to time-series imagery (90.0% vs. 88.8% accuracy, respectively). L-band SAR backscatter threshold inundation products were also compared to polarimetric decompositions from quad-polarimetric Phased Array L-band Synthetic Aperture Radar 2 (PALSAR-2) and L-band Uninhabited Aerial Vehicle Synthetic Aperture Radar (UAVSAR) imagery. Polarimetric decomposition analysis showed a relative shift from volume and single-bounce scattering to double-bounce scattering in response to increasing tidal stage and associated increases in classified inundated area. MIMICS modeling similarly showed a relative shift to double-bounce scattering and a decrease in total backscatter in response to inundation. These findings have relevance to the upcoming NASA-ISRO Synthetic Aperture Radar (NISAR) mission, as threshold-based classifications of wetland inundation dynamics will be employed to verify that NISAR datasets satisfy associated mission science requirements to map wetland inundation with classification accuracies better than 80% at 1 hectare spatial scales. Full article

Background/Objectives: Cranioplasty (CP) is the main surgical procedure aiming to repair a morphological defect in the skull. It has been shown that early CP is useful for patients with traumatic brain injury (TBI) to achieve functional recovery, whereas few studies have investigated the clinical effects of ultra-late CP on TBI outcomes. Methods: Here, we describe the clinical course over 2 years of a TBI patient who underwent CP 19 months after fronto-parietal decompressive craniectomy (DC) of a limited size. Results: We found that after ultra-late CP, a meaningful functional recovery (cognitive and motor), with emergence from a minimally conscious state and recovery of functional communication, was revealed. Conclusions: Our preliminary findings contribute to the actual debate on the timing of CP for this neurosurgical procedure’s therapeutic success, as early CP has already been shown. Full article

Reptiles are threatened by anthropogenic landscape transformation, largely due to agriculture. However, ecosystems nowadays constitute a matrix of fragmented landscapes. We analyzed a heterogeneous landscape’s reptile taxonomic and functional diversity patterns across ten land cover/use types in Jalisco state, in west-central Mexico. At the alpha diversity level, we assessed the taxonomic diversity using q-order indices, and functional diversity with multidimensional indices (FRic, FDiv, and FEve) by land cover/use. We evaluated the differences in species composition among land cover/use types (beta diversity). We utilized multidimensional distance-based analyses (dbRDA) to evaluate the association between reptile diversity, habitat structure, and environmental variables. Species richness did not correlate with functional richness across land cover types overall, except for riparian habitat surrounded by crops (RH-C), which exhibited higher species richness and functional diversity. Secondary vegetation surrounded by temperate forest (SV-TF), riparian habitat surrounded by tropical dry forest (RH-TDF), and RH-C were the land cover/use types with the most functional groups. Herbaceous cover is crucial for preserving both reptile diversity facets in this landscape. These findings suggest that the availability of resources (e.g., riparian habitat and herbaceous cover) regardless of perturbation level could be more relevant for reptile diversity than the condition (tropical vs. temperate) due to the high plasticity and adaptation of the group. It is essential to recognize the ecological value of these habitats by adopting a holistic approach that values the intrinsic and ecological importance of reptile diversity. Full article

Anaerobic digestion is an important means to turn agricultural waste into resources and an important way to address the challenges in treating vegetable residues in China. In this study, the co-digestion of dairy manure with tomato residue was investigated to clarify the effect of the total solids (TS) of the digestion substrate on methane’s production and mechanism using the self-made anaerobic digestion device. The results showed that all treatments could rapidly ferment methane and that the daily methane production showed a trend of increasing first and then decreasing. The optimal concentrations of the digestion substrate for liquid anaerobic digestion (L-AD), hemi-solid-state anaerobic digestion (HSS-AD), and solid-state anaerobic digestion (SS-AD) were 10%, 18%, and 25%, respectively. Compared with SS-AD and HSS-AD, L-AD gas production peaked 3–6 days earlier. Treatment TS25 had the best cumulative methane production, reaching 117.4 mL/g VS. However, treatment TS6 had acid accumulation and a very unstable system. The cumulative methane production of SS-AD was higher than that of HSS-AD and L-AD. Firmicutes and Bacteroidetes were the dominant flora, and Methanoculleus, Methanosarcina, and Methanobrevibacter were the main archaeal groups. The TS significantly changed the microbial community composition of the digestion system, especially the low TS treatment. The results presented herein indicated that TS significantly changed the bacterial and archaeal community composition of the digestion system, and thus with the increase in TS from 6% to 25%, the methane yield increased. Full article

Brain imaging studies have recently provided some evidence in favor of covert cognitive processes that are ongoing in patients with disorders of consciousness (DoC) (e.g., a minimally conscious state and vegetative state/unresponsive wakefulness syndrome) when engaged in passive sensory stimulation or active tasks such as motor imagery. In this exploratory study, we used transcranial magnetic stimulation (TMS) of the motor cortex to assess modulations of corticospinal excitability induced by action observation in eleven patients with DoC. Action observation is known to facilitate corticospinal excitability in healthy subjects, unveiling how the observer’s motor system maps others’ actions onto her/his motor repertoire. Additional stimuli were non-biological motion and acoustic startle stimuli, considering that sudden and loud acoustic stimulation is known to lower corticospinal excitability in healthy subjects. The results indicate that some form of motor resonance is spared in a subset of patients with DoC, with some significant difference between biological and non-biological motion stimuli. However, there was no covariation between corticospinal excitability and the type of DoC diagnosis (i.e., whether diagnosed with VS/UWS or MCS). Similarly, no covariation was detected with clinical changes between admission and discharge in clinical outcome measures. Both motor resonance and the difference between the resonance with biological/non-biological motion discrimination correlated with the amplitude of the N20 somatosensory evoked potentials, following the stimulation of the median nerve at the wrist (i.e., the temporal marker signaling the activation of the contralateral primary somatosensory cortex). Moreover, the startle-evoking stimulus produced an anomalous increase in corticospinal excitability, suggesting a functional dissociation between cortical and subcortical circuits in patients with DoC. Further work is needed to better comprehend the conditions in which corticospinal facilitation occurs and whether and how they may relate to individual clinical parameters. Full article

(1) Background: studies have shown that some patients experience mental deterioration after bariatric surgery. (2) Methods: We examined whether the use of probiotics and improved eating habits can improve the mental health of people who suffered from mood disorders after bariatric surgery. We also analyzed patients’ mental states, eating habits and microbiota. (3) Results: Depressive symptoms were observed in 45% of 200 bariatric patients. After 5 weeks, we noted an improvement in patients’ mental functioning (reduction in BDI and HRSD), but it was not related to the probiotic used. The consumption of vegetables and whole grain cereals increased (DQI-I adequacy), the consumption of simple sugars and SFA decreased (moderation DQI-I), and the consumption of monounsaturated fatty acids increased it. In the feces of patients after RYGB, there was a significantly higher abundance of two members of the Muribaculaceae family, namely Veillonella and Roseburia, while those after SG had more Christensenellaceae R-7 group, Subdoligranulum, Oscillibacter, and UCG-005. (4) Conclusions: the noted differences in the composition of the gut microbiota (RYGB vs. SG) may be one of the determinants of the proper functioning of the gut–brain microbiota axis, although there is currently a need for further research into this topic using a larger group of patients and different probiotic doses. Full article

Objective: To compare the EEG changes in vegetative state (VS) patients and non-craniotomy, non-vegetative state (NVS) patients during general anesthesia with low-dose propofol and to find whether it affects the arousal rate of VS patients. Methods: Seven vegetative state patients (VS group: five with traumatic brain injury, two with ischemic–hypoxic VS) and five non-craniotomy, non-vegetative state patients (NVS group) treated in the Department of Neurosurgery, Peking University International Hospital from January to May 2022 were selected. All patients were induced with 0.5 mg/kg propofol, and the Bispectral Index (BIS) changes within 5 min after administration were observed. Raw EEG signals and perioperative EEG signals were collected and analyzed using EEGLAB in the MATLAB software environment, time–frequency spectrums were calculated, and EEG changes were analyzed using power spectrums. Results: There was no significant difference in the general data before surgery between the two groups (p > 0.05); the BIS reduction in the VS group was significantly greater than that in the NVS group at 1 min, 2 min, 3 min, 4 min, and 5 min after 0.5 mg/kg propofol induction (p < 0.05). Time–frequency spectrum analysis showed the following: prominent α band energy around 10 Hz and decreased high-frequency energy in the NVS group, decreased high-frequency energy and main energy concentrated below 10 Hz in traumatic brain injury VS patients, higher energy in the 10–20 Hz band in ischemic–hypoxic VS patients. The power spectrum showed that the brain electrical energy of the NVS group was weakened R5 min after anesthesia induction compared with 5 min before induction, mainly concentrated in the small wave peak after 10 Hz, i.e., the α band peak; the energy of traumatic brain injury VS patients was weakened after anesthesia induction, but no α band peak appeared; and in ischemic–hypoxic VS patients, there was no significant change in low-frequency energy after anesthesia induction, high-frequency energy was significantly weakened, and a clear α band peak appeared slightly after 10 Hz. Three months after the operation, follow-up visits were made to the VS group patients who had undergone SCS surgery. One patient with traumatic brain injury VS was diagnosed with MCS-, one patient with ischemic–hypoxic VS had increased their CRS-R score by 1 point, and the remaining five patients had no change in their CRS scores. Conclusions: Low doses of propofol cause great differences in the EEG of different types of VS patients, which may be the unique response of damaged nerve cell residual function to propofol, and these weak responses may also be the basis of brain recovery Full article

Water level variability and temporal change are critical for shaping the structure of aquatic vegetation. Much research has examined the response of aquatic vegetation to hydrological metrics. However, the hydrological sequence is a fundamental driver of aquatic ecosystem structure and function. Given the aleatory uncertainty of future water levels under an unstable climate, how aquatic vegetation responds to changing dynamics in hydrological processes, especially shifting water level sequences, remains insufficiently explored. In this paper, we establish an evaluation framework to study the response of vegetation diversity to variation in water level sequences during a drought event. To do this, the uncertainty and variability of water level processes are both considered. Altering water level processes was achieved using two types of scenarios in order to explore the effects of differing water level sequences (i.e., changing the order of high vs. low water levels) on the probability distribution of four indexes of aquatic plant diversity (e.g., Margalef’s, Simpson’s, Shannon’s, and Pielou’s evenness index). Our results show that altering the order of water level state can lead to differences in the diversity of aquatic vegetation, with a pronounced impact on vegetation complexity. This suggests that the specific sequence of water level events is critical for shaping aquatic vegetation structure. In addition, we found that a uniform distribution of water level state is beneficial for enhancing a species’ dominance in aquatic vegetation. Our findings provide guidance for improving the future development of freshwater ecosystem protection and lake management. Full article

Unprecedented advancements in the diagnosis and treatment of patients with disorders of consciousness (DoC) have given rise to ethical questions about how to recognize and respect autonomy and a sense of agency of the personhood when those capacities are themselves disordered, as they typically are in patients with DoC. At the intersection of these questions rests the distinction between consciousness and unconsciousness. Indeed, evaluations of consciousness levels and capacity for recovery have a significant impact on decisions regarding whether to discontinue or prolong life-sustaining therapy for DoC patients. However, in the unconsciousness domain, there is the confusing array of terms that are regularly used interchangeably, making it quite challenging to comprehend what unconsciousness is and how it might be empirically grounded. In this opinion paper, we will provide a brief overview of the state of the field of unconsciousness and show how a rapidly evolving electroencephalogram (EEG) neuroimaging technique may offer empirical, theoretical, and practical tools to approach unconsciousness and to improve our ability to distinguish consciousness from unconsciousness and also nonconsciousness with greater precision, particularly in cases that are borderline (as is typical in patients with DoC). Furthermore, we will provide a clear description of three distant notions of (un)consciousness (unconsciousness, nonconsciousness, and subconsciousness) and discuss how they relate to the experiential selfhood which is essential for comprehending the moral significance of what makes life worth living. Full article

Obesity in the United States continues to worsen. Anthocyanin-rich fruits and vegetables provide a pragmatic dietary approach to slow its metabolic complications. Given American diet patterns, foods with high anthocyanin content could address dose-response challenges. The study objective was to determine the effect of 100% elderberry juice on measures of indirect calorimetry (IC) and insulin sensitivity/glucose tolerance in a placebo-controlled, randomized, crossover pilot study. Overweight and obese adults were randomized to a 5-week study which included 2 1-week periods of twice-daily elderberry juice (EBJ) or sugar-matched placebo consumption separated by a 3-week washout period. Following each 1-week test period, IC and insulin sensitivity/glucose tolerance was measured with a 3 h meal tolerance test (MTT). Treatment differences were tested with linear mixed modeling. A total of 22 prospective study volunteers (18 F/4 M) attended recruitment meetings, and 9 were analyzed for treatment differences. EBJ was well tolerated and compliance was 99.6%. A total of 6 IC measures (intervals) were created, which coincided with 10–20 min gaseous samplings in-between MTT blood samplings. Average CHO oxidation was significantly higher during the MTT after 1-week EBJ consumption (3.38 vs. 2.88 g per interval, EBJ vs. placebo, p = 0.0113). Conversely, average fat oxidation was significantly higher during the MTT after 1-week placebo consumption (1.17 vs. 1.47 g per interval, EBJ vs. placebo, p = 0.0189). This was in-line with a significantly lower average respiratory quotient after placebo treatment (0.87 vs. 0.84, EBJ vs. placebo, p = 0.0114). Energy expenditure was not different. There was no difference in serum glucose or insulin response between treatments. This pilot study of free-living volunteers describes significant change in IC but not insulin sensitivity with an EBJ intervention. Controlled feeding and increased sample size will help determine the utility of EBJ on these outcomes. Full article

Background: the restrictive measures that were adopted during three waves of the COVID-19 pandemic had an impact on both the emotional state and lifestyle of the general population. We evaluated the impact of COVID-19 pandemic on lifestyles and emotional states of women planning assisted reproductive technology (ART), and whether these changes affected ART outcomes. Methods: quantitative research, using a web-based survey, was performed on 289 Caucasian women. Results: In preconception, we observed higher percentage of women with positive obstetric outcomes who reduced body weight (52.4% vs. 27.2%, p = 0.09). Over 60% of women with positive outcomes practiced physical activity vs. 47% of women with negative outcomes (p = 0.03), as well as having better quality of sleep (45% vs. 35%), and a more solid relationships with their partners (65.1% vs. 51.7%, p = 0.03). Women who increased their intake of whole grains, fruits, vegetables, and legumes (p < 0.05), according to the Mediterranean diet, showed positive outcomes. We observed that participants who experienced “very much” or “extreme” anxiety, sadness, and fear (p < 0.05) during pandemic were clearly more numerous in the group with negative pregnancy outcomes. Conclusions: healthy lifestyle together with a positive emotional state in preconception can positively influence the obstetric outcomes after ART. Full article

The temperature of the brain can reflect the activity of its different regions, allowing us to evaluate the connections between them. A study involving 111 patients in a vegetative state or minimally conscious state used microwave radiometry to measure their cortical temperature. The patients were divided into a main group receiving a 10-day selective craniocerebral hypothermia (SCCH) procedure, and a control group receiving basic therapy and rehabilitation. The main group showed a significant improvement in consciousness level as measured by CRS-R assessment on day 14 compared to the control group. Temperature heterogeneity increased in patients who received SCCH, while remaining stable in the control group. The use of microwave radiometry to assess rehabilitation effectiveness and the inclusion of SCCH in rehabilitation programs appears to be a promising approach. Full article