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Keywords = termini restraining

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13 pages, 1763 KB  
Review
Protein Fusion Strategies for Membrane Protein Stabilization and Crystal Structure Determination
by Shixuan Liu and Weikai Li
Crystals 2022, 12(8), 1041; https://doi.org/10.3390/cryst12081041 - 27 Jul 2022
Cited by 12 | Viewed by 11170
Abstract
Crystal structures of membrane proteins are highly desired for their use in the mechanistic understanding of their functions and the designing of new drugs. However, obtaining the membrane protein structures is difficult. One way to overcome this challenge is with protein fusion methods, [...] Read more.
Crystal structures of membrane proteins are highly desired for their use in the mechanistic understanding of their functions and the designing of new drugs. However, obtaining the membrane protein structures is difficult. One way to overcome this challenge is with protein fusion methods, which have been successfully used to determine the structures of many membrane proteins, including receptors, enzymes and adhesion molecules. Existing fusion strategies can be categorized into the N or C terminal fusion, the insertion fusion and the termini restraining. The fusions facilitate protein expression, purification, crystallization and phase determination. Successful applications often require further optimization of protein fusion linkers and interactions, whose design can be facilitated by a shared helix strategy and by AlphaFold prediction in the future. Full article
(This article belongs to the Special Issue Membrane Protein Crystallography)
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