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Keywords = tapetal PCD

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16 pages, 5790 KiB  
Article
Cytological Characterization of vrnp 1, a Pollen-Free Male Sterile Mutant in Mung Bean (Vigna radiata)
by Yuxin Cheng, Tianjiao Lan, Kunpeng Deng, Minghai Wang, Shuying Bao, Dan Han, Yapeng Xu, Han Wang, Ning Xu and Zhongxiao Guo
Agronomy 2025, 15(2), 312; https://doi.org/10.3390/agronomy15020312 - 26 Jan 2025
Viewed by 978
Abstract
Mung bean (Vigna radiata) plays a significant role in agricultural trade, food processing and utilization, and cropping structure adjustment due to its abundant nutritional components, medicine-food homology, capacity for nitrogen fixation, and soil improvement. The low yield level is a crucial [...] Read more.
Mung bean (Vigna radiata) plays a significant role in agricultural trade, food processing and utilization, and cropping structure adjustment due to its abundant nutritional components, medicine-food homology, capacity for nitrogen fixation, and soil improvement. The low yield level is a crucial limitation factor in the mung bean industry, while heterosis is an efficient path for increasing crop yields. The flexible utilization of male sterile mung bean materials may solve this pressing demand in the industry. This study identified a completely male-sterile mutant, vrnp 1, in the EMS-mutagenized mung bean cultivar Jilv 10 population, which is controlled by a single recessive nuclear gene. Furthermore, we employed a series of microscopical and histological techniques and observed that the tapetal cells in the vrnp 1 mutant did not perform as expected when reaching stage 8 of anther development, notably exhibiting a delay in entering PCD. This was accompanied by a failure to deposit cell wall components onto the pollen wall, culminating in a complete absence of mature pollen and the manifestation of male sterility. In conclusion, the vrnp 1 mutant could potentially serve as a promising candidate for male sterility in exploiting hybrid vigor in mung bean. Our research may elucidate how the delayed initiation of programmed cell death in tapetal cells contributes to a factor implicated in mung bean male sterility. Furthermore, the phenotypic data collected during pivotal developmental phases may have contributed to a better grasp of mung bean microspores and anther development. Full article
(This article belongs to the Section Plant-Crop Biology and Biochemistry)
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17 pages, 4749 KiB  
Article
SlHB8 Is a Novel Factor in Enhancing Cold Resistance in Tomato Anthers by Modulating Tapetal Cell Death
by Hongling Guan, Canye Yu, Zaohai Zeng, Huimin Hu, Yuxiang Lin, Caiyu Wu, Yiwen Yao, Rui Xia, Zhengguo Li, Chongjian Ma, Riyuan Chen, Baowen Huang and Yanwei Hao
Int. J. Mol. Sci. 2024, 25(17), 9336; https://doi.org/10.3390/ijms25179336 - 28 Aug 2024
Viewed by 1413
Abstract
Tomato plants favor warmth, making them particularly susceptible to cold conditions, especially their reproductive development. Therefore, understanding how pollen reacts to cold stress is vital for selecting and improving cold-resistant tomato varieties. The programmed cell death (PCD) in the tapetum is particularly susceptible [...] Read more.
Tomato plants favor warmth, making them particularly susceptible to cold conditions, especially their reproductive development. Therefore, understanding how pollen reacts to cold stress is vital for selecting and improving cold-resistant tomato varieties. The programmed cell death (PCD) in the tapetum is particularly susceptible to cold temperatures which could hinder the degradation of the tapetal layer in the anthers, thus affecting pollen development. However, it is not clear yet how genes integral to tapetal degradation respond to cold stress. Here, we report that SlHB8, working upstream of the conserved genetic module DYT1-TDF1-AMS-MYB80, is crucial for regulating cold tolerance in tomato anthers. SlHB8 expression increases in the tapetum when exposed to low temperatures. CRISPR/Cas9-generated SlHB8-knockout mutants exhibit improved pollen cold tolerance due to the reduced temperature sensitivity of the tapetum. SlHB8 directly upregulates SlDYT1 and SlMYB80 by binding to their promoters. In normal anthers, cold treatment boosts SlHB8 levels, which then elevates the expression of genes like SlDYT1, SlTDF1, SlAMS, and SlMYB80; however, slhb8 mutants do not show this gene activation during cold stress, leading to a complete blockage of delayed tapetal programmed cell death (PCD). Furthermore, we found that SlHB8 can interact with both SlTDF1 and SlMYB80, suggesting the possibility that SlHB8 might regulate tapetal PCD at the protein level. This study sheds light on molecular mechanisms of anther adaptation to temperature fluctuations. Full article
(This article belongs to the Special Issue Molecular Mechanisms of Plant Abiotic Stress Tolerance: 2nd Edition)
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17 pages, 23846 KiB  
Article
Abnormal Calcium Accumulation and ROS Homeostasis-Induced Tapetal Programmed Cell Death Lead to Pollen Abortion of Petaloid-Type Cytoplasmic Male Sterility in Camellia oleifera
by Xiaolei Gao, Ying Yang, Jiawei Ye, Huan Xiong, Deyi Yuan and Feng Zou
Agronomy 2024, 14(3), 585; https://doi.org/10.3390/agronomy14030585 - 14 Mar 2024
Cited by 3 | Viewed by 1569
Abstract
Cytoplasmic male sterility (CMS) plays a crucial role in the utilization of heterosis. The petaloid anther abortion in oil tea (Camellia oleifera Abel.) constitutes a CMS phenomenon, which is of great value for the hybrid breeding of oil tea. However, as the [...] Read more.
Cytoplasmic male sterility (CMS) plays a crucial role in the utilization of heterosis. The petaloid anther abortion in oil tea (Camellia oleifera Abel.) constitutes a CMS phenomenon, which is of great value for the hybrid breeding of oil tea. However, as the mechanism of its CMS is still poorly understood, it is necessary to study the cytology and physiological characteristics of anther abortion. In this study, a C. oleifera cultivar, Huashuo (HS), and its petalized CMS mutant (HSP) were used as materials to explore this mechanism. Compared with HS, cytological analysis demonstrated that HSP showed early-onset tapetum programmed cell death (PCD) and an organelle disorder phenotype during the tetrad stage. In HSP, anthers exhibited elevated levels of calcium deposition in anther wall tissues, tapetum layers, and microspores, and yet calcium accumulation was abnormal at the later stage. The contents of hydrogen peroxide and MDA in HSP anthers were higher, and the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were lower than those of HS, which resulted in an excessive accumulation of reactive oxygen species (ROS). Real-time quantitative PCR confirmed that the transcription levels of CoPOD and CoCAT genes encoding key antioxidant enzymes in HSP were downregulated compared with HS in early pollen development; the gene CoCPK, which encodes a calcium-dependent protein kinase associated with antioxidase, was upregulated during the critical period. Thus, we suggest that excessive ROS as a signal breaks the balance of the antioxidant system, and along with an abnormal distribution of calcium ions, leads to the early initiation of PCD in the tapetum, and ultimately leads to pollen abortion for HSP. These results lay a cytological and physiological foundation for further studies on the CMS mechanism, and provide information for breeding male-sterile lines of C. oleifera. Full article
(This article belongs to the Section Crop Breeding and Genetics)
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14 pages, 1017 KiB  
Review
Cold Stress Response Mechanisms in Anther Development
by Borong Huang, Yubo Fan, Lijiao Cui, Cheng Li and Changkui Guo
Int. J. Mol. Sci. 2023, 24(1), 30; https://doi.org/10.3390/ijms24010030 - 20 Dec 2022
Cited by 22 | Viewed by 3902
Abstract
Unlike animals that can escape threats, plants must endure and adapt to biotic and abiotic stresses in their surroundings. One such condition, cold stress, impairs the normal growth and development of plants, in which most phases of reproductive development are particularly susceptible to [...] Read more.
Unlike animals that can escape threats, plants must endure and adapt to biotic and abiotic stresses in their surroundings. One such condition, cold stress, impairs the normal growth and development of plants, in which most phases of reproductive development are particularly susceptible to external low temperature. Exposed to uncomfortably low temperature at the reproductive stage, meiosis, tapetal programmed cell death (PCD), pollen viability, and fertilization are disrupted, resulting in plant sterility. Of them, cold-induced tapetal dysfunction is the main cause of pollen sterility by blocking nutrition supplements for microspore development and altering their timely PCD. Further evidence has indicated that the homeostatic imbalances of hormones, including abscisic acid (ABA) and gibberellic acid (GA), and sugars have occurred in the cold-treated anthers. Among them, cold stress gives rise to the accumulation of ABA and the decrease of active GA in anthers to affect tapetal development and represses the transport of sugar to microspores. Therefore, plants have evolved lots of mechanisms to alleviate the damage of external cold stress to reproductive development by mainly regulating phytohormone levels and sugar metabolism. Herein, we discuss the physiological and metabolic effects of low temperature on male reproductive development and the underlying mechanisms from the perspective of molecular biology. A deep understanding of cold stress response mechanisms in anther development will provide noteworthy references for cold-tolerant crop breeding and crop production under cold stress. Full article
(This article belongs to the Special Issue New Advances in Plant Abiotic Stress)
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14 pages, 5589 KiB  
Article
Abnormal Programmed Cell Death of Tapetum Leads to the Pollen Abortion of Lycium barbarum Linnaeus
by Xin Zhang, Zhanlin Bei, Haijun Ma, Zhaojun Wei, Jun Zhou, Yufeng Ren, Wendi Xu, Peng Nan, Yuguo Wang, Linfeng Li, Wenju Zhang, Ji Yang, Yang Zhong and Zhiping Song
Horticulturae 2022, 8(11), 1056; https://doi.org/10.3390/horticulturae8111056 - 9 Nov 2022
Cited by 6 | Viewed by 2080
Abstract
Programmed cell death (PCD) in tapetum provides nutrients for pollen development. Once this process becomes abnormal, the pollen will be aborted, and sterile lines will be formed. Lycium barbarum L. is a well-known medicinal and edible plant, and male sterile lines play an [...] Read more.
Programmed cell death (PCD) in tapetum provides nutrients for pollen development. Once this process becomes abnormal, the pollen will be aborted, and sterile lines will be formed. Lycium barbarum L. is a well-known medicinal and edible plant, and male sterile lines play an important role in the cultivation of its new varieties by utilizing hybrid vigor. However, the male sterility mechanism of L. barbarum is poorly understood. In this study, the main characteristic changes during the pollen development of L. barbarum sterile line (Ningqi No. 5) and fertile line (Ningqi No. 1) were compared through anatomical observation, physiological detection and gene expression analysis. Anatomical observation showed that compared with that of the fertile line, the tapetum of the sterile line persisted during pollen development, the organelle morphology and number of tapetal cells changed remarkably, and the PCD was remarkably delayed. Membranous peroxidation caused by reactive oxygen species (ROS) in the sterile line occurred from the late tetrad to the pollen grain stage, and that in Ningqi No. 1 transpired in the tetrad stage. This difference in the dynamic changes of ROS affected the redox balance of microspore development. qPCR detection of DYT1 and MS1 genes regulating tapetum development showed that compared with those in the fertile line, the expression levels of both genes in the sterile line changed significantly from pollen mother cell stage to pollen grain stage. This finding may be associated with the start-up delay of tapetal PCD. All these results suggested that abnormal tapetal PCD is an important mechanism leading to male sterility in L. barbarum. Full article
(This article belongs to the Special Issue The Response Mechanisms of Trees under Abiotic Stresses)
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20 pages, 8956 KiB  
Article
MYB2 Is Important for Tapetal PCD and Pollen Development by Directly Activating Protease Expression in Arabidopsis
by Xiaorui Guo, Lihong Li, Xiatong Liu, Chong Zhang, Xiaoyun Yao, Zhili Xun, Zhijing Zhao, Wenwen Yan, Yirong Zou, Di Liu, Hui Li and Hai Lu
Int. J. Mol. Sci. 2022, 23(7), 3563; https://doi.org/10.3390/ijms23073563 - 24 Mar 2022
Cited by 25 | Viewed by 3735
Abstract
Tapetal programmed cell death (PCD) is a complex biological process that plays an important role in pollen formation and reproduction. Here, we identified the MYB2 transcription factor expressed in the tapetum from stage 5 to stage 11 that was essential for tapetal PCD [...] Read more.
Tapetal programmed cell death (PCD) is a complex biological process that plays an important role in pollen formation and reproduction. Here, we identified the MYB2 transcription factor expressed in the tapetum from stage 5 to stage 11 that was essential for tapetal PCD and pollen development in Arabidopsis thaliana. Downregulation of MYB2 retarded tapetal degeneration, produced defective pollen, and decreased pollen vitality. EMSA and transcriptional activation analysis revealed that MYB2 acted as an upstream activator and directly regulated expression of the proteases CEP1 and βVPE. The expression of these proteases was lower in the buds of the myb2 mutant. Overexpression of either/both CEP1 or/and βVPE proteases partially recover pollen vitality in the myb2 background. Taken together, our results revealed that MYB2 regulates tapetal PCD and pollen development by directly activating expression of the proteases CEP1 and βVPE. Thus, a transcription factor/proteases regulatory and activated cascade was established for tapetal PCD during another development in Arabidopsis thaliana. Highlight: MYB2 is involved in tapetal PCD and pollen development by directly regulating expression of the protease CEP1 and βVPE and establishes a transcription factor/proteases regulatory and activated cascade. Full article
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24 pages, 7085 KiB  
Article
OsGPAT3 Plays a Critical Role in Anther Wall Programmed Cell Death and Pollen Development in Rice
by Lianping Sun, Xiaojiao Xiang, Zhengfu Yang, Ping Yu, Xiaoxia Wen, Hong Wang, Adil Abbas, Riaz Muhammad Khan, Yingxin Zhang, Shihua Cheng and Liyong Cao
Int. J. Mol. Sci. 2018, 19(12), 4017; https://doi.org/10.3390/ijms19124017 - 12 Dec 2018
Cited by 31 | Viewed by 8683
Abstract
In flowering plants, ideal male reproductive development requires the systematic coordination of various processes, in which timely differentiation and degradation of the anther wall, especially the tapetum, is essential for both pollen formation and anther dehiscence. Here, we show that OsGPAT3, a [...] Read more.
In flowering plants, ideal male reproductive development requires the systematic coordination of various processes, in which timely differentiation and degradation of the anther wall, especially the tapetum, is essential for both pollen formation and anther dehiscence. Here, we show that OsGPAT3, a conserved glycerol-3-phosphate acyltransferase gene, plays a critical role in regulating anther wall degradation and pollen exine formation. The gpat3-2 mutant had defective synthesis of Ubisch bodies, delayed programmed cell death (PCD) of the inner three anther layers, and abnormal degradation of micropores/pollen grains, resulting in failure of pollen maturation and complete male sterility. Complementation and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) experiments demonstrated that OsGPAT3 is responsible for the male sterility phenotype. Furthermore, the expression level of tapetal PCD-related and nutrient metabolism-related genes changed significantly in the gpat3-2 anthers. Based on these genetic and cytological analyses, OsGPAT3 is proposed to coordinate the differentiation and degradation of the anther wall and pollen grains in addition to regulating lipid biosynthesis. This study provides insights for understanding the function of GPATs in regulating rice male reproductive development, and also lays a theoretical basis for hybrid rice breeding. Full article
(This article belongs to the Special Issue Plant Genetics and Molecular Breeding)
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18 pages, 57494 KiB  
Article
Tapetal-Delayed Programmed Cell Death (PCD) and Oxidative Stress-Induced Male Sterility of Aegilops uniaristata Cytoplasm in Wheat
by Zihan Liu, Xiaoyi Shi, Sha Li, Gan Hu, Lingli Zhang and Xiyue Song
Int. J. Mol. Sci. 2018, 19(6), 1708; https://doi.org/10.3390/ijms19061708 - 8 Jun 2018
Cited by 51 | Viewed by 5863
Abstract
Cytoplasmic male sterility (CMS) plays a crucial role in the utilization of hybrid vigor. Pollen development is often accompanied by oxidative metabolism responses and tapetal programmed cell death (PCD), and deficiency in these processes could lead to male sterility. Aegilops uniaristata cytoplasmic male [...] Read more.
Cytoplasmic male sterility (CMS) plays a crucial role in the utilization of hybrid vigor. Pollen development is often accompanied by oxidative metabolism responses and tapetal programmed cell death (PCD), and deficiency in these processes could lead to male sterility. Aegilops uniaristata cytoplasmic male sterility (Mu-CMS) wheat is a novel male-sterile line in wheat, which possess important potential in hybrid wheat breeding. However, its CMS mechanisms remain poorly understood. In our study, U87B1-706A, with the Aegilops uniaristata cytoplasm, and the maintainer line 706B were used to explore the abortive reason. Compared with 706B, histological analysis and PCD detection of the anther demonstrated that U87B1-706A appeared as delayed tapetal PCD as well as a disorganized organelle phenotype in the early uninucleate stage. Subsequently, a shrunken microspore and disordered exine structure were exhibited in the late uninucleate stage. While the activities of antioxidase increased markedly, the nonenzymatic antioxidant contents declined obviously following overacummulation of reactive oxygen species (ROS) during pollen development in U87B1-706A. Real-time quantitative PCR testified that the transcript levels of the superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) genes, encoding pivotal antioxidant enzymes, were up-regulated in early pollen development. Therefore, we deduce excess ROS as a signal may be related to the increased expression levels of enzyme genes, thereby breaking the antioxidative system balance, resulting in delayed tapetal PCD initiation, which finally led to pollen abortion and male sterility in U87B1-706A. These results provide evidence to further explore the mechanisms of abortive pollen in CMS wheat. Full article
(This article belongs to the Section Molecular Plant Sciences)
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