Search Results (5)

Enzyme-Induced Calcium Carbonate Precipitation (EICP) shows promise for desertification control. This study investigates the effects of solid-to-liquid ratio, calcium sources, Ca²⁺ concentration, temperature, enzyme-to-liquid ratio (ELR), and pH on the activity of soybean crude urease (SCU). Furthermore, the impact of EICP treatment cycles on the mechanical properties, compressive behavior, and wind erosion resistance of aeolian sand (AS) was systematically evaluated, with microstructural evolution and pore characteristics of cemented specimens analyzed through SEM and X-CT. Key findings reveal that SCU activity and the calcium carbonate precipitation rate (PR) reached optimal levels (80~99%) under conditions of a 1:10 solid-to-liquid ratio, 1.0~1.5 M CaCl₂ concentration, 35~70 °C temperature range, and pH 7. After seven EICP treatments, AS specimens exhibited complete cementation with an unconfined compressive strength (UCS) of 580 kPa and a reduced wind erosion rate of 0.151 g/min, effectively mitigating desertification. SEM and X-CT analyses confirmed significant pore infilling and bridging between particles, accompanied by a reduction in pore quantity and permeability coefficient by over two orders of magnitude. EICP demonstrates notable advantages in enhancing mechanical performance, environmental compatibility, and cost efficiency, positioning cemented AS as a viable construction material while offering insights for sand stabilization engineering. These findings provide essential technical support for material innovation, wind and sand disaster prevention, and the sustainable construction of desert highway bases and subbases. Full article

Enzyme-induced carbonate precipitation has been studied for wind erosion control in arid areas. A comparative study was conducted between the pure urease- and crude soybean urease-induced carbonate precipitation methods with the same enzyme activity for enhancing the wind erosion resistance of desert sand. Tube tests were carried out to monitor the amount of organic matter and CaCO₃ precipitates at different reaction times. Two groups of sand specimens received several cycles of treatment with soybean urease (SU) and pure urease (PU), respectively, with urea or without urea. The treated specimens were exposed to wind-blown sand flow to evaluate erosion resistance. The results showed that SU induced more organic precipitation under the salting-out effect, which was 9.88 times higher than that from PU. Under the one-cycle treatment, SU-treated specimens with higher contents of CaCO₃ and organic matter exhibited lower erosion mass. Under the multiple-cycle treatment, the high viscosity of SU and rapid precipitation of organic matter resulted in the inhomogeneous distribution of CaCO₃ (more precipitation at the top). Once the top of SU-treated specimens was eroded, the sand below the top layer was lost rapidly, causing the erosion mass of PU-treated specimens to be 95% lower than that of SU-treated specimens. Full article

Enzyme-induced carbonate precipitation (EICP) is a new biogeotechnical ground improvement technique that uses calcium carbonate (CaCO₃) formed by biochemical processes to increase soil strength and stiffness. In this paper, crude urease extracted from soybeans was employed to catalyze the precipitation of CaCO₃ in sand. To optimize the urease extraction efficiency, factors affecting the soybean crude urease extraction, including the powdered soybean particle size, concentration, soaking time, and soaking temperature, were addressed. This paper also provided further insight regarding the impact of the urease activity of soybean crude extract on the chemical conversion efficiency and the biocementation performance in EICP. The findings revealed that the powdered soybean concentration and the particle size were the two most important factors affecting the urease activity of the soybean crude extract. The enzyme activity utilized in the EICP process might further lead to different reactant efficiencies of urea-CaCl₂ solution, and consequently, the improvement in the physical and mechanical properties of biocemented sand. Considering the chemical conversion efficiency and the biocementation performance, 60 g/L of powdered soybean was concluded as the preferred quantity for extracting the crude urease, with an enzyme activity of 6.62 mM urea min⁻¹. Under this condition, a chemical conversion efficiency of approximately 95% for 0.5 M urea-0.5 M CaCl₂ could be obtained in merely 12 h, and the unconfined compressive strength (UCS) of the EICP-treated sand exceeded 4 MPa with a CaCO₃ content of ~8%. As a high-efficient cost-effective alternative to the purified enzyme for carbonate precipitation, the soybean crude urease showed great potential for ground improvement. Full article

Soybean meal (SBM) is the most important plant protein source in animal feed. This study investigated the characteristics of different SBMs, produced by soybeans from America and Brazil (SBM-A and SBM-B) in 2017–2021 under the same controlled conditions. The effects of different SBMs on the growth performance of Nile tilapia (Oreochromis niloticus, GIFT) and apparent digestibility coefficients (ADCs) of nutrients and energy were studied. The results showed that protein dispersibility index (PDI), urease activity (UA), glycinin and fiber were the four primary key indicators for distinguishing the characteristics of the tested SBMs. The meta-analysis results suggested that UA, glycinin, and fiber showed a negative effect on the survival rate (SR) and weight gain rate (WGR) of the Nile tilapia, whereas β-conglycinin, PDI, and nitrogen solubility index (NSI) had a positive effect on the SR and WGR of the fish. The ADCs of dry matter, the gross energy, phosphorus, crude protein, valine (Val), lysine (Lys), histidine (His), serine (Ser), and glutamate (Glu) of the Diet-A group (SBM-A inclusion) were significantly higher than those in the Diet-B group (SBM-B inclusion) (p < 0.05). However, no significant difference was found in ADCs of macro-nutrients between the two SBMs (p > 0.05). Overall, PDI, UA, glycinin, and fiber were the main indicators reflecting the characteristics of the tested SBMs, and UA, glycinin, β-conglycinin, and PDI had the greatest impact on the growth performance of Nile tilapia in this study. PDI was a more sensitive indicator than NSI for representing the protein quality of SBM. Full article

Organic soil has a high content of water and compressibility. Besides that, it has a low specific gravity, density, and shear strength. This study evaluates the applicability of the soybean crude urease for calcite precipitation (SCU-CP) method and its effectiveness in organic soil as a soil-amelioration technique. Various soybean concentrations were mixed with a reagent composed of urea and calcium chloride to produce the treatment solution. Its effect on the hydrolysis rate, pH, and amount of precipitated calcite was evaluated through test-tube experiments. SEM-EDS tests were performed to observe the mineralogy and morphology of the untreated and treated samples. The treatment solution composed of the reagent and various concentrations of soybeans was applied to organic soil. The increasing strength of the organic soil was evaluated using direct shear (DS) and unconfined compression (UCS) tests. The test-tube results show that a hydrolysis rate of 1600 u/g was obtained when using 50 g/L of soybeans with a precipitation ratio of 100%. The mechanical tests show a significant enhancement in the parameters of the organic soil’s shear strength. A shear strength improvement of 50% was achieved in this study. A UCS of 148 kPa and cohesion of 50 kPa was obtained in the treated samples of organic soil. This research elucidates that the SCU-CP is an effective technique for improving organic soil’s shear strength. Full article