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In suspended mussel farms, culture-layer food supply can be limited, whereas the 4–8 m subsurface layer is rich in particulate organic matter and seston. Using a representative aquaculture block off Gouqi Island, China, this study examined the hydrodynamic and transport effects of non-uniform hanging-depth layouts. The 0° uniform-depth layout was the reference. Five configurations were modeled: V-shaped, inverted V-shaped, uniform-depth (90° inflow), V-shaped (extended), and inverted V-shaped (extended). Flume PIV validated the model. Simulations used uniform and measured velocity-profile inflows, with and without density stratification; a passive tracer tracked seston-rich water from the 4–8 m layer. Uniform-depth hanging formed a low-velocity zone in the sleeve layer, limiting water exchange. Non-uniform layouts altered drag distribution and promoted tracer redistribution into overlying 3–5 m waters. In the V-shaped configuration, water was guided upward along sleeve bottoms in the downstream half of the aquaculture block (S2), where pronounced redistribution within the aquaculture block resulted in a tracer concentration of approximately 0.1362 in the 2–3 m layer. Stratification constrained upward spreading, whereas extended configurations may limit food replenishment through enhanced upper-layer blockage and filtering effects. Low-Richardson-number regions corresponded with tracer uplift and enhanced spreading, indicating local shear. Overall, the V-shaped configuration balanced in-farm replenishment, downstream transport, and flow maintenance without additional sleeve length. Full article