Search Results (5)

This study investigated the influence of soaking and spraying with a sodium selenite (Na₂SeO₃) solution on selenium accumulation, γ-aminobutyric acid (GABA) content, phenolic compositions, and the antioxidant activity of foxtail millet sprouts. The screening results showed that foxtail millet seeds soaked with 60 mg/L of Na₂SeO₃ solution and sprayed with 2 mg/L of Na₂SeO₃ solution were the appropriate concentrations for the germination process. Compared with the spraying method, a presoaking treatment presented far higher selenium content and significantly higher (p < 0.05) selenium enrichment rates in foxtail millet sprouts. The content of free and bound phenolics, as well as GABA, were significantly (p < 0.05) increased in foxtail millet sprouts through both soaking and spraying treatments. Correspondingly, most of the individual phenolic compounds were significantly (p < 0.05) increased, especially after germination for 3 days. Trans-ferulic acid and trans-p-coumaric acid were the predominate bound phenolic acids, feruloylquinic acid and 4-p-coumaroylquinic acid were the major free-form phenolic compounds, and N-feruloyl serotonin and N-(p-coumaroyl) serotonin were the new arising phenolic derivatives caused by germination. Both the soaking and spraying treatments induced the enrichment of these individual phenolic compositions, thus increasing the total phenolic content and in vitro antioxidant activity of foxtail millet sprouts. It was indicated that selenium-enriched germination treatment should be an effective method to produce functional selenium-enriched foxtail millet sprouts with more abundant GABA and polyphenols, thus enhancing the health benefits and added value of foxtail millet. Full article

Selenium (Se) biofortification by seed treatments has been poorly explored in wheat due to the difficulties in establishing seed treatments without compromising plant productive traits. We investigated the effects of Se seed soaking as a pre-sowing treatment in bread wheat. Five soaking periods and six Se concentrations were assessed on germination and seedling traits and compared to unsoaked seeds. Twelve hours of soaking was found beneficial for most tested Se concentrations. Then, we evaluated the effects of untreated, 0, 2.5 and 25 mM Se in 12 h seed soaking treatments along the wheat crop cycle under water-deficit (WD) and well-watered (WW) conditions in a pot experiment. Our results evidenced that 12 h of 2.5 mM Se soaking did not affect the germination percentage, and speed-up seedling emergence resulted in a considerable Se seed uptake. These plants also displayed enhanced antioxidant capacity and vegetative biomass accumulation, especially under WD. The treatment with 25 mM of Se negatively affected aerial biomass, suggesting potential toxicity. Physiological responses of Se-treated plants remained unchanged, as well as grain traits. Altogether, we propose that 12 h soaking with 2.5 mM Se is a promissory pre-sowing approach to enrich bread wheat grain and straw, particularly under water-limited environments. Full article

Selenium (Se) biofortification during seed germination is important not only to meet nutritional demands but also to prevent Se-deficiency-related diseases by producing Se-enriched foods. In this study, we evaluated effects of Se biofortification of soybeans on the Se concentration, speciation, and species transformation as well as nutrients and bioactive compounds in sprouts during germination. Soybean (Glycine max L.) seedlings were cultivated in the dark in an incubator with controlled temperature and water conditions and harvested at different time points after soaking in Se solutions (0, 5, 10, 20, 40, and 60 mg/L). Five Se species and main nutrients in the sprouts were determined. The total Se content increased by 87.3 times, and a large portion of inorganic Se was transformed into organic Se during 24 h of germination, with 89.3% of the total Se was bound to soybean protein. Methylselenocysteine (MeSeCys) and selenomethionine (SeMet) were the dominant Se species, MeSeCys decreased during the germination, but SeMet had opposite trend. Se biofortification increased contents of total polyphenol and isoflavonoid compounds and amino acids (both total and essential), especially in low-concentration Se treatment. In conclusion, Se-enriched soybean sprouts have promising potential for Se supplementation and as functional foods. Full article

Rhizoctonia root-rot disease causes severe economic losses in a wide range of crops, including Vicia faba worldwide. Currently, biosynthesized nanoparticles have become super-growth promoters as well as antifungal agents. In this study, biosynthesized selenium nanoparticles (Se-NPs) have been examined as growth promoters as well as antifungal agents against Rhizoctonia solani RCMB 031001 in vitro and in vivo. Se-NPs were synthesized biologically by Bacillus megaterium ATCC 55000 and characterized by using UV-Vis spectroscopy, XRD, dynamic light scattering (DLS), and transmission electron microscopy (TEM) imaging. TEM and DLS images showed that Se-NPs are mono-dispersed spheres with a mean diameter of 41.2 nm. Se-NPs improved healthy Vicia faba cv. Giza 716 seed germination, morphological, metabolic indicators, and yield. Furthermore, Se-NPs exhibited influential antifungal activity against R. solani in vitro as well as in vivo. Results revealed that minimum inhibition and minimum fungicidal concentrations of Se-NPs were 0.0625 and 1 mM, respectively. Moreover, Se-NPs were able to decrease the pre-and post-emergence of R. solani damping-off and minimize the severity of root rot disease. The most effective treatment method is found when soaking and spraying were used with each other followed by spraying and then soaking individually. Likewise, Se-NPs improve morphological and metabolic indicators and yield significantly compared with infected control. In conclusion, biosynthesized Se-NPs by B. megaterium ATCC 55000 are a promising and effective agent against R. solani damping-off and root rot diseases in Vicia faba as well as plant growth inducer. Full article

The elements selenium (Se) and iodine (I) are both crucial for the normal functioning of the thyroid. Biofortification with these elements is particularly feasible in areas where they show a deficit. Iodine and selenium can have positive effects on different plants when applied at the correct concentrations. The effects of their simultaneous addition on plant physiology and biochemistry, as well as on seed germination and sprout biomass, were studied in pumpkin (Cucurbita pepo L. ssp. pepo). To study the effect of Se and I on sprouts, sprouts were grown from seeds soaked in solutions of different forms of Se, I and their combination in the growth chamber experiment. In the field experiment, pumpkins plants were foliarly treated with the same concentrations and forms of Se and I. The combination of Se and I treatments enhanced the germination of the soaked seeds, with no significant differences between Se and I treatments for sprout mass. The yield of pumpkins and seed production were unaffected by Se and I foliar application. The anthocyanin levels and respiratory potential measured via the electron transport system’s activity showed different patterns according to treatments and plant parts (sprouts, leaves, seeds). The redistribution of Se and I from seeds to sprouts was significant. The accumulation of Se was higher in sprouts from the seeds treated with Se together with I, compared to sprouts from the seeds treated with Se alone. Interactions between Se and I were also noted in the seeds, which developed in the treated plants. Full article