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Search Results (7)

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Keywords = quantitative light-induced fluorescence-digital

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10 pages, 1032 KiB  
Article
Validation of Quantitative Light-Induced Fluorescence Digital Analysis for Assessing Early Dental Caries Depth: A Micro-Computed Tomography-Based In Vitro Study
by Ji-Hyun Min
Dent. J. 2025, 13(3), 90; https://doi.org/10.3390/dj13030090 - 20 Feb 2025
Cited by 1 | Viewed by 1137
Abstract
Background/Objectives: This study aimed to evaluate the analytical validity of micro-computed tomography (µCT) and compare it with quantitative light-induced fluorescence digital (QLFD) for assessing early dental caries (EDC) lesion depth (LD). This study was conducted in a destructive in vitro setting. Methods: EDC [...] Read more.
Background/Objectives: This study aimed to evaluate the analytical validity of micro-computed tomography (µCT) and compare it with quantitative light-induced fluorescence digital (QLFD) for assessing early dental caries (EDC) lesion depth (LD). This study was conducted in a destructive in vitro setting. Methods: EDC lesions were artificially induced in 28 bovine tooth specimens for 10, 20, 30, 40, 50, 60, and 70 days, respectively. The lesions were analyzed using µCT and QLFD, with grayscale intensity thresholds set at 90% and 95%. A Pearson correlation analysis was performed to evaluate the relationship between lesion depths measured using micro-computed tomography (LDµCT) and quantitative light-induced fluorescence digital (LDQLFD) across different demineralization periods. Additionally, a Bland–Altman analysis was conducted to assess agreement between the two methods and identify systematic differences and potential bias. A regression analysis was further conducted with LDQLFD as the independent variable and LDµCT as the dependent variable. Results: LDµCT was consistently lower than LDQLFD across all demineralization periods. The Pearson correlation analysis demonstrated a strong correlation between LDµCT and LDQLFD (r = 0.898–0.977, p < 0.001). The Bland–Altman analysis demonstrated a high level of agreement, with most data points falling within the 95% limit of agreement. Furthermore, the regression analysis suggested that QLFD may serve as a reliable complementary tool for lesion depth assessment. Conclusions: Although µCT and QLFD operate based on different principles, the findings of this study, along with the cost-effectiveness and ease of use of QLFD, suggest that QLFD may serve as a complementary tool to µCT in experimental research. Full article
(This article belongs to the Special Issue Women's Research in Dentistry)
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12 pages, 3864 KiB  
Article
Antibacterial Effects of Paeonia lactiflora Extract on Oral Microcosm Biofilms
by Ji-Won Lim, Min-Kyung Kang and Hee-Eun Kim
Appl. Sci. 2024, 14(23), 11290; https://doi.org/10.3390/app142311290 - 3 Dec 2024
Cited by 1 | Viewed by 865
Abstract
The aim of this study was to evaluate the antibacterial effects of Paeonia lactiflora (PL) extract on oral microcosm biofilms and determine its usefulness as a natural alternative to current antimicrobial agents. Oral biofilms were cultivated on hydroxyapatite disks using human saliva for [...] Read more.
The aim of this study was to evaluate the antibacterial effects of Paeonia lactiflora (PL) extract on oral microcosm biofilms and determine its usefulness as a natural alternative to current antimicrobial agents. Oral biofilms were cultivated on hydroxyapatite disks using human saliva for 2 days, following which they were treated with 1.5 mL of distilled water (DW), 30 μg/mL of PL extract, or 0.12% chlorhexidine gluconate (CHX) once daily for 3 days. Antibacterial effects were assessed by measuring red fluorescence intensity (RatioR/G), bacterial viability (RatioG/G+R), and aciduric bacterial counts. RatioR/G was evaluated using quantitative light-induced fluorescence digital imaging. RatioR/G and RatioG/G+R in the PL group were 0.94-fold and 0.73-fold lower than those in the DW group (p < 0.001 and p = 0.011, respectively) and comparable to those in the CHX group (p = 0.356 and p = 0.964, respectively). Aciduric bacterial counts in the PL group were 0.97-fold lower than those in the DW group (p < 0.001), with no significant difference from those in the CHX group (p = 0.892). Thus, the antibacterial effect of PL extract was comparable to that of CHX; this suggests its potential as a natural alternative for managing oral biofilm-associated diseases. Full article
(This article belongs to the Special Issue Technological Innovations and Tools in Dental Practice)
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12 pages, 3844 KiB  
Article
Efficacy of Cold Atmospheric Plasma on Pathogenicity of Oral Microcosm Biofilms
by Hee-Eun Kim
Appl. Sci. 2024, 14(3), 1211; https://doi.org/10.3390/app14031211 - 31 Jan 2024
Cited by 3 | Viewed by 1353
Abstract
This study aimed to compare the longitudinal efficacy between chlorhexidine gluconate (CHX; 0.12%) and cold atmospheric plasma (CAP) in reducing oral biofilm pathogenicity, utilizing a quantitative light-induced fluorescence-digital (QLF-D) camera. Oral microcosm biofilms were developed for 2 days on 57 hydroxyapatite disks. These [...] Read more.
This study aimed to compare the longitudinal efficacy between chlorhexidine gluconate (CHX; 0.12%) and cold atmospheric plasma (CAP) in reducing oral biofilm pathogenicity, utilizing a quantitative light-induced fluorescence-digital (QLF-D) camera. Oral microcosm biofilms were developed for 2 days on 57 hydroxyapatite disks. These biofilms were treated with distilled water for 1 min, CHX for 1 min, and CAP for 2 min over the course of 6 days. The red fluorescence intensities of the biofilms were measured using a QLF-D and expressed as pre- and post-treatment red/green ratios (RatioR/G). The bacterial viability (ratio of the green-stained area to the total stained area, RatioG/G+R) was calculated using live/dead bacterial staining; the total and aciduric bacterial counts were determined. A significant intergroup difference was found between RatioR/G changes according to the treatment period (p < 0.001). The RatioR/G observed within the CAP-treated group was significantly lower compared with the CHX-treated group at every interval of measurement (p < 0.001). The CAP-treated group also exhibited a lower RatioG/G+R and more weakened bacterial aggregation compared with the CHX-treated group (p < 0.05). In the group treated with CAP, the counts of both total and aciduric bacteria were substantially reduced compared with the DW group, with a statistically significant reduction (p < 0.001). Therefore, CAP may be more effective in minimizing oral microcosm biofilm pathogenicity than 0.12% CHX. Full article
(This article belongs to the Special Issue Oral Microbial Communities and Oral Health (3rd Edition))
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11 pages, 5006 KiB  
Article
Detection of Erosive Changes on Smooth Surfaces with and without Orthodontic Brackets Using an Intraoral Scanner—An In Vitro Study
by Anahita Jablonski-Momeni, Franka Hanselmann, Peter Bottenberg and Heike Korbmacher-Steiner
Diagnostics 2023, 13(20), 3232; https://doi.org/10.3390/diagnostics13203232 - 17 Oct 2023
Cited by 1 | Viewed by 1362
Abstract
Background: Consumption of acidic beverages favours development of erosions. Modern diagnostic methods are required to detect erosions at an early stage. This study aimed to evaluate the suitability of an intraoral scanner (IOS) for detection of erosive changes on smooth surfaces adjacent to [...] Read more.
Background: Consumption of acidic beverages favours development of erosions. Modern diagnostic methods are required to detect erosions at an early stage. This study aimed to evaluate the suitability of an intraoral scanner (IOS) for detection of erosive changes on smooth surfaces adjacent to orthodontic brackets. Methods: Orthodontic metal brackets were attached to the buccal surfaces of 58 extracted permanent human teeth. Teeth were randomly divided into groups: A = 6% citric acid, B = Coca-Cola, C = Redbull, D = Powerade, E = Control, no acid exposure. Teeth were exposed to acid in three erosion cycles, followed by rinsing and brushing. Scans of teeth were performed at baseline and after each erosion cycle and enamel loss was measured. Quantitative light-induced fluorescence (QLF) measurements were performed as reference standard. Results: Significant substance loss was measured in all acid groups after the second and third erosion cycle (p < 0.0001). Correlation between scans and QLF were significant (p = 0.001). Conclusions: With IOS, it was possible to detect and quantify enamel erosion at smooth surfaces with and without orthodontic brackets after a short exposure time. Considering the limitations of in vitro results, the use of IOS can be a promising digital tool to detect and monitor erosive enamel changes during fixed orthodontic treatment. Full article
(This article belongs to the Section Medical Imaging and Theranostics)
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12 pages, 5467 KiB  
Article
Effectiveness of an Oral Health Education Program Using a Smart Toothbrush with Quantitative Light-Induced Fluorescence Technology in Children
by Jihyun Lee, Taeyang Lee, Hoi-In Jung, Wonse Park and Je Seon Song
Children 2023, 10(3), 429; https://doi.org/10.3390/children10030429 - 22 Feb 2023
Cited by 10 | Viewed by 6839
Abstract
This randomized controlled trial aimed to educate patients and manage oral hygiene remotely via a smart toothbrush (ST) by using mobile applications and to improve oral hygiene and habits by evaluating the plaque state via images from a Q-ray cam, which is a [...] Read more.
This randomized controlled trial aimed to educate patients and manage oral hygiene remotely via a smart toothbrush (ST) by using mobile applications and to improve oral hygiene and habits by evaluating the plaque state via images from a Q-ray cam, which is a quantitative light-induced fluorescence (QLF) digital imaging device. In total, 68 children (aged 6–12 years) were randomly divided into three groups. Group I was assigned an electro-toothbrush (ET), group II was assigned a smart toothbrush (ST), and group III used a manual toothbrush (MT). Each group used an assigned toothbrush and was checked after 1 and 3 months. Oral hygiene status was evaluated using the patient hygiene performance (PHP) index and fluorescent plaque index (FPI), which are presented in the QLF program. In addition, questionnaires on oral health behaviors and attitudes were also evaluated. There was a 0.24 decrease in the PHP index and a 1.40 decrease in the simple hygiene score three months later compared with the baseline in the ST group, with no significant difference between the three methods (p = 0.518, p = 0.626). Many group II participants said that ST helped with oral hygiene, and they were willing to use it continuously. In addition, all participants’ brushing times and attitudes toward oral hygiene improved after oral hygiene education using a Q-ray cam. Therefore, the use of STs provided good dental health education and a plaque-decreasing effect to children aged 6–12 years old. Furthermore, the QLF device can be used as a useful tool for maintaining good oral hygiene in children. Full article
(This article belongs to the Section Pediatric Dentistry & Oral Medicine)
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10 pages, 2696 KiB  
Article
Antimicrobial Effects of Non-Thermal Atmospheric Pressure Plasma on Oral Microcosm Biofilms
by Jiyeon Lee, Sungbo Cho and Hee-Eun Kim
Int. J. Environ. Res. Public Health 2023, 20(3), 2447; https://doi.org/10.3390/ijerph20032447 - 30 Jan 2023
Cited by 5 | Viewed by 1982
Abstract
We comparatively evaluated the antibacterial effects of non-thermal atmospheric pressure plasma (NTAPP) on oral microcosm biofilms. Oral microcosm biofilms, which are derived from inoculation with human saliva, were cultured on 48 hydroxyapatite disks for 6 days. The prepared biofilms were divided into three [...] Read more.
We comparatively evaluated the antibacterial effects of non-thermal atmospheric pressure plasma (NTAPP) on oral microcosm biofilms. Oral microcosm biofilms, which are derived from inoculation with human saliva, were cultured on 48 hydroxyapatite disks for 6 days. The prepared biofilms were divided into three different daily treatment groups: distilled water for 1 min, 0.12% chlorhexidine (CHX) for 1 min, and NTAPP for 5 min. Using a quantitative light-induced fluorescence-digital camera, the red fluorescence intensity of the biofilms was measured as red/green ratios (RatioR/G) before and after treatment. Total and aciduric bacteria were counted as colony-forming units. Using live/dead bacterial staining, bacterial viability was calculated as the RatioG/G+R. RatioR/G was approximately 0.91-fold lower in the NTAPP group than in the CHX group on day 1 of treatment (p = 0.001), and approximately 0.94-fold lower on both days 2 and 3 (p < 0.001). The number of total bacteria was higher in the NTAPP group than in the CHX group, but not significantly different. The number of aciduric bacteria was lowest in the CHX group (p < 0.001). However, bacterial viability was lowest in the NTAPP group. Restricted bacterial aggregation was observed in the NTAPP group. These findings suggest that NTAPP may more effectively reduce the pathogenicity of oral microcosm biofilms than 0.12% CHX. Full article
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10 pages, 2406 KiB  
Article
Photoinhibition of Streptococcus mutans Biofilm-Induced Lesions in Human Dentin by Violet-Blue Light
by Grace Gomez Felix Gomez, Frank Lippert, Masatoshi Ando, Andrea F. Zandona, George J. Eckert and Richard L. Gregory
Dent. J. 2019, 7(4), 113; https://doi.org/10.3390/dj7040113 - 11 Dec 2019
Cited by 10 | Viewed by 4031
Abstract
This in vitro study determined the effectiveness of violet-blue light on Streptococcus mutans (UA159) biofilm induced dentinal lesions. Biofilm was formed on human dentin specimens in a 96-well microtiter plate and incubated for 13 h in the presence of tryptic soy broth (TSB) [...] Read more.
This in vitro study determined the effectiveness of violet-blue light on Streptococcus mutans (UA159) biofilm induced dentinal lesions. Biofilm was formed on human dentin specimens in a 96-well microtiter plate and incubated for 13 h in the presence of tryptic soy broth (TSB) or TSB supplemented with 1% sucrose (TSBS). Violet-blue light (405 nm) from quantitative light-induced fluorescence (QLFTM) was used to irradiate the biofilm. Supernatant liquid was removed, and the biofilm was irradiated continuously with QLF for 5 min twice daily with an interval of 6 h for 5 d, except with one treatment on the final day. Colony forming units (CFU) of the treated biofilm, changes in fluorescence (∆F; QLF-Digital BiluminatorTM), lesion depth (L), and integrated mineral loss (∆Z; both transverse microradiography) were quantified at the end of the fifth day. Statistical analysis used analysis of variance (ANOVA), testing at a 5% significance level. In the violet-blue light irradiated groups, there was a significant reduction (p < 0.05) of bacterial viability (CFU) of S. mutans with TSB and TSBS. Violet-blue light irradiation resulted in the reduction of ∆F and L of the dentinal surface with TSBS. These results indicate that violet-blue light has the capacity to reduce S. mutans cell numbers. Full article
(This article belongs to the Special Issue Lasers in Dentistry: Hard and Soft Tissues)
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