Search Results (5)

Pithiness is one of the physiological diseases of radishes, which is accompanied by the accumulation of reactive oxygen species (ROS) during the sponging of parenchyma tissue in the fleshy roots. A respiratory burst oxidase homolog (Rboh, also known as NADPH oxidase) is a key enzyme that catalyzes the production of ROS in plants. To understand the role of Rboh genes in radish pithiness, herein, 10 RsRboh gene families were identified in the genome of Raphanus sativus using Blastp and Hmmer searching methods and were subjected to basic functional analyses such as phylogenetic tree construction, chromosomal localization, conserved structural domain analysis, and promoter element prediction. The expression profiles of RsRbohs in five stages (Pithiness grade = 0, 1, 2, 3, 4, respectively) of radish pithiness were analyzed. The results showed that 10 RsRbohs expressed different levels during the development of radish pithiness. Except for RsRbohB and RsRbohE, the expression of other members increased and reached the peak at the P2 (Pithiness grade = 2) stage, among which RsRbohD1 showed the highest transcripts. Then, the expression of 40 genes related to RsRbohD1 and pithiness were analyzed. These results can provide a theoretical basis for improving pithiness tolerance in radishes. Full article

We aimed to explore the potential link of maternal age at menarche (mAAM) gene polymorphisms with risk of the fetal growth restriction (FGR). This case (FGR)–control (FGR free) study included 904 women (273 FGR and 631 control) in the third trimester of gestation examined/treated in the Departments of Obstetrics. For single nucleotide polymorphism (SNP) multiplex genotyping, 50 candidate loci of mAAM were chosen. The relationship of mAAM SNPs and FGR was appreciated by regression procedures (logistic/model-based multifactor dimensionality reduction [MB-MDR]) with subsequent in silico assessment of the assumed functionality pithy of FGR-related loci. Three mAAM-appertain loci were FGR-linked to genes such as KISS1 (rs7538038) (effect allele G-odds ratio (OR)_allelic = 0.63/p_perm = 0.0003; OR_additive = 0.61/p_perm = 0.001; OR_dominant = 0.56/p_perm = 0.001), NKX2-1 (rs999460) (effect allele A-OR_allelic = 1.37/p_perm = 0.003; OR_additive = 1.45/p_perm = 0.002; OR_recessive = 2.41/p_perm = 0.0002), GPRC5B (rs12444979) (effect allele T-OR_allelic = 1.67/p_perm = 0.0003; OR_dominant = 1.59/p_perm = 0.011; OR_additive = 1.56/p_perm = 0.009). The haplotype ACA FSHB gene (rs555621*rs11031010*rs1782507) was FRG-correlated (OR = 0.71/p_perm = 0.05). Ten FGR-implicated interworking models were founded for 13 SNPs (p_perm ≤ 0.001). The rs999460 NKX2-1 and rs12444979 GPRC5B interplays significantly influenced the FGR risk (these SNPs were present in 50% of models). FGR-related mAAM-appertain 15 polymorphic variants and 350 linked SNPs were functionally momentous in relation to 39 genes participating in the regulation of hormone levels, the ovulation cycle process, male gonad development and vitamin D metabolism. Thus, this study showed, for the first time, that the mAAM-appertain genes determine FGR risk. Full article

In the discipline of architecture, there is an established familiarity with the 19th century’s Louis Sullivan’s pithy dictum “form follows function”. The expression has indeed directly and indirectly inspired many authors and movements, especially during the beginning of the 20th century, when objectivity showed its value in improving the progress of the industrial society. Nonetheless, the reception effects of such architecture with the primacy of function were responsible for decisive transformations in architectural form, human behaviour, social transformations and material and technological manoeuvres and gave rise to very rich developments related to form, history and inhabitants’ psychological engagements, among many others. So, what about the reception effects framed in the natural and inhabited environment? Could we make space for a sense of greater need, facing a climate emergency? The present paper brings the example of a bunker’s super-resistant heritage, as a paradigmatic sample of material resistance, that supports the idea that “Function (can) Follow the Form” when re-signifying hard architecture, as is the case with Plan Barron of Defence of Lisbon and Setubal, a recently declassified military heritage set of buildings. The study conducts a critical literature review as a qualitative method of research that groups factors into clusters to give evidence to some conceptual theoretical frameworks: “hardness”; “inheritance”; “object trouvé”; “affordance”; and “empathy”. These concepts become then the basis to frame a new paradigm: function follows form can be a pertinent approach when dealing with super-resistant structures in the present climate crisis. This inverse paragon, well explained, could work as a motto to architects for a new era of global climate action. Full article

Radish, one of the important root vegetables, is widely grown in the world due to its easy cultivation, short duration, growing habit, and adaptability to various growing conditions. However, it is still extremely difficult to produce good quality radish roots due to its vulnerability to different preharvest physiological disorders. Important physiological disorders that significantly reduce the yield and quality of radish are forking, pithiness/sponginess, cracking/splitting, hollowness, and internal browning. Different abiotic factors like moisture stress, temperature fluctuation, growing medium, nutrient imbalance, plant density and harvesting time cause a disturbance in the metabolic activities of root tissues that produce non-marketable roots. Therefore, this review provides a detail insight on the causes, physiology of these disorders, and the management practices to prevent them to produce commercial quality roots. This comprehensive knowledge will not only help the growers, but it will provide relative information for researchers as well to control these disorders through breeding innovations and biotechnological tools. Full article

Proverbs can be regarded as pithy axioms of traditional ecological knowledge (TEK), which can be useful for sustainability education. By using an exploratory sequential mixed method, we analyzed TEK and ecoliteracy components embedded in rain- and tree-related Korean traditional proverbs, and further conducted a survey of university students to gauge their knowledge of the proverbs and draw correlations with their ecoliteracy. We classified 173 rain- and 117 tree-related traditional Korean proverbs and found that most of these were related to factual observations, such as ecological characteristics of tree species and the prediction of rainfall, which indicated people’s understanding of ecological processes and patterns. We also found that students tended to provide correct answers for questions related to TEK-based worldviews, ethics and values, which are important components of ecoliteracy, thereby indicating the usefulness of proverbs in delivering TEK-based worldviews, ethics, and values. Overall, our study reasons that the learning of TEK-related traditional proverbs can be meaningful for enhancing ecoliteracy among urban youth, particularly when this learning is complemented with field-based observational learning within integrated approaches for sustainability education. Full article