Search Results (3)

Focusing on the challenges of low utilization of pineapple leaves and the poor stability of phenolic compounds (PCs) in pineapple leaf fiber using traditional solvent extraction methods, the effects of different extraction media (including distilled water, neutral methanol, acidic methanol, alkaline methanol, neutral ethanol, and alkaline ethanol) was systematically investigated on the extraction efficiency of PCs from pineapple leaf fiber. Through response surface methodology (RSM), the optimal extraction conditions were determined. Additionally, the impacts of illumination and pH on the stability of PCs in pineapple leaf fiber were thoroughly examined. The results demonstrated that acidic ethanol outperformed other extraction media, with the optimized extraction conditions as follows: ethanol concentration = 80%, material-to-liquid ratio = 1:40, extraction temperature = 70 °C, and extraction time = 40 min. Under these conditions, the extraction yield of PCs reached 11.55 mg/g. Furthermore, the stability of PCs was significantly enhanced by minimizing light exposure and maintaining a pH range of 3–6. The potential application of PCs extracted from pineapple leaf fiber was also explored, particularly in mango preservation, revealing promising results. This study not only provides an efficient and sustainable approach for the extraction and stabilization of PCs from pineapple leaf fiber but also opens new avenues for the utilization of polyphenols in functional applications, contributing to the valorization of agricultural by-products. Full article

Himantoglossum affine is a threatened terrestrial orchid. We aimed to optimize asymbiotic seed germination and direct embryogenesis and to analyze the phytochemical profile and physico-biochemical analysis of leaf and tuber. The individual use of organic nitrogen compounds resulted in higher germination efficiencies, while the shortest times to germination were observed using coconut water plus casein hydrolysate. Plantlets grown on media supplemented with pineapple juice and peptone had the highest plantlet length and weight. For embryogenesis, the highest regeneration rate (44%) and embryo number/explant (10.12 ± 2.08) were observed in young protocorm-like body (PLB) explants with 0.5 mg/L naphthalene acetic acid (NAA) and 1 mg/L thidiazuron (TDZ). During the acclimatization process, the scattered vascular tubes converted to fully developed vascular tissues, ensuring maximum sap flux. Gas chromatography–mass spectrometry analysis identified 1,2,3-propanetriol, monoacetate, 4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl, and 2-butenedioic acid, 2-methyl-, (E)- as the most prevalent compounds. We reported higher contents of total phenolics and flavonoids and antioxidant activity compared to other terrestrial orchids. The glucomannan content (36.96%) was also higher than starch content (31.31%), comparable to those reported in other tuberous orchids. Based on the fragmentation of H. affine populations in the Middle East and Euro-Mediterranean countries due to over-harvesting, climate change, and/or human impact, our procedure offers a tool for the re-introduction of in vitro-raised plants to threatened areas. Full article

Colitis is not fully curable, although currently, some treatment options are being adopted. In this study, we investigated the effects of pineapple leaf phenols (PLPs), natural phenol products from pineapple leaves, on DSS-induced colitis in mice. The results showed that PLPs dramatically decreased the inflammatory response by inhibiting NF-κB activation and the secretion of pro-inflammatory factors. Moreover, PLPs provided protection against DSS-induced acute colitis by maintaining epithelial integrity. Caffeic and P-coumaric acids had similar effects and could be the active components responsible for PLPs’ effect on colitis. These results indicate that the oral administration of PLPs might be considered as a therapeutic strategy in the treatment of patients with colitis. However, further research on clinical applications and the exact effect of PLPs on colitis is required. Full article