Search Results (100)

Optical clearing agents (OCAs) offer a promising approach to enhance skin transparency by reducing scattering and improving photon transmission, which is critical for non-invasive optical diagnostics such as glucose sensing and vascular imaging. However, the complex multilayered structure of skin and anatomical variability across different regions pose challenges for accurately evaluating OCA performance. In this study, we developed a multilayer Monte Carlo (MC) simulation model integrated with a depth- and time-resolved diffusion model based on Fick’s law to quantitatively assess the combined effects of OCA penetration depth and refractive index change on optical clearing. The model incorporates realistic skin parameters, including variable stratum corneum thicknesses, and was validated through in vivo experiments using glycerol and glucose at different concentrations. Both the simulation and experimental results demonstrate that increased stratum corneum thickness significantly reduces blood absorption of light and lowers the clearing efficiency of OCAs. The primary influence of stratum corneum thickness lies in requiring a greater degree of refractive index matching rather than necessitating a deeper OCA penetration depth to achieve effective optical clearing. These findings underscore the importance of considering regional skin differences when selecting OCAs and designing treatment protocols. This work provides quantitative insights into the interaction between tissue structure and optical response, supporting improved application strategies in clinical diagnostics. Full article

Optical sensor technologies are reshaping obesity detection by enabling non-invasive, dynamic analysis of biomechanical and morphological biomarkers. This review synthesizes recent advances in three key areas: optical gait analysis, vision-based pose estimation, and depth-sensing voxel modeling. Gait analysis leverages optical sensor arrays and video systems to identify obesity-specific deviations, such as reduced stride length and asymmetric movement patterns. Pose estimation algorithms—including markerless frameworks like OpenPose and MediaPipe—track kinematic patterns indicative of postural imbalance and altered locomotor control. Human voxel modeling reconstructs 3D body composition metrics, such as waist–hip ratio, through infrared-depth sensing, offering precise, contactless anthropometry. Despite their potential, challenges persist in sensor robustness under uncontrolled environments, algorithmic biases in diverse populations, and scalability for widespread deployment in existing health workflows. Emerging solutions such as federated learning and edge computing aim to address these limitations by enabling multimodal data harmonization and portable, real-time analytics. Future priorities involve standardizing validation protocols to ensure reproducibility, optimizing cost-efficacy for scalable deployment, and integrating optical systems with wearable technologies for holistic health monitoring. By shifting obesity diagnostics from static metrics to dynamic, multidimensional profiling, optical sensing paves the way for scalable public health interventions and personalized care strategies. Full article

We developed a photothermal deflection spectroscopy (PTDS) system for the noninvasive analysis of biological tissue. This system detects heat induced by irradiation with pulse-modulated mid-infrared light as the deflection of a probe laser. The probe light is incident on the sensing element horizontal with respect to its contact surface with the sample. This setup simplifies the optical alignment compared to conventional systems, which require the probe laser to be totally reflected at the prism contact surface and aligned with the point of mid-infrared light irradiation. In this study, we measured the PTDS spectra of biological samples to determine the characteristic features of their infrared absorption. We also compared the measurement reproducibility of two configurations: a horizontal optical path and a total reflection optical path. The horizontal optical path showed greater measurement reproducibility than the total reflection optical path when performing intermittent measurements on the wrist. Full article

This study proposes a novel heart rate estimation method that detects subtle cardiac-induced vibrations propagated through the cardiovascular system based on the ballistocardiography (BCG) principle, using a six-axis heart rate sensing device that integrates a three-axis accelerometer and a three-axis gyroscope. To validate the effectiveness of the proposed method, a comparative analysis was conducted against heart rate measurements obtained from photoplethysmography (PPG) sensors, which are widely used in conventional heart rate monitoring. Experiments were conducted on 20 adult participants, and frequency domain analysis was performed using different time windows of 30 s, 20 s, 8 s, and 4 s. The results showed that the 4 s window provided the highest accuracy in heart rate estimation, demonstrating that the proposed method can effectively capture fine cardiac-induced vibrations. This approach offers a significant advantage by utilizing inertial sensors commonly embedded in wearable devices for heart rate monitoring without the need for additional optical sensors. Compared to optical-based systems, the proposed method is more power-efficient and less affected by environmental factors such as ambient lighting conditions. The findings suggest that heart rate estimation using the six-axis heart rate sensing device presents a reliable, continuous, and non-invasive alternative for cardiovascular monitoring. Full article

This study investigates a noninvasive continuous glucose monitoring (NI-CGM) system optimized for earlobe application, leveraging the site’s anatomical advantages—absence of bone, muscle, and thick skin—for enhanced optical transmission. The system integrates multimodal sensing, combining near-infrared (NIR) diffuse transmission with temperature and pressure sensors. A novel Multi-Wavelength Slope Efficiency Near-Infrared Spectroscopy (MW-SE-NIRS) method is introduced, enhancing noise robustness through the slope efficiency-based parameterization of NIR signal dynamics. By employing three NIR wavelengths with distinct scattering and absorption properties, the method improves glucose detection reliability, addressing tissue heterogeneity and physiological noise in noninvasive monitoring. To validate the feasibility, a pilot clinical trial enrolled five participants with normal or pre-diabetic glucose profiles. Continuous glucose data capturing pre- and postprandial variations were analyzed using a 1D convolutional neural network (Conv1D). For three subjects under stable physiological conditions, the model achieved 97.0% Clarke error grid (CEG) A-Zone accuracy and a mean absolute relative difference (MARD) of 5.2%. Across all participants, results showed 90.9% CEG A-Zone accuracy and a MARD of 8.4%, with performance variations linked to individual factors such as earlobe thickness variability and physical activity. These outcomes demonstrate the potential of the MW-SE-NIRS system for noninvasive glucose monitoring and highlight the importance of future work on personalized modeling, sensor optimization, and larger-scale clinical validation. Full article

An optimized design of photodetectors and antennas for Förster Resonance Energy Transfer (FRET)-based glucose biosensing in endoscopic capsules is presented. The compact antenna design is tailored for the visible optical frequencies (~526 THz) associated with FRET-based glucose monitoring and integrates structural flexibility to conform to the spatial constraints of endoscopic capsules, such as mechanical bending features. The antenna is embedded in a multimode medium artificial tissue simulating a glucose environment with several layers, providing efficient coupling to the FRET emission signal for glucose sensing. Stable S11 parameters and a maximum gain of 9 dBi are realized by statelier mesh settings, bend adaptation, and cautious SAR constraint handlers. Results of the Specific Absorption Rate (SAR) confirm the limited energy absorption within permissible bounds, confirming its application for biomedical purposes. These results affirm the feasibility of non-invasive glucose measurement in interstitial fluid in this configuration that can be operable through an endoscope with improved sensitivity and functionality. Full article

Recently, sensing for biomolecules has become increasingly popular in the fields of environmental monitoring, personal health, and food safety. Plasmonic biosensors have been a powerful tool due to their high sensitivity and label-free operation. However, when it comes to molecules with different kinds and concentrations, detection technology and data processing remain a challenging task. In this study, we investigate the qualitative and quantitative detection of two kinds of biomolecules in the mid-infrared region simultaneously by the utilization of a plasmonic sensor. The strong coupling between each plasmonic resonance and the corresponding molecular vibration is found to significantly enhance the absorption signal of molecules, and the obtained Rabi splitting is not only a proof of molecular existence but also an indicator of molecular concentration. However, the amount of the molecular solution with a background refractive index in turn affects the plasmonic resonance position. In more general situations, it is not easy to achieve the match between plasmonic resonance and molecular resonance, and thus the quantitative detection by the Rabi splitting depth is not always feasible. Hence, we propose a machine learning algorithm called principal component analysis (PCA), providing a versatile approach for analyzing the proportion of each molecule in the mixture. Our work opens up new routes in noninvasive optical sensing and the integration of AI-driven data analysis further strengthens its potential for real-world applications. Full article

Water-quality monitoring in agricultural irrigation systems is challenging due to the dynamic and heterogeneous nature of mixed water sources, which complicates traditional and remote sensing-based assessment methods. Traditional water quality monitoring relies on water sampling and laboratory analysis, which can be time-consuming, labor-intensive, and spatially limited. In situ hyperspectral reflectance sensing (HRS) presents a promising alternative, offering high-resolution, non-invasive monitoring capabilities. However, applying HRS in mixed-water environments—where served-water effluent, precipitation, and natural river water converge—presents significant challenges due to variability in water composition and environmental conditions. While HRS has been widely explored in controlled or homogeneous water bodies, its application in highly dynamic agricultural mixed-water systems remains understudied. This study addresses this gap by evaluating the relationships between in situ hyperspectral data (450–900 nm) and key water-quality parameters—pH, turbidity, nitrates, and chlorophyll-a—across three campaigns in a Colombian tropical agricultural irrigation system. A Pearson’s correlation analysis revealed the strongest spectral associations for nitrates, with positive correlations at 500 nm (r ≈ 0.76) and 700 nm (r ≈ 0.85) and negative correlations in the near-infrared (850 nm, r ≈ −0.88). Conversely, the pH exhibited weak and diffuse correlations, with a maximum of r ≈ 0.51. Despite their optical activity, turbidity and chlorophyll-a showed unexpectedly weak correlations, likely due to the optical complexity of the mixed water matrix. Random Forest regression identified key spectral regions for each parameter, yet model performance was limited, with R² values ranging from 0.51 (pH) to −1.30 (chlorophyll-a), and RMSE values between 0.41 and 1.51, reflecting the challenges of predictive modeling in spatially and temporally heterogeneous wastewater systems. Despite these challenges, this study establishes a baseline for future hyperspectral applications in complex agricultural water monitoring and highlights critical spectral regions for further investigation. To improve the feasibility of HRS in mixed-water assessments, future research should focus on enhancing data-preprocessing techniques, integrating complementary sensing modalities, and refining predictive models to better account for environmental variability. Full article

Fiber-optic Fabry–Pérot (F–P) sensors offer significant potential for non-invasive hemodynamic monitoring, but existing sensing systems face limitations in multi-channel measurement capabilities and dynamic demodulation accuracy. This study introduces a sparse-frequency-scanning white-light interferometry (SFS-WLI) system with an adaptive mode-locked cross-correlation (MLCC) algorithm to address these challenges. The system leverages telecom-grade semiconductor lasers (191.2–196.15 THz sweep range, 50 GHz step) and a Fibonacci-optimized MLCC algorithm to achieve real-time cavity length demodulation at 5 kHz. Compared to normal MLCC algorithm, the Fibonacci-optimized algorithm reduces the number of computational iterations by 57 times while maintaining sub-nanometer resolution under dynamic perturbations. Experimental validation demonstrated a carotid–radial pulse wave velocity of 5.12 m/s in a healthy male volunteer. This work provides a scalable and cost-effective solution for cardiovascular monitoring with potential applications in point-of-care testing (POCT) and telemedicine. Full article

The phase-sensitive optical time domain reflectometry (Φ-OTDR) technique offers a method for distributed acoustic sensing (DAS) systems to detect external acoustic fluctuations and mechanical vibrations. By accurately identifying vibration events, DAS systems provide a non-invasive solution for security monitoring. However, limitations in temporal signal analysis and the lack of spatial features significantly impact classification accuracy in event recognition. To address these challenges, this paper proposes a network model for vibration-event recognition that integrates convolutional neural networks (CNNs), bidirectional gated recurrent units (BiGRUs), and attention mechanisms, referred to as CNN-BiGRU-Attention (CBA). First, the CBA model processes spatiotemporal matrices converted from raw signals, extracting low-level features through convolution and pooling. Subsequently, features are further extracted and separated along both the temporal and spatial dimensions. In the spatial-dimension branch, horizontal convolution and pooling generate enhanced spatial feature maps. In the temporal-dimension branch, vertical convolution and pooling are followed by BiGRU processing to capture dynamic changes in vibration events from both past and future contexts. Additionally, the attention mechanism focuses on extracted features in both dimensions. The features from the two dimensions are then fused using two cross-attention mechanisms. Finally, classification probabilities are output through a fully connected layer and a softmax activation function. In the experimental simulation section, the model is validated using real-world data. A comparison with four other typical models demonstrates that the proposed CBA model offers significant advantages in both recognition accuracy and robustness. Full article

Achieving both accurate and real-time monitoring heartbeat signals by non-invasive sensing techniques is challenging due to various noise interferences. In this paper, we propose an enhanced discrete wavelet transform (DWT) method that incorporates objective denoising quality assessment metrics to determine accurate thresholds and adaptive threshold functions. Our approach begins by denoising ECG signals from various databases, introducing several types of typical noise, including additive white Gaussian (AWG) noise, baseline wandering noise, electrode motion noise, and muscle artifacts. The results show that for Gaussian white noise denoising, the enhanced DWT can achieve 1–5 dB SNR improvement compared to the traditional DWT method, while for real noise denoising, our proposed method improves the SNR tens or even hundreds of times that of the state-of-the-art denoising techniques. Furthermore, we validate the effectiveness of the enhanced DWT method by visualizing and comparing the denoising results of heartbeat signals monitored by fiber-optic micro-vibration sensors against those obtained using other denoising methods. The improved DWT enhances the quality of heartbeat signals from non-invasive sensors, thereby increasing the accuracy of cardiovascular disease diagnosis. Full article

Accurate in vivo glucose monitoring is essential for effective diabetes management and for the care of pre-term infants in critical care. Glucose-monitoring techniques are broadly categorized into three types: invasive, minimally invasive, and non-invasive. Each method presents distinct advantages and challenges. Non-invasive glucose sensors, despite impressive advancements in recent years, still face issues with signal interference and accuracy, limiting their widespread clinical application. In contrast, implanted devices offer more reliable and consistent results in clinical settings, making them the current gold standard. This review provides an overview of the leading glucose-sensing technologies, detailing both their advantages and drawbacks. We discuss invasive techniques, such as implanted electrodes, which allow continuous glucose monitoring with high accuracy, but often come with risks of infection and discomfort. Minimally invasive methods, such as fluorescence sensors, Raman sensors, and microneedle arrays, aim to reduce discomfort while providing more precise measurements than non-invasive devices. Additionally, non-invasive methods, such as optical, infrared, and microwave techniques, are explored for their potential to provide pain-free, continuous glucose monitoring. Finally, the review highlights a brief comparison among the current technologies and future directions in the field, particularly the use of signal enhancement algorithms and integration with wearable devices. Full article

The sensory quality of meat, encompassing the traits of appearance, texture, and flavour, is essential to consumer acceptance. Conventional quality assessment techniques, such as instrumental methods and trained sensory panels, often face limitations due to their destructive and time-consuming nature. In recent years, optical sensing techniques have emerged as a fast, non-invasive, and non-destructive technique for the prediction of quality attributes in meat and meat products, achieving prediction accuracies of over 90%. This review critically examines the potential of optical sensing techniques, such as near-infrared spectroscopy (NIRS), Raman spectroscopy, and hyperspectral imaging (HSI), to inform about the sensory attributes of red meat, aligning with industrial demands for early information on the predicted sensory performance of inventory to support meeting consumer requirements. Recent trends and the remaining challenges associated with these techniques will be described. While technical issues related to spectral data acquisition and data processing are important challenges when considering industrial implementation, overall, optical sensing techniques, in tandem with recent developments in digitalisation and data analytics, provide potential for the online prediction of meat sensory quality in the meat processing industries. Establishing technologies for enhanced information on the product and improved possibilities for quality control will help the industry to meet consumer demands for a consistent quality of product. Full article

Polarization optics, which characterize the orientation of the electromagnetic field through Stokes vectors formalism, have been effectively used in lidar remote sensing to detect particles that differ in shape, such as mineral dust or pollen. In this study, for the first time, we explore the capability of polarization optics to distinguish the light-backscattering patterns of pollen and fungal spores, two complex-shaped particles that vary significantly in surface structure. A unique laboratory polarimeter operating at lidar backscattering at 180.0° was conducted to assess their light depolarization property in laboratory ambient air. If, at the precise lidar backscattering angle of 180.0°, the depolarization ratios of pollen and fungal spores were difficult to differentiate, slight deviations from 180.0° allowed us to reveal separate scattering matrices for pollen and fungal spores. This demonstrates that polarization optics can unambiguously differentiate these particles based on their light-(back)scattering properties. These findings are consistent at both 532 and 1064 nm. This non-invasive, real-time technique is valuable for environmental monitoring, where rapid identification of airborne allergens is essential, as well as in agricultural and health sectors. Polarization-based light scattering thus offers a valuable method for characterizing such atmospheric particles, aiding in managing airborne contaminants. Full article

Transcutaneous carbon dioxide (TcPCO2) monitoring provides a non-invasive alternative to measuring arterial carbon dioxide (PaCO2), making it valuable for various applications, such as sleep diagnostics and neonatal care. However, traditional transcutaneous monitors are bulky, expensive, and pose risks such as skin burns. To address these limitations, we have introduced a compact, cost-effective CMOS imager-based sensor for TcPCO2 detection by utilizing colorimetric reactions with metal–organic framework (MOF)-based nano-hybrid materials. The sensor, with a colorimetric sensing array fabricated on an ultrathin PDMS membrane and then adhered to the CMOS imager surface, can record real-time sensing data through image processing without the need for additional optical components, which significantly reduces the sensor’s size. Our system shows impressive sensitivity and selectivity, with a low detection limit of 26 ppm, a broad detection range of 0–2% CO₂, and strong resistance to interference from common skin gases. Feasibility tests on human subjects demonstrate the potential of this MOF-CMOS imager-based colorimetric sensor for clinical applications. Additionally, its compact design and responsiveness make it suitable for sports and exercise settings, offering valuable insights into respiratory function and performance. The sensing system’s compact size, low cost, and reversible and highly sensitive TcPCO2 monitoring capability make it ideal for integration into wearable devices for remote health tracking. Full article