Search Results (37)

Nanoparticles have emerged as innovative tools for combating bacterial infections, offering a potential solution to antibiotic resistance and the limitations of conventional antimicrobials. Nanoparticles exhibit antibacterial activity through multiple mechanisms, including oxidative stress induction, metal ion release, direct membrane damage, disruption of DNA and proteins, and indirect immune system enhancement. Rickettsia helvetica, R. monacensis, R. slovaca, and R. conorii subsp. raoultii are tick-borne pathogens transmitted by Ixodes ricinus, Dermacentor reticulatus, and D. marginatus ticks across Europe causing spotted fever rickettsiosis. While rickettsioses are successfully treated with antibiotics, resistance of rickettsiae to antimicrobial therapy has been reported. Here, we evaluated the anti-rickettsial activity of silver (AgNPs), selenium (SeNPs), and chitosan (CSNPs) nanoparticles against R. conorii subsp. caspia, a tick-borne bacterial pathogen, in African green monkey kidney cell line (Vero). At their highest non-cytotoxic concentrations, CSNPs exhibited the strongest inhibitory effect (87%). SeNPs also significantly reduced bacterial load (76%), although their efficacy was constrained by cytotoxicity at higher doses. In contrast, AgNPs did not show significant activity under the tested conditions. The differences observed among nanoparticles reflect both the antimicrobial properties and host cell tolerance limits. These findings highlight CSNPs and SeNPs as promising candidates for further development of nanoparticle-based strategies to combat intracellular, tick-borne pathogens. Full article

In recent years, China has actively advanced the new energy vehicle industry to achieve its “dual carbon” objectives via a green revolution. The growth of green technical innovation by new energy vehicle enterprises has emerged as a significant national support project, and it has implemented a number of new energy vehicle promotion policies. Therefore, it is essential to investigate if promotional policies encourage the development of green technologies in businesses. China’s 2016 “New Energy Vehicle Promotion Catalogue” serves as the policy’s temporal shock point, and data from Chinese-listed new energy vehicle companies from 2011 to 2022 are used in this study. The effect and mechanism of the new energy vehicle promotion strategy on developing green technologies in businesses are investigated using a double difference model. As per the research, the promotion policy substantially enhances the green technological innovation of new energy vehicle firms. It can augment the level of R&D investment and alleviate financing constraints for enterprises, and enterprises’ social responsibility can act as a positive moderator for the promotion policy and enterprise green technological innovation. Finally, it has a more apparent positive impact on the green technological innovation of major companies and non-state-owned enterprises compared to state-owned firms. Additionally, it is more evident that enterprises are raising green technology innovation in the eastern and central regions. Full article

When the protective mechanisms of the gastroesophageal mucosa are overwhelmed by injurious factors, the structural and functional mucosal integrity is compromised, resulting in a wide spectrum of disorders. Poliprotect has recently been shown to be non-inferior to standard-dose omeprazole for the treatment of endoscopy-negative patients with heartburn and/or epigastric pain or burning. Here, we provide preclinical data describing the mechanism of action of the Poliprotect formulation, a 100% natural, biodegradable, and environmental friendly medical device according to EU 2017/745 and containing UVCB (unknown or variable composition, complex-reaction products, or biological materials) substances of botanical and mineral origin, according to the REACH and European Chemical Agency definitions. Different in vitro assays demonstrated the capability of Poliprotect to adhere to mucus-secreting gastric cells and concomitantly deliver a local barrier with buffering and antioxidant activity. In studies conducted in accordance with systems biology principles, we evaluated the effects of this barrier on human gastric cells exposed to acidic stress. Biological functions identified via Ingenuity Pathway Analysis highlighted the product’s ability to create a microenvironment that supports the mucosal structural and functional integrity, promotes healing, and restores a balanced mucosal inflammatory status. Additionally, transepithelial electrical resistance and an Ussing chamber showed the product’s capability of preserving the integrity of the gastric and esophageal epithelial barriers when exposed to an acid solution. Two in vivo models of erosive gastropathy further highlighted its topical protection against ethanol- and drug-induced mucosal injury. Overall, our findings sustain the feasibility of a paradigm shift in therapeutics R&D by depicting a very innovative and desirable mode of interaction with the human body based on the emerging biophysical, rather than the pharmacological properties of these therapeutic agents. Full article

The imperative for sustainable development demands innovative solutions to address the environmental impact of transportation, particularly in the context of climate change. This study explores the intersection of renewable energy and sustainability within the automotive industry by examining how restrictions on the sale of gasoline-powered vehicles affect patent filings related to automotive engine technologies. Our approach employs a factorial analysis to capture structural shifts in patent filings, utilizing the logarithmic mean Divisia index for index decomposition. We scrutinize patent trends from 1985 to 2019, with a focus on both non-green (internal combustion engine vehicles) and green technologies (battery electric, hybrid electric, and full cell vehicles), to assess the transition towards sustainable transportation. This study also scrutinizes the patenting activities of four major patent offices—China, Japan, the United States, and Germany—to unveil global trends and disparities in sustainable technology innovation. Our findings underscore how a nation’s green investment strategy is contingent upon its developmental stage, with intellectual property rights and R&D incentives playing pivotal roles in shaping R&D landscapes, especially in emerging economies with nascent intellectual property markets. This study also reveals varying strategies for developing green automotive engines across nations, indicating that the growth of green patents in developed countries is probably spurred by financial incentives and enhanced intellectual property rights to promote specific tech advancements. This research contributes to the discourse on sustainability by highlighting the critical role of policy in fostering green technology development and the importance of aligning patent strategies with environmental goals. Full article

The Sustainable Development Goals 9 (known as the SDG9): Industry, Innovation, and Infrastructure are crucial objectives for Kuwait since they are essential to the nation’s attempts to achieve sustainable development and economic diversification. Using the recently available literature, this research attempts to understand and investigate the current level of SDG9 implementation in the context of Kuwait. Data from the SDGs database based on published studies and reports were used in this study. The actual level of SDG9 implementation in Kuwait still faces significant challenges. The roadmap to achieving the SDG9 goals was improved based on the analysis of SDG9 targets and identifying the reasons and factors for developing each target. The results indicate that Kuwait should encourage non-oil businesses, including manufacturing and renewable energy, to diversify its economy and reduce its dependency on oil. Efforts need to be taken to improve industrial infrastructure, encourage innovation, and advance research and development (R&D) activities. To attract capital and skills for infrastructure development, Kuwait should also promote public–private partnerships. In order to achieve the SDG9 goals, it is suggested that policymakers foster innovation and technology adoption, support small and medium-sized businesses (SMEs), promote sustainable industrial practices, build industrial infrastructure, improve vocational and technical education, encourage international cooperation and technology transfer, monitor progress, and set targets. Full article

In order to address the climate change crisis, China has actively promoted low-carbon urban development. China has implemented a policy known as the low-carbon city pilot policy (LCCPP). Given the enterprise life cycle theory, a multi-period DID model is employed to investigate the influence of the LCCPP on enterprise’s green technology innovation (GTI). The research reveals that the LCCPP can actually promote enterprise’s GTI. The LCCPP significantly stimulates GTI of enterprises in the growth phase, but not for enterprises in the maturity and recession phases. Path analysis indicates that the LCCPP can enhance enterprise’s GTI by increasing government fiscal subsidies to and research and development (R&D) of firms. Further analysis reveals that although the GTI effects of the LCCPS are not effective for state-owned enterprises (SOEs) and non-manufacturing businesses, it is significant for those enterprises that are in the growth stage. The LCCPP successfully enhances substantial innovation within enterprises, but not for strategic innovation within enterprises. This study enriches the existing scholarly research on the influence of the LCCPP on enterprise’s GTI and holds practical implications for promoting accurate and effective GTI among enterprises, thereby further advancing the transition towards low-carbon urban development. Full article

The independent innovation of agricultural enterprises ensures national food security through enhancing food production efficiency and optimizing food nutritional quality. Independent innovation is an important way for the sustainable growth of agricultural enterprises. However, in this process, there are issues such as long cycles and high risks. Based on this, using the panel data of China’s listed agricultural enterprises from 2007 to 2021, this study empirically examined the impact of independent innovation on the sustainable growth of agricultural enterprises, as well as the moderating effects of internal drivers (digital transformation) and external pull factors (government subsidies) through a two-way fixed effects model. The findings are the following: (1) The impact of independent innovation on the sustainable growth of agricultural enterprises exhibits an “inverted U-pattern”. When the R&D investment of agricultural enterprises accounts for 77.85% of operating income, the sustainable growth ability of agricultural enterprises is the highest. (2) Compared with large agricultural enterprises, small and medium-sized agricultural enterprises reach the threshold of independent innovation ability later, and the incentive effect range of independent innovation is longer. (3) Independent innovation exerts a more significant “inverted U” effect on the sustainable growth of non-state-owned agricultural enterprises and agricultural enterprises in the mature stage, while its impact on the sustainable growth of state-owned agricultural enterprises and agricultural enterprises in the growth and decline stages is not significant. (4) Government subsidies can help enhance the positive impact of independent innovation on the sustainable growth of agricultural enterprises when it does not exceed the threshold but cannot alleviate the negative impact of independent innovation on the sustainable growth of agricultural enterprises when it exceeds the threshold; investment in digital transformation not only helps to enhance the positive impact of independent innovation on the sustainable growth of agricultural enterprises when it does not exceed the threshold but also helps to alleviate the negative impact of independent innovation on the sustainable growth of agricultural enterprises when it exceeds the threshold. The research results provide data support for agricultural enterprises to carry out innovation activities under internal drive and external pull. At the same time, it is of great significance for the national implementation of the strategy of storing food in technology and food security strategy. Full article

Lung cancer is the leading cause of cancer-related death worldwide. Non-coding RNAs are emerging as critical players for the onset and progression of cancer. Analyses of three different datasets revealed that the lncRNA JPX was overexpressed in adenocarcinoma tissues in comparison to normal lungs, as expected for an oncogene. Intriguingly, the predicted binding miR-378a-3p showed a significant inverse correlation with JPX expression. The lncRNA/miRNA physical interaction was validated by reporter vectors. Then, the oncogenic activity of JPX, the tumor-suppressive role of miR-378a-3p, and the contribution of their functional interaction to cancer hallmarks were demonstrated using assays for cell proliferation, migration, invasion, and 3D-spheroid formation. Finally, molecular circuits were investigated by boosting the expression of both JPX and miR-378a-3p, singularly and in combination, demonstrating that JPX counteracted miR-378a-3p silencing activity toward its oncogenic targets GLUT1, NRP1, YY1, and Wnt5a. Overall, the data unveil a novel ceRNET (competing endogenous RNA network), wherein JPX acts as a ceRNA by binding to miR-378a-3p, thus reducing the miRNA silencing activity toward its downstream targets, and eliciting oncogenic pathways driving lung cancer. The knowledge of the network may pave the way to develop new diagnostic panels, and innovative RNA-targeted and RNA-based therapeutic strategies. Full article

This research paper examines the spillover effect of ESG performance on green innovation behavior in companies within the same industry. The study specifically focuses on listed companies on the Shanghai Stock Exchange and Shenzhen Stock Exchange between 2011 and 2020. The results indicate that peer firms with superior environmental, social, and governance (ESG) performance have a notable and beneficial impact on the green innovation activities, quantities, and qualities of their counterparts. Significantly, this phenomenon is especially evident for the ecological (E) and societal (S) aspects of ESG performance when considering companies within the same industry. Additionally, according to our analysis, the association between peer firms’ improved ESG performance and subsequent gains in green innovation activities is mediated by higher R&D expenditure and increased green consciousness. The robustness of these findings persists even after resolving issues of endogeneity through thorough testing. In addition, this paper finds that the spillover effects are more significant for non-state-owned firms, small-sized firms, firms with more analyst attention, firms in non-highly polluting industries, and when external environmental regulations are stronger. Full article

According to their resource ownership and resource acquisition channels, this paper constructs an evolutionary game model of enterprises’ technological innovation investment channels considering managers’ expectations. This results in the strategy of non-alliance firms along the first channel having a significant impact on the evolutionary equilibrium. For non-alliance enterprises, the difference in the amount of investment in the R&D stage has the same impact on the probability of such enterprises following the first channel. The number of active managers, group capacity, the difference in managers’ expectations, and the difference in the amount of technological innovation investment in the R&D stage have significant differences in the possibility of choosing the first channel between the two types of enterprises. The research conclusions can provide valuable references for enterprises to make technological innovation decisions. Full article

Acne-prone skin is associated with dysbiosis involving Cutibacterium acnes (C. acnes) and Staphylococcus epidermidis (S. epidermidis) causing increased seborrhea in sebaceous glands (SG) and inflammation. Human primary sebocytes were cultivated using 1.10⁶ UFC/mL C. acnes Type IA (facial acne, ATCC6919) and/or 1.10⁵ UFC/mL S. epidermidis (unknown origin, ATCC12228) for 48 h in our SEB4GLN-optimized media without antibiotics. Bacteria and sebocytes were enumerated and assessed to determine their viability. Lipid production was imaged and quantified via Nile Red staining. SG with hair follicles were microdissected from healthy skin and cultured using 1.10⁵ UFC/mL C. acnes Type 1A and/or 1.10⁴ UFC/mL S. epidermidis (wild-type facial skin strain) through prior fixation and immunostaining for MC5R, C. acnes and nuclei (DAPI) via Z-stack confocal microscopy bioimaging (Leica SP5X & FIJI software, Version 2.9.0). C. acnes growth was not impacted when co-cultivated with sebocytes (2D) or SG (3D) models. Phylotype IA stimulated sebocyte lipid production, which had no impact on viability. The S. epidermidis reference strain overproliferated, inducing sebocyte mortality. For 3D SG model, culture conditions were optimized using a wild-type facial skin strain at a lower concentration, 1:10 ratio to C. acnes, reduced contact time, sequential inoculation and rinsing step. Bioimaging revealed strong C. acnes labeling in the active areas of the pilosebaceous unit. S. epidermidis formed biofilm, which was distributed across the SG via non-specific fluorescence imaging. We developed an innovative model of a sebaceous gland that mimics acne-prone skin with lipid overproduction and virulent phylotype IA C. acnes inoculation. Full article

Glioblastoma (GBM) is the most common primary brain cancer in adults. Despite the remarkable advancements in recent years in the realm of cancer diagnosis and therapy, regrettably, GBM remains the most lethal form of brain cancer. In this view, the fascinating area of nanotechnology has emerged as an innovative strategy for developing novel nanomaterials for cancer nanomedicine, such as artificial enzymes, termed nanozymes, with intrinsic enzyme-like activities. Therefore, this study reports for the first time the design, synthesis, and extensive characterization of innovative colloidal nanostructures made of cobalt-doped iron oxide nanoparticles chemically stabilized by a carboxymethylcellulose capping ligand (i.e., Co-MION), creating a peroxidase-like (POD) nanozyme for biocatalytically killing GBM cancer cells. These nanoconjugates were produced using a strictly green aqueous process under mild conditions to create non-toxic bioengineered nanotherapeutics against GBM cells. The nanozyme (Co-MION) showed a magnetite inorganic crystalline core with a uniform spherical morphology (diameter, 2R = 6–7 nm) stabilized by the CMC biopolymer, producing a hydrodynamic diameter (H_D) of 41–52 nm and a negatively charged surface (ZP~−50 mV). Thus, we created supramolecular water-dispersible colloidal nanostructures composed of an inorganic core (Cox-MION) and a surrounding biopolymer shell (CMC). The nanozymes confirmed the cytotoxicity evaluated by an MTT bioassay using a 2D culture in vitro of U87 brain cancer cells, which was concentration-dependent and boosted by increasing the cobalt-doping content in the nanosystems. Additionally, the results confirmed that the lethality of U87 brain cancer cells was predominantly caused by the production of toxic cell-damaging reactive oxygen species (ROS) through the in situ generation of hydroxyl radicals (·OH) by the peroxidase-like activity displayed by nanozymes. Thus, the nanozymes induced apoptosis (i.e., programmed cell death) and ferroptosis (i.e., lipid peroxidation) pathways by intracellular biocatalytic enzyme-like activity. More importantly, based on the 3D spheroids model, these nanozymes inhibited tumor growth and remarkably reduced the malignant tumor volume after the nanotherapeutic treatment (ΔV~40%). The kinetics of the anticancer activity of these novel nanotherapeutic agents decreased with the time of incubation of the GBM 3D models, indicating a similar trend commonly observed in tumor microenvironments (TMEs). Furthermore, the results demonstrated that the 2D in vitro model overestimated the relative efficiency of the anticancer agents (i.e., nanozymes and the DOX drug) compared to the 3D spheroid models. These findings are notable as they evidenced that the 3D spheroid model resembles more precisely the TME of “real” brain cancer tumors in patients than 2D cell cultures. Thus, based on our groundwork, 3D tumor spheroid models might be able to offer transitional systems between conventional 2D cell cultures and complex biological in vivo models for evaluating anticancer agents more precisely. These nanotherapeutics offer a wide avenue of opportunities to develop innovative nanomedicines for fighting against cancerous tumors and reducing the frequency of severe side effects in conventionally applied chemotherapy-based treatments. Full article

Mycobacteria form some of the most notorious and difficult-to-treat bacterial pathogens. As a group, they are intrinsically resistant to many commonly used antibiotics, such as tetracyclines and beta-lactams. In addition to intrinsic resistances, acquired multidrug resistance has also been observed and documented in Mycobacterium tuberculosis (MTB), Mycobacterium leprae and non-tuberculous mycobacteria (NTM). To combat multidrug resistant infections by these pathogens, innovative antimicrobials and treatment regimens are required. In this regard, linezolid, an oxazolidinone introduced for clinical use just two decades ago, was added to the therapeutic armamentarium for drug-resistant mycobacteria. It exhibits antibacterial activity by binding to the 50S ribosomal subunit and inhibiting protein synthesis. Unfortunately, linezolid resistance has now been documented in MTB and NTM, in many parts of the world. Most linezolid-resistant mycobacterial strains show mutations in the ribosome or related genes, such as in the rplC, rrl and tsnR genes. Non-ribosomal mechanisms appear to be rare. One such mechanism was associated with a mutation in fadD32, which encodes a protein that plays an important role in mycolic acid synthesis. Mycobacterial efflux proteins have also been implicated in linezolid resistance. This review summarises current knowledge of genetic determinants of linezolid resistance in mycobacteria, with the aim of contributing information that could facilitate the discovery of new therapeutic approaches to overcome, delay or avoid further developments of drug resistance among these important pathogens. Full article

Oil and gas (O&G) companies are facing increasing pressure to transform their businesses in order to contribute effectively to the transition from an economy based on fossil fuels to one driven by clean energy technologies. Understanding how O&G companies can define actions to comply with the United Nations’ Sustainable Development Goals (SDGs) and, specifically, how they can generate a positive impact in terms of sustainability through technology innovation becomes relevant in order to guarantee the success of such transformation. To explore this issue, this article analyzes, using a case study research methodology, the sustainability strategy of an O&G company in the Basque Country region (Spain) that is undergoing a profound transformation of its overall business strategy. In particular, the analysis focuses on how the company’s innovation and research and development (R&D) activities and projects related to clean technologies contribute to fulfilling the energy- and non-energy-related SDGs. The main result of the analysis is the identification and characterization of an emerging complex public–private multi-stakeholder business and innovation ecosystem surrounding the O&G company under scrutiny with a clear focus on low-carbon technologies. This ecosystem channels knowledge and innovation synergies and spillovers at the local and regional levels, encouraging green industrial growth in different value chains, and provides insights about how O&G companies can contribute effectively to the SDGs and, at the same time, increase the sustainability of their businesses. Full article

A firm’s sustainable technological innovation (STI) is an important strategy to cope with the global challenges of the climate emergency and resource constraints. To encourage firms to pursue sustainable innovation, the government put its efforts into designing a proper environmental policy (EP). According to Porter’s hypothesis, a well-designed and flexible EP will advance the pace of a firm’s innovation. This paper argues that a flexible EP portfolio combining market and non-market-based EP instruments may affect a firm’s STI. Market-based EP instruments are cost-effective and consistent from a long-term view, whereas non-market-based EP instruments are more forceful and effective in the short term. Hence, these two kinds of EP instruments could complement each other. Furthermore, technical executives in top management teams will moderate the relationships between EP instruments and firms’ STI. Data analysis results of 618 Chinese public firms, who constantly participated in R&D activities during 2015–2019, supported these hypotheses. Contributions to EP and firm innovation theory, as well as suggestions for policymakers and firms’ top management teams, are discussed. Full article