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Keywords = immobilized G. oxydans

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11 pages, 2811 KiB  
Article
Use of PEDOT:PSS/Graphene/Nafion Composite in Biosensors Based on Acetic Acid Bacteria
by Yulia Plekhanova, Sergei Tarasov and Anatoly Reshetilov
Biosensors 2021, 11(9), 332; https://doi.org/10.3390/bios11090332 - 13 Sep 2021
Cited by 18 | Viewed by 4022
Abstract
Immobilization of the biocomponent is one of the most important stages in the development of microbial biosensors. In this study, we examined the electrochemical properties of a novel PEDOT:PSS/graphene/Nafion composite used to immobilize Gluconobacter oxydans bacterial cells on the surface of a graphite [...] Read more.
Immobilization of the biocomponent is one of the most important stages in the development of microbial biosensors. In this study, we examined the electrochemical properties of a novel PEDOT:PSS/graphene/Nafion composite used to immobilize Gluconobacter oxydans bacterial cells on the surface of a graphite screen-printed electrode. Bioelectrode responses to glucose in the presence of a redox mediator 2,6-dichlorophenolindophenol were studied. The presence of graphene in the composite reduced the negative effect of PEDOT:PSS on cells and improved its conductivity. The use of Nafion enabled maintaining the activity of acetic acid bacteria at the original level for 120 days. The sensitivity of the bioelectrode based on G. oxydans/PEDOT:PSS/graphene/Nafion composite was shown to be 22 μA × mM−1 × cm−2 within the linear range of glucose concentrations. The developed composite can be used both in designing bioelectrochemical microbial devices and in biotechnology productions for long-term immobilization of microorganisms. Full article
(This article belongs to the Special Issue Cell Based Biosensors)
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16 pages, 4469 KiB  
Article
Valorization of Waste Glycerol to Dihydroxyacetone with Biocatalysts Obtained from Gluconobacter oxydans
by Lidia Stasiak-Różańska, Anna Berthold-Pluta and Pritam Kumar Dikshit
Appl. Sci. 2018, 8(12), 2517; https://doi.org/10.3390/app8122517 - 6 Dec 2018
Cited by 16 | Viewed by 7238
Abstract
Waste glycerol is the main by-product generated during biodiesel production, in an amount reaching up to 10% of the produced biofuel. Is there any method which allows changing this waste into industrial valuable compounds? This manuscript describes a method for valorization of crude [...] Read more.
Waste glycerol is the main by-product generated during biodiesel production, in an amount reaching up to 10% of the produced biofuel. Is there any method which allows changing this waste into industrial valuable compounds? This manuscript describes a method for valorization of crude glycerol via microbial bioconversion. It has been shown that the use of free and immobilized biocatalysts obtained from Gluconobacter oxydans can enable beneficial valorization of crude glycerol to industrially valuable dihydroxyacetone. The highest concentration of this compound, reaching over 20 g·L−1, was obtained after 72 h of biotransformation with free G. oxydans cells, in a medium containing 30 or 50 g·L−1 of waste glycerol. Using a free cell extract resulted in higher concentrations of dihydroxyacetone and a higher valorization efficiency (up to 98%) compared to the reaction with an immobilized cell extract. Increasing waste glycerol concentration to 50 g·L−1 causes neither a faster nor higher increase in product yield and reaction efficiency compared to its initial concentration of 30 g·L−1. The proposed method could be an alternative for utilization of a petrochemical waste into industry applicated chemicals. Full article
(This article belongs to the Special Issue Hazardous Waste Treatment)
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17 pages, 717 KiB  
Article
Ink-Jet Printing of Gluconobacter oxydans: Micropatterned Coatings As High Surface-to-Volume Ratio Bio-Reactive Coatings
by Marcello Fidaleo, Nadia Bortone, Mark Schulte and Michael C. Flickinger
Coatings 2014, 4(1), 1-17; https://doi.org/10.3390/coatings4010001 - 19 Dec 2013
Cited by 10 | Viewed by 7742
Abstract
We formulated a latex ink for ink-jet deposition of viable Gram-negative bacterium Gluconobacter oxydans as a model adhesive, thin, highly bio-reactive microstructured microbial coating. Control of G. oxydans latex-based ink viscosity by dilution with water allowed ink-jet piezoelectric droplet deposition of 30 × [...] Read more.
We formulated a latex ink for ink-jet deposition of viable Gram-negative bacterium Gluconobacter oxydans as a model adhesive, thin, highly bio-reactive microstructured microbial coating. Control of G. oxydans latex-based ink viscosity by dilution with water allowed ink-jet piezoelectric droplet deposition of 30 × 30 arrays of two or three droplets/dot microstructures on a polyester substrate. Profilometry analysis was used to study the resulting dry microstructures. Arrays of individual dots with base diameters of ~233–241 µm were obtained. Ring-shaped dots with dot edges higher than the center, 2.2 and 0.9 µm respectively, were obtained when a one-to-four diluted ink was used. With a less diluted ink (one-to-two diluted), the microstructure became more uniform with an average height of 3.0 µm, but the ink-jet printability was more difficult. Reactivity of the ink-jet deposited microstructures following drying and rehydration was studied in a non-growth medium by oxidation of 50 g/L D-sorbitol to L-sorbose, and a high dot volumetric reaction rate was measured (~435 g·L−1·h−1). These results indicate that latex ink microstructures generated by ink-jet printing may hold considerable potential for 3D fabrication of high surface-to-volume ratio biocoatings for use as microbial biosensors with the aim of coating microbes as reactive biosensors on electronic devices and circuit chips. Full article
(This article belongs to the Special Issue Advancing Coatings with Biotechnology)
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