Search Results (24)

In this study, for first time, the efficiency of the yield and fruit quality of the pink tomato cultivar ‘Tomimaru Muchoo’ was studied in hydroponic cultivation with top and interlighting with LEDs (light-emitting diodes) and diffusion glass (DGlass) to cover the glasshouse roof (LED+LED+DGlass). DGlass was also tested in tomato cultivation with only top lighting by sodium lamps (HPS+DGlass). The control, which had no DGlass (HPS, high-pressure sodium), was a crop with sodium lamps from above, where the roof was covered with conventional glasshouse glass. Selected parameters of tomato plant growth and development, photosynthetic activity, and chemical composition were analysed. Tomato fruits were evaluated for their physical and chemical characteristics as well as sensory quality. LED+LED+DGlass-supplemented tomato plants had the shortest fruiting stems, a higher dry matter of leaves, higher Mg²⁺ ion concentration, and photosynthetic apparatus activity (PI, PSII performance index) compared with the control (HPS). More light under DGlass conditions with and without LEDs (HPS+DGlass and LED+LED+DGlass) increased the tomato leaf thickness and number of mesophyll layers. The highest tomato fruit yield was obtained in the LED+LED+DGlass combination compared with the conventional growing technology (HPS). Fruits from this combination were characterised by the highest total soluble solids (TSS), carotenoids, and ascorbic acid content and the most favourable sugar/acid ratio. In the sensory evaluation, these fruits were sweeter and less acidic than fruits from the other crop combinations. The results obtained confirm the significant influence of light quality on plant growth, development, and yield. Full article

Tomato (Solanum lycopersicum L.), the second most important vegetable crop globally, faces a significant threat from various viral diseases. A newly emerging disease, characterised by distinctive shoestring symptoms on leaves and the development of unripe, small, and hard fruit, poses a serious challenge to tomato cultivation in India. An initial survey in an experimental field revealed more than 50% of the plants displayed symptoms of the shoestring disease, resulting in substantial reductions in fruit yield and quality. Transmission electron microscopy (TEM) and molecular analyses identified an isolate of the tomato mottle mosaic virus (ToMMV) in the affected plants. When the partially purified virus was mechanically inoculated into tomato cv. Pusa Ruby plants, it reproduced the characteristic shoestring symptoms, confirming its causal relationship with the disease. Notably, the present shoestring isolate of ToMMV (ToMMV-Tss) was found to induce similar shoestring symptoms in most of the major commercial tomato varieties when inoculated under controlled experimental conditions in the glasshouse, indicating its aggressive nature. Host range studies demonstrated that the ToMMV-Tss can infect several solanaceous species, while cucurbitaceous hosts remained unaffected. Moreover, the virus was found to be seed-transmissible, with a small percentage of seedlings from infected plants displaying symptoms. These findings underscore the significant impact of ToMMV on tomato production in India and emphasise the need for reliable diagnostic tools and effective management strategies to curb the spread and mitigate the impact of this virus on commercial tomato cultivation. Full article

To overcome the scarcity of fresh water, researchers have turned to investigating different techniques that enable using saline water to irrigate crops, aiming to increase the efficiency of using available water resources. A glasshouse experiment was conducted to investigate the yield responses of grafted and non-grafted (self-rooted) tomato plants grown hydroponically under three levels of water salinity (2.5, 6.0, and 9.5 dS m⁻¹). Three tomato varieties (Ghandowra-F1, Forester-F1, and Feisty-Red) were grafted onto five rootstocks (Maxifort, Unifort, Dynafort, Vivifort, and Beaufort). The implemented treatments were studied in terms of tomato fruit yield and quality parameters. Although increasing the concentration of salts in the nutrient solution led to a decrease in fruit yield, the moderate salinity level (S-2: 6.0 dS m⁻¹) showed its superiority over both low salinity (S-1: 2.5 dS m⁻¹) and high salinity (S-3: 9.5 dS m⁻¹) in terms of tomato yield parameters. The studied rootstocks did not significantly improve the tomato fruit yield, but the interaction between the grafting combinations and salinity was significant for both production and quality. More specifically, tomato plants grafted onto the rootstocks “Vivifort and Beaufort” rendered the highest yield at a low salinity level (S-1: 2.5 dS m⁻¹) and a moderate salinity level (S-2: 6.0 dS m⁻¹), respectively, while at high salinity (S-3: 9.5 dS m⁻¹), grafting did not improve tomato productivity, irrespective of the rootstock. These results confirm that tomatoes can be successfully grown under hydroponic systems using salinity levels of up to 6.0 dS m⁻¹ without sacrificing fruit yield and quality. Among the studied tomato varieties, Feisty-Red was found to be appropriate for hydroponic production. The results also demonstrated that Vivifort and Beaufort rootstocks are suitable for grafting hydroponic tomatoes under low and moderate salinity levels, respectively. Full article

Commercial formulations of beneficial microbes have been used to enrich the rhizosphere microbiome of tomato plants grown in pots located in a glasshouse. These plants have been subjected to attacks by soil-borne parasites, such as root-knot nematodes (RKNs), and herbivores, such as the miner insect Tuta absoluta. The development of both parasites and the symptoms of their parasitism were restricted in these plants with respect to plants left untreated. A mixture, named in the text as Myco, containing plant growth-promoting rhizobacteria (PGPR), opportunistic biocontrol fungi (BCF), and arbuscular mycorrhizal fungi (AMF) was more effective in limiting pest damage than a formulation containing the sole AMF (Ozor). Therefore, Myco-treated plants inoculated with RKNs were taken as a model for further studies. The PGPR contained in Myco were not able to reduce nematode infection; rather, they worsened symptoms in plants compared with those observed in untreated plants. Therefore, it was argued that both BCF and AMF were the microorganisms that colonized roots and stimulated the plant immune system against RKNs. Beneficial fungi colonized the roots by lowering the activities of the defense supporting enzymes endochitinases and β-1,3-glucanase. However, as early as three days after nematode inoculation, these enzyme activities and the expression of the encoding pathogenesis-related genes (PR-2, PR-3) were found to be enhanced in roots with respect to non-inoculated plants, thus indicating that plants had been primed against RKNs. The addition of paclobutrazol, which reduces salicylic acid (SA) levels in cells, and diphenyliodonium chloride, which inhibits superoxide generation, completely abolished the repressive effect of Myco on nematode infection. Inhibitors of copper enzymes and the alternative cyanide-resistant respiration did not significantly alter resistance induction by Myco. When Myco-treated plants were subjected to moderate water stress and inoculated with nematodes, they retained numbers of developed individuals in the roots similar to those present in regularly watered plants, in contrast to what occurred in roots of untreated stressed plants that hosted very few individuals because of poor nutrient availability. Full article

Tomato brown rugose fruit virus (ToBRFV) is a contact-transmitted tobamovirus affecting many tomato growing regions of the world. This study investigated the effects of different glasshouse surfaces on the survival of the virus; the efficacy of different disinfectants; and heat treatment against ToBRFV (surfaces included steel, aluminium, hard plastic, polythene, glass and concrete). A bioassay followed by ELISA was used to check virus viability. ToBRFV survived for at least 7 days on all surfaces tested and on some for at least 6 months. The virus survived for over two hours on hands and gloves. Hand washing was shown to be unreliable for the removal of the virus. Glutaraldehyde and quaternary ammonium compound disinfectants were effective at one hour on all surfaces. Some other disinfectants were effective at one hour of contact time, on all surfaces except concrete. Sodium hypochlorite was partially effective against ToBRFV, even on concrete. A 5 min soak of plastic trays in water at 90 °C was effective at denaturing ToBRFV; however, 5 min at 70 °C was not. Heating infected sap showed the thermal inactivation point to be 90 °C, confirming the hot water treatment results and showing that deactivation was due to the heat treatment and not a washing effect of the water. Full article

Bacterial wilt disease of tomato (Solanum lycopersicum L.), incited by Ralstonia solanacearum (Smith), is a serious agricultural problem in India. In this investigation, chemical mutagenic agents (NTG and HNO₂ treatment) and ultraviolet (UV) irradiation have been used to enhance the antagonistic property of Bacillus amyloliquefaciens DSBA-11 against R. solanacearum UTT-25 towards an effective management of tomato wilt disease. The investigation established the fact that maximum inhibition to R. solanacearum UTT-25 was exerted by the derivative strain MHNO₂-20 treated with nitrous acid (HNO₂) and then by the derivative strain MNTG-21 treated with NTG. The exertion was significantly higher than that of the parent B. amyloliquefaciens DSBA-11. These two potential derivatives viz. MNTG-21, MHNO₂-20 along with MUV-19, and a wild derivative strain of B. amyloliquefaciens i.e.,DSBA-11 were selected for GC/MS analysis. Through this analysis 18 major compounds were detected. Among the compounds thus detected, the compound 3-isobutyl hexahydropyrrolo (1,2), pyrazine-1,4-dione (4.67%) was at maximum proportion in the variant MHNO₂-20 at higher retention time (RT) of 43.19 s. Bio-efficacy assessment observed a record of minimum intensity (9.28%) in wilt disease and the highest bio-control (88.75%) in derivative strain MHNO₂-20-treated plants after 30 days of inoculation. The derivative strain MHNO₂-20, developed by treating B. amyloliquefaciens with nitrous acid (HNO₂), was therefore found to have a higher bio-efficacy to control bacterial wilt disease of tomato under glasshouse conditions than a wild-type strain. Full article

Tomato (Solanum lycopersicum) plants from a commercial glasshouse were identified with symptoms compatible with a tomato brown rugose fruit virus (ToBRFV) infection. Reverse transcription-PCR and quantitative PCR confirmed the presence of ToBRFV. Subsequently, the same RNA sample and a second from tomato plants infected with a similar tobamovirus, tomato mottle mosaic virus (ToMMV), were extracted and processed for high-throughput sequencing with the Oxford Nanopore Technology (ONT). For the targeted detection of ToBRFV, the two libraries were synthesized by using six ToBRFV sequence-specific primers in the reverse transcription step. This innovative target enrichment technology enabled deep coverage sequencing of ToBRFV, with 30% of the total reads mapping to the target virus genome and 57% mapping to the host genome. The same set of primers applied to the ToMMV library generated 5% of the total reads mapping to the latter virus, indicating that sequencing of similar, non-target viral sequences was also allowed. Further, the complete genome of pepino mosaic virus (PepMV) was also sequenced from the ToBRFV library, thus suggesting that, even using multiple sequence-specific primers, a low rate of off-target sequencing can usefully provide additional information on unexpected viral species coinfecting the same samples in an individual assay. These results demonstrate that targeted nanopore sequencing can specifically identify viral agents and has sufficient sensitivity towards non-target organisms to provide evidence of mixed virus infections. Full article

Appropriate foliar application of zinc (Zn) and zinc oxide nanoparticles (ZnO-NPs) is important for the proper growth and yield of tomato. However, the effects of foliar application of Zn and ZnO-NPs were not well-studied on tomato production. A pot experiment was conducted at glasshouse (8D) conditions under the Faculty of Agriculture, Universiti Putra Malaysia (UPM) to evaluate the effectiveness of Zn and ZnO-NPs on growth, yield, nutrient uptake, and fruit quality of tomatoes and to compare between the Zn nutrient and ZnO-NPs. Treatment combinations were 14 viz. T₁ = 0 (control), T₂ = 1500 ppm (mg/L) Zn nutrient, T₃ = 2000 ppm (mg/L) Zn nutrient, T₄ = 2500 ppm (mg/L) Zn nutrient, T₅ = 75 ppm ZnO nanoparticle, T₆ = 100 ppm ZnO nanoparticle, and T₇ = 125 ppm ZnO nanoparticle along with two tomato varieties. The experimental design was a split plot with four replications. Results indicated that foliar application of 100 ppm ZnO-NPs performed best in terms of growth parameters, physiological traits, yield attributes, yield, and quality traits of tomatoes. The same treatment (100 ppm ZnO-NPs) contributed to attain the highest nutrient uptake. Recovery use efficiency of Zn was highest with foliar application of 75 ppm ZnO-NPs. The highest yield increment (200%) over control was from foliar sprayed with 100 ppm ZnO-NPs. Comparing the two varieties, MARDI Tomato-3 (MT3) showed better than MARDI Tomato-1 (MT1). As is appears from the results, foliar application of zinc oxide nanoparticles was more efficient than conventional zinc fertilizer. Therefore, the foliar sprayed with 100 ppm ZnO-NPs can be suggested to improve quantity and quality of tomato in glasshouse soil conditions. Full article

Bacillus spp. has the potential to control bacterial and fungal diseases of crops. In vitro study, Bacillus amyloliquefaciens DSBA-11 showed best to inhibit the growth of Ralstonia pseudosolanacearum as compared to Bacillus cereus JHTBS-7, B. pumilus MTCC-7092, B. subtilis DTBS-5 and B. licheniformis DTBL-6.Three primers sets from nucleotide sequences of polyketide antibiotic synthase genes viz., macrolactin, difficidin and bacillaene of B. amyloliquefaciens FZB42 were designed and standardized protocol for simultaneous detection of polyketide antibiotics-producing strains of Bacillus spp. by multiplex—PCR with products size of 792 bp, 705 bp and 616 bp respectively. All the strains of B. amyloliquefaciens contained three polyketide antibiotic synthase genes, and B. subtilis possessed difficidin and macrolactin, whereas B. cereus JHTBS-7, B. pumilus MTCC-7092 and B. licheniformis DTBL-6 did not contain any polyketide antibiotic genes. By using this technique, polyketide-producing strains of Bacillus spp. were screened within a short period with high accuracy. These polyketide synthase genes were cloned by using a T&A vector to study the role of these genes in producing antibiotics that suppressed the growth of R. pseudosolanacearum under both in vitro and in vivo conditions. Bio-efficacy of cloned products of these genes macrolactin, bacillaene, and difficidin along with parent strain B. amyloliquefaciens DSBA-11 inhibited the growth of R. pseudosolanacearum and formed 1.9 cm², 1.9 cm², 1.7 cm² and 3.3 cm² inhibition area under in vitro conditions respectively. Minimum bacterial wilt disease intensity (29.3%) with the highest biocontrol efficacy (57.72%) was found in tomato cv. Pusa Ruby (susceptible to wilt disease) was treated with B. amyloliquefaciens DSBA-11 followed by cloned products of difficidin and macrolactin under glasshouse conditions. Hence, the developed multiplex protocol might be helpful for screening polyketide antibiotics producing potential strains of Bacillus spp. from soil which can apply for managing the wilt disease of tomatoes. The polyketide antibiotics produced by bacteria might have a significant role suppression of R. pseudosolanacearum due to the disintegration of cells. Full article

The bacterial canker disease of tomato caused by Clavibacter michiganensis subsp. michiganensis (Cmm) has been reported to adversely affect the tomato cultivation in the NE hilly regions of India. Defense inducers such as salicylic acid (SA), isonicotinic acid (INA), benzothiadiazole (BTH) and lysozyme were used as prophylactic and curative sprays at different concentrations to test their efficacy in inducing resistance in tomato plants against Cmm under protected conditions. The induced resistance was studied through the alteration in the activities of oxidative stress marker enzymes (PAL, PO, PPO, TPC and PR-2 protein), hydrogen peroxide formation in leaf tissues and lignin accumulation in stem tissues, as well as through the reduction in disease severity under glasshouse conditions. The results of the present study revealed that the enzymatic activity, hydrogen peroxide formation and lignin production were significantly higher in the BTH (500 ppm)-treated leaves than in those observed in the control. The lowest disease incidence was recorded when BTH was applied as a prophylactic spray (27.88%) in comparison to being applied as a curative spray (53.62%), thereby suggesting that a defense inducer, BTH, shows antibacterial activity against Cmm, reduces disease incidence severity and induces defense responses in the tomato plant. Full article

In order to investigate the effects of water-fertilizer-air-coupling drip irrigation on soil health status, including soil aeration (SA), enzyme activity (EA) and microbial biomass (MB), and its response relationship, this glasshouse experiment was conducted using tomato as the test crop, and we designed two fertilization gradients of 135 and 180 kg N·ha⁻¹, two irrigation levels of 0.6-fold and 1.0-fold of the crop-pan coefficient, and two aeration treatments of 5 and 15 mg·L⁻¹ for the three-factor and two-level completely randomized block experiment. The effects of soil dissolved-oxygen concentration, oxygen diffusion rate, soil respiration rate, soil urease, catalase, phosphatase activities and soil microbial biomass were systematically monitored and analyzed in the middle and at the end of crop growth. A structural equation model was used to comprehensively analyze the response relationship among relevant influencing factors. The results showed that coupled drip irrigation increased the soil’s dissolved oxygen, oxygen diffusion rate and soil respiration rate by 14.05%, 30.14% and 53.74%, respectively. Soil urease, catalase and phosphatase activities increased by 22.83%, 93.01% and 61.35%, respectively. The biomass of bacteria, fungi and actinomycetes increased by 49.06%, 50.18% and 20.39%, respectively. The results of a structural equation model analysis showed that water-fertilizer-air-coupling drip irrigation could effectively improve soil health status, and the descending order of influence was MB > EA > SA. This study provides scientific knowledge to reveal the improvement of soil health status by water-fertilizer-air-coupling drip irrigation. Full article

Reducing food loss and waste (FLW) is one strategy to limit the environmental impact of the food supply chain. Australian data suggest that primary production accounts for 31% of national FLW, but there are no comparable data in New Zealand. This study aimed to measure food loss and explore food loss drivers for one of New Zealand’s largest tomato growers by weighing and visually assessing tomato losses at the glasshouse, packhouse and sales warehouse. Qualitative interviews were also held with the grower (n = 3), employees (n = 10), and key industry stakeholders (n = 8). Total food loss for this greenhouse tomato grower was 16.9% of marketed yield, consisting of 13.9% unharvested tomatoes, 2.8% rejected at the glasshouse and 0.3% rejected at the packhouse. The grower’s tomato loss predominantly resulted from commercial factors such as market price, competitor activity and supply and demand. Similar issues were recognized throughout the New Zealand horticulture sector. Commercial factors, in particular, are challenging to address, and collaboration throughout the supply chain will be required to help growers reduce food losses. Full article

Countries located in the Mediterranean region share many common features in terms of agricultural sustainability and economic realities of modern farming, as they are affected by water scarcity, energy use and climate suitability. Greenhouses are considered as a mitigation measure to combat climate change and as a sustainable production system. The majority of greenhouses in the Mediterranean region are rudimentary, while those in Central and North Europe are characterized by equipment of a high technological level for greenhouse climate and fertigation management. However, the technological innovations and research originating from Central and North Europe glasshouses may not be appropriate for use in Mediterranean plastic greenhouses when considering the trade-off between agronomic needs and potential energy savings. Identifying energy measures suitable for the local climate will improve energy efficiency and crop performance toward the goal of greenhouse sustainability. This review mainly focuses on renewable and energy-efficient control systems in Mediterranean greenhouses, where crops such as tomato and cucumber are widely grown. Full article

Currently, a great portion of tomatoes is produced by soilless cultivation systems and the substrate selection among the various materials is one of the most important factors affecting yield and quality traits. On the other hand, grafting has been successfully used in soilless systems to ensure long-term cultivation. However, due to the high cost of grafted seedlings, plant training systems are sought. Given the fact that most literature refers to studies intended to mainly reveal production differences among treatments and the quality aspect was secondary, the present study was focused on the evaluation of tomato fruit functionality, flavor and visual traits. Tomato plants cv ‘Beef Bang F1’ were cultivated in a glasshouse hydroponic culture in four substrates: rockwool slabs, perlite in sacks, pumice in sacks and pumice in 9 L pots. The type of cultivated plants used were self-rooted or grafted onto ‘Defensor’ trained in single and double stems. Tomato fruit were harvested three times during the season (6 June, 31 July, 6 November). The fruit quality was measured based on visual (average fruit mass, and Minolta color values), flavor (dry mass, soluble solids content, titratable acidity, pH, flesh firmness) as well as functional traits (total phenolic content, ascorbic acid, lycopene, β-carotene, total carotenoid content and antioxidant capacity). Harvest time was the most important factor followed in many of these cases by the substrate (flavor and functional traits), as well as in certain cases by the plant grafting/training (flavor traits and antioxidants) or by both in some flavor traits and antioxidants. Correlation of color values with lycopene, though significant, was weak. Each individual harvest time revealed the rise in different parameters. Pumice, whether used in pot or in sack, enhanced the visual and flavor attributes the most, self-rooted plants and mid-summer harvest resulted in the highest tomato fruit quality. Full article

Urban agriculture requires sustainable solutions to secure its water resources. A wicking bed (WB) is a simple system that could provide high yield and water use efficiency. This single trial glasshouse study compares the performance of a WB and surface irrigation (SI) system for growing tomatoes (Solanum lycopersicum L.) using fresh (FW) and recycled water (RW). The performance of both treatments was compared when irrigating 2 days per week and for two environmental conditions (indoor and outdoor). In addition, the reliability of using FW and RW at a 7-day irrigation interval was studied for WBs alone. Results showed that the irrigation water use efficiency (kg/m³) and the yield (kg/plant) are significantly different only between WB (FW) and SI (RW) considering all conditions. The accumulation of salts and the sodium absorption ratio (SAR) were high in the surface layer of WBs compared to SI. This indicates that the use of RW affects the level of salinity and sodicity in soil, which in turn may decrease the yield. However, WBs perform similar to, if not better than, SI with FW. The WBs show the advantage of reducing the leachate of nutrients into groundwater, compared to SI systems. Further research into irrigation and nutrient management in WBs to reduce the effect of salinity at the surface is recommended to increase the efficiency of the system. Full article