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Keywords = dental unit chair

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32 pages, 5836 KiB  
Article
Āsana for Back, Hips and Legs to Prevent Musculoskeletal Disorders among Dental Professionals: In-Office Yóga Protocol
by Maria Giovanna Gandolfi, Fausto Zamparini, Andrea Spinelli and Carlo Prati
J. Funct. Morphol. Kinesiol. 2024, 9(1), 6; https://doi.org/10.3390/jfmk9010006 - 22 Dec 2023
Cited by 7 | Viewed by 6704
Abstract
Dental professionals are exposed to significant unavoidable physical stress, and theoretical ergonomic recommendations for a sitting workplace are inapplicable in many dental activities. Work-related musculoskeletal disorders (WMSDs) represent a serious health problem among dental professionals (prevalence: 64–93%), showing involvement of 34–60% for the [...] Read more.
Dental professionals are exposed to significant unavoidable physical stress, and theoretical ergonomic recommendations for a sitting workplace are inapplicable in many dental activities. Work-related musculoskeletal disorders (WMSDs) represent a serious health problem among dental professionals (prevalence: 64–93%), showing involvement of 34–60% for the low back and 15–25% for the hips. Muscle stress; prolonged sitting; forward bending and twisting of the torso and head; unbalanced working postures with asymmetrical weight on the hips and uneven shoulders; and others are inevitable for dental professionals. Therefore, the approach for the prevention and treatment of WMSDs must be therapeutic and compensatory. This project was conceived to provide a Yoga protocol for dental professionals to prevent or treat WMSDs from a preventive medicine perspective, and it would represent a Yoga-based guideline for the self-cure and prevention of musculoskeletal problems. Methods: Specific Yoga positions (āsana, such as Virāsana, Virabhadrāsana, Garudāsana, Utkatāsana, Trikonāsana, Anuvittāsana, Chakrāsana, Uttanāsana, Pashimottanāsana) have been selected, elaborated on and adapted to be practiced in a dental office using a dental stool or the dental office walls or a dental unit chair. The protocol is specifically devised for dental professionals (dentists, dental hygienists and dental assistants) and targeted for the low back, hips and legs (including knees and ankles). The protocol includes Visranta Karaka Sthiti (supported positions) in sitting (Upavistha Sthiti) and standing (Utthistha Sthiti) positions, twisting/torsions (Parivrtta), flexions/forward bend positions (Pashima) and extensions/arching (Purva) for musculo-articular system decompression and mobilization. Results: Over 60 Yogāsana—specifically ideated for back detensioning and mobilization, lumbar lordosis restoration, trunk side elongation, hip release and leg stretches and decontraction—are shown and described. The paper provides a meticulous description for each position, including the detailed movement, recommendations and mistakes to avoid, and the breathing pattern (breath control) in all the breath-driven movements (āsana in vinyāsa). An exhaustive analysis of posture-related disorders affecting the lower body among dental professionals is reported, including low-back pain, hip pain and disorders, piriformis syndrome and quadratus femoris dysfunction (gluteal pain), iliopsoas syndrome, multifidus disorders, femoroacetabular and ischiofemoral impingement, spinopelvic mobility, lumbopelvic rhythm, impairment syndromes, lower crossed syndrome, leg pain, knee pain and ankle disorders. Conclusions: A detailed guideline of āsana for low-back decompression, hip joint destress, piriformis and gluteal muscle release, lumbar lordosis recovery and a spinopelvic mobility increase has been elaborated on. The designed Yogāsana protocol represents a powerful tool for dental professionals to provide relief to retracted stiff muscles and unbalanced musculoskeletal structures in the lower body. Full article
(This article belongs to the Special Issue Advances in Musculoskeletal Physiotherapy)
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13 pages, 3110 KiB  
Article
Impact of Chlorine Dioxide on Pathogenic Waterborne Microorganisms Occurring in Dental Chair Units
by Theresa Isabella Maria Krüger, Susann Herzog, Alexander Mellmann and Thorsten Kuczius
Microorganisms 2023, 11(5), 1123; https://doi.org/10.3390/microorganisms11051123 - 26 Apr 2023
Cited by 7 | Viewed by 2448
Abstract
Bacterial contamination is a problem in dental unit water lines with the consequence of implementing regular disinfection. In this study, the short-term impact of chlorine dioxide (ClO2) treatment was investigated on the microorganisms Legionella pneumophila and L. anisa, Pseudomonas aeruginosa [...] Read more.
Bacterial contamination is a problem in dental unit water lines with the consequence of implementing regular disinfection. In this study, the short-term impact of chlorine dioxide (ClO2) treatment was investigated on the microorganisms Legionella pneumophila and L. anisa, Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus. The environmental background was proven as an important factor regarding the tolerance to 0.4 mg/L ClO2 as saline and phosphate-buffered saline resulted in a higher bacterial reduction than tap water. Gram-positive microorganisms demonstrated higher robustness to ClO2 than Gram-negative, and microorganisms adapted to tap water showed increased stability compared to cultured cells. At high densities, substantial numbers of bacteria were able to withstand disinfection, whereby the use of 4.6 mg/L ClO2 increased the inactivation rate. A massive cell decrease occurred within the first 5 minutes with subsequent plateau formation or slowed cell reduction upon further exposure. This biphasic kinetics cannot be explained by a ClO2 depletion effect alone, because the probability of bacterial subpopulations with increased tolerance should be taken into account, too. Our results prove high disinfection efficiency to microorganisms that were rather found in correlation to the level of bacterial contamination and background solutions than the chosen concentration for ClO2 treatment itself. Full article
(This article belongs to the Section Public Health Microbiology)
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11 pages, 279 KiB  
Article
Evaluation of the Effectiveness of Overheated Dry-Saturated Steam Disinfection in the Control of the Dental Chair Contamination by Bioluminescence Analysis: A Pilot In Vitro Study
by Valentina Luppieri, Manuela Giangreco, Maddalena Chermetz, Luca Ronfani and Milena Cadenaro
Appl. Sci. 2023, 13(6), 3685; https://doi.org/10.3390/app13063685 - 14 Mar 2023
Viewed by 1485
Abstract
This study aimed to evaluate, through Adenosine triphosphate (ATP) bioluminescence analysis, the effectiveness of an overheated dry-saturated steam device (Polti Sani System) in decreasing the superficial microbial contamination on dental chairs’ surfaces after 30 s steam disinfection (T1) in comparison to [...] Read more.
This study aimed to evaluate, through Adenosine triphosphate (ATP) bioluminescence analysis, the effectiveness of an overheated dry-saturated steam device (Polti Sani System) in decreasing the superficial microbial contamination on dental chairs’ surfaces after 30 s steam disinfection (T1) in comparison to baseline (T0), i.e., at the end of an aerosol-generating procedure (AGDP), and to investigate any differences in the tested surfaces’ contamination at T0 in relation to the surface’s type. Three dental chair surfaces (scialytic lamp, control button panel, spit bowl), sized 10 × 10 cm each, were swabbed and analyzed before and after steam application. The procedure was repeated 20 times for a total of 60 before–after evaluations. Non-parametric tests were used to analyze Relative Light Unit (RLU) values and categorical data on the ATP molecules’ amount detected on the tested surfaces. Statistically significant differences were found for both RLU and categorical data for all surfaces, and each type of surface evaluated at T0 and T1 (p < 0.05). Differences in RLU among the tested surfaces at T0 were not significant. By reducing the microbial contamination on the evaluated surfaces, the overheated dry-saturated steam system was an effective measure for the disinfection of the dental chair’s surfaces after AGDPs, potentially reducing the risk of cross-infections. Full article
(This article belongs to the Special Issue Clinical Applications for Dentistry and Oral Health, 2nd Volume)
26 pages, 4132 KiB  
Article
Āsana for Neck, Shoulders, and Wrists to Prevent Musculoskeletal Disorders among Dental Professionals: In-Office Yóga Protocol
by Maria Giovanna Gandolfi, Fausto Zamparini, Andrea Spinelli and Carlo Prati
J. Funct. Morphol. Kinesiol. 2023, 8(1), 26; https://doi.org/10.3390/jfmk8010026 - 20 Feb 2023
Cited by 15 | Viewed by 8535
Abstract
Extensive literature reports an increase in physical disorders (pain, pathologies, dysfunctions) and mental malaise/uneasiness (stress, burnout) affecting dental professionals in relation to fast and pressing rhythms of work, long working hours, increasingly demanding patients, ever-evolving technologies, etc. This project has been conceived to [...] Read more.
Extensive literature reports an increase in physical disorders (pain, pathologies, dysfunctions) and mental malaise/uneasiness (stress, burnout) affecting dental professionals in relation to fast and pressing rhythms of work, long working hours, increasingly demanding patients, ever-evolving technologies, etc. This project has been conceived to bring the science of yoga around the world to dental professionals as a preventive (occupational) medicine and to provide knowledge and means for self-care. Yoga is a concentrative self-discipline of the mind, senses, and physical body, that requires regular daily exercise (or meditation), attention, intention, and disciplined action. M&M: The study aimed to design a Yoga protocol specifically devised for dental professionals (dentists, dental hygienists, and dental assistants) including positions (āsana) to be practiced/used in the dental office. The protocol is targeted for the upper body, namely neck, upper back, chest, shoulder girdle, and wrists, being areas greatly affected by work-related musculoskeletal disorders. This paper represents a yoga-based guideline for the self-cure of musculoskeletal disorders among dental professionals. Results: The protocol includes both sitting (Upavistha position) and standing (Utthana or Sama position) āsana, with twisting (Parivrtta), side bending (Parsva), flexion and forward bending (Pashima), and extension and arching (Purva) āsana to mobilize and decompress, and to provide nourishment and oxygen to the musculo-articular system. The paper delivers different concepts and theories developed and deepened by the authors and introduces and spreads yoga as a medical science among dental professionals for the prevention and treatment of work-related musculoskeletal disorders. We articulate notions ranging from stretching out using the vinyāsa method (breath-driven movement) and inward-focused attention to contemplative/concentrative science, interoceptive attention, self-awareness, the mind–body connection, and receptive attitude. The theory of “muscles are bone ties” is coined and delivered with regard to tensegrity musculoskeletal fascial structures connecting, pulling together, and nearing the bone segments where they are anchored. The paper describes over 60 āsana envisaged to be performed on dental stools or using the walls of a dental office or a dental unit chair. A detailed guideline on the work-related disorders that can find relief with the protocol is provided, including the description of breath control for the practice of āsana in vinyāsa. The foundations of the technique reside in the Iyengar Yoga method and Parināma Yoga method. Conclusions: This paper represents a guideline for self-cure in the prevention or treatment of musculoskeletal disorders affecting dental professionals. Yoga is a powerful concentrative self-discipline able to provide physical and mental well-being, representing great help and support in daily life and business for dental professionals. Yógāsana restores retracted and stiff muscles, giving relief to the strained and tired limbs of dental professionals. Yoga is not intended for flexible or physically performing persons but for people who decide to take care of themselves. The practice of specific āsana represents a powerful tool for the prevention or treatment of MSDs related to poor posture, forward head, chronic neck tension (and related headache), depressed chest, compressive disorders on wrists and shoulders as carpal tunnel, impingement syndromes, outlet syndrome, subacromial pain syndrome and spinal disc pathologies. Yoga, as an integrative science in medicine and public health, represents a powerful tool for the prevention and treatment of occupational musculoskeletal disorders and an extraordinary path for the self-care of dental professionals, sitting job workers, and healthcare providers suffering from occupational biomechanical stresses and awkward postures. Full article
(This article belongs to the Topic Role of Exercise in Musculoskeletal Disorders)
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12 pages, 3299 KiB  
Article
A Study on the Psychological Noise Reduction Effect on Dental Handpiece Noise through the Bone Conduction Speaker Equipped Unit Chair and Notch-Filtered Music
by Yeabon Jo, Woojin Kang, Sungwoo Hong, Joseph Vermont Bandoy, Hyuk-Sang Kwon, Heejung Kim and Eunsung Song
Appl. Sci. 2023, 13(1), 359; https://doi.org/10.3390/app13010359 - 27 Dec 2022
Cited by 1 | Viewed by 3377
Abstract
Anxiety in dental patients has caused inconvenient experiences during their dental visits due to the noise generated by the dental handpiece. High-frequency sounds generated by the handpiece have been challenging to reduce using the active control method that targets low-frequency sounds, as well [...] Read more.
Anxiety in dental patients has caused inconvenient experiences during their dental visits due to the noise generated by the dental handpiece. High-frequency sounds generated by the handpiece have been challenging to reduce using the active control method that targets low-frequency sounds, as well as the difficulty in applying the noise control method using sound-absorbing materials, because the size of the handpiece is small. As an alternative, a method that can reduce noise and provide stability by playing music to patients is being studied. However, in most studies, there are inconveniences such as the need to turn the music volume higher to cover dental handpiece noise or having to wear headphones to play music. In this study, in order to reduce this inconvenience and optimize the noise reduction effect of music, we propose a technology that converts music into sound masking and a unit chair equipped with a bone conduction speaker that plays music, and through clinical trials with 35 patients, it was confirmed that the proposed system made the patients emotionally stable. In addition, by analyzing the causes of these emotional changes, it suggests that the preferred genre of music by patients should also be considered. Full article
(This article belongs to the Special Issue Soundscapes and Ambient Noise)
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12 pages, 334 KiB  
Article
Work-Related Musculoskeletal Disorders among Dentists in the United Arab Emirates: A Cross-Sectional Study
by Amal Hussein, Mahmoud Mando and Ricardas Radisauskas
Medicina 2022, 58(12), 1744; https://doi.org/10.3390/medicina58121744 - 28 Nov 2022
Cited by 10 | Viewed by 4378
Abstract
Background and Objectives: A dental environment possesses a serious ergonomic health issue on the dental team members which in turn contributes to the development of work-related musculoskeletal disorders (WMSDs). The present research is aimed at evaluating the frequency of musculoskeletal disorders and their [...] Read more.
Background and Objectives: A dental environment possesses a serious ergonomic health issue on the dental team members which in turn contributes to the development of work-related musculoskeletal disorders (WMSDs). The present research is aimed at evaluating the frequency of musculoskeletal disorders and their associated sociodemographic and work environment risk factors among dentists in the United Arab Emirates. Material and Methods: In this cross-sectional study, a pretested and validated questionnaire was sent via email as well as on different social media platforms to a total of 497 dentists. A total of 179 dentists completed the study survey, with a total response rate of 36%. A binary logistic regression model was conducted to identify significant risk factors associated with WMSDs. Results: Dentists in the United Arab Emirates experience a high prevalence of work-related musculoskeletal disorders (90.4%) which were associated with high levels of stress in the work environment. Furthermore, dentists in the private sector worked more clinical hours per day (p = 0.000) and had more financial stress (p = 0.007) as compared to those in the governmental sector. Gender (p = 0.007), age (p = 0.01), monthly income (p = 0.073), work experience (p = 0.037), number of patients treated per day (p = 0.049), and the use of an ergonomic dental chair (p = 0.005) were all factors associated with a greater number of affected body regions by WMSDs. Multivariate binary logistic regression for the number of regions affected by WMSDs revealed that not using an ergonomic dental chair (OR 2.70, 95% CI, 1.14–6.36) and high stress in the work environment (OR 1.31, 95% CI 1.02 to1.67) were associated with more body regions being affected by WMSDs. Conclusions: This study highlights the high prevalence rate of WMSDs among dentists in the UAE. Future research should be directed towards reducing stress in the work environment, increasing awareness regarding the importance of an ergonomic dental chair, and reducing gaps between private and governmental practices. Full article
(This article belongs to the Section Epidemiology & Public Health)
12 pages, 1809 KiB  
Article
How Does the Use of an Intraoral Scanner Affect Muscle Fatigue? A Preliminary In Vivo Study
by KeunBaDa Son, Ji-Min Lee, Young-Tak Son, Jin-Wook Kim, Myoung-Uk Jin and Kyu-Bok Lee
Bioengineering 2022, 9(8), 358; https://doi.org/10.3390/bioengineering9080358 - 1 Aug 2022
Cited by 8 | Viewed by 2497
Abstract
The purpose of this study was to evaluate muscle activation and fatigue in the operator during tooth preparation and intraoral scanning by simulating these tasks in two types of dental unit chair systems (UCS). Six participants were recruited, and the above tasks were [...] Read more.
The purpose of this study was to evaluate muscle activation and fatigue in the operator during tooth preparation and intraoral scanning by simulating these tasks in two types of dental unit chair systems (UCS). Six participants were recruited, and the above tasks were simulated. Electrodes were placed on the skin over five types of muscles (arm, neck, and shoulder muscles), and the maximal voluntary contraction (MVC) was measured. Electromyography (EMG) was assessed during the simulation, and EMG values were normalized using MVC. The root mean square (RMS) EMG (%MVC) and muscle fatigue (%) were calculated. Owing to a lack of normal distribution of the data, Mann–Whitney U test and Kruskal–Wallis H test were performed for statistical comparison, and Bonferroni adjustment was performed for multiple comparisons (α = 0.05). There was no significant difference in RMS EMG between the two types of dental UCS (intraoral scanning, p = 0.237; tooth preparation, p = 0.543). Moreover, the RMS EMG and muscle fatigue were not significantly different between the two tasks (p > 0.05). There was significant muscle fatigue after the intraoral scanner use was simulated thrice (p < 0.001). It is necessary to refrain from performing continuous intraoral scanning and tooth preparation and to take appropriate rest to reduce the incidence of musculoskeletal disorders in dentists in clinical settings. Full article
(This article belongs to the Special Issue Biomechanics-Based Motion Analysis)
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14 pages, 533 KiB  
Review
Various Coated Barrier Membranes for Better Guided Bone Regeneration: A Review
by Ji-Youn Kim and Jun-Beom Park
Coatings 2022, 12(8), 1059; https://doi.org/10.3390/coatings12081059 - 26 Jul 2022
Cited by 7 | Viewed by 3411
Abstract
A good barrier membrane is one of the important factors for effective guided bone/tissue regeneration (GBR/GTR) in the case of periodontal bone defects. Several methods are being discussed to overcome and improve the shortcomings of commercially available membranes. One of the methods is [...] Read more.
A good barrier membrane is one of the important factors for effective guided bone/tissue regeneration (GBR/GTR) in the case of periodontal bone defects. Several methods are being discussed to overcome and improve the shortcomings of commercially available membranes. One of the methods is to coat the membrane with bioactive materials. In this study, 41 studies related to coated membranes for GBR/GTR published in the last 5 years were reviewed. These studies reported coating the membrane with various bioactive materials through different techniques to improve osteogenesis, antimicrobial properties, and physical/mechanical properties. The reported studies have been classified and discussed based on the purpose of coating. The goal of the most actively studied research on coating or surface modification of membranes is to improve new bone formation. For this purpose, calcium phosphate, bioactive glass, polydopamine, osteoinduced drugs, chitosan, platelet-rich fibrin, enamel matrix derivatives, amelotin, hyaluronic acid, tantalum, and copper were used as membrane coating materials. The paradigm of barrier membranes is changing from only inert (or biocompatible) physical barriers to bioactive osteo-immunomodulatory for effective guided bone and tissue regeneration. However, there is a limitation that there exists only a few clinical studies on humans to date. Efforts are needed to implement the use of coated membranes from the laboratory bench to the dental chair unit. Further clinical studies are needed in the patients’ group for long-term follow-up to confirm the effect of various coating materials. Full article
(This article belongs to the Special Issue Recent Advances in Coatings of Implant and Dental Biomaterials)
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14 pages, 583 KiB  
Article
Satisfaction Factors with a Dental Unit Chair System in South Korea: A Dentist’s Perspective
by Keunbada Son, Young-Tak Son, Myoung-Uk Jin and Kyu-Bok Lee
Healthcare 2022, 10(3), 437; https://doi.org/10.3390/healthcare10030437 - 25 Feb 2022
Cited by 1 | Viewed by 4742
Abstract
This study aimed to survey users’ satisfaction with a dental unit chair in order to highlight the elements affecting the dentist’s satisfaction. The questionnaire items were drawn up with seven components that constitute a dental unit chair, including the light, patient seat, foot [...] Read more.
This study aimed to survey users’ satisfaction with a dental unit chair in order to highlight the elements affecting the dentist’s satisfaction. The questionnaire items were drawn up with seven components that constitute a dental unit chair, including the light, patient seat, foot controller, water fountain and cuspidor, monitor, bracket table and controller, and dentist chair. With these questionnaire elements, a pilot experiment was conducted to test the reliability, and reliability analysis was conducted. The scale reliability was checked using Cronbach’s alpha coefficient. Bartlett’s test of sphericity, the Kaiser-Meyer-Olkin (KMO) measure, and factor analysis were performed to test whether the items would constitute appropriate questionnaire items for the survey. The survey was conducted with 26 dentists with more than three years of clinical experience. A correlation analysis was conducted using Pearson’s correlation coefficient (PCC) (α = 0.05) to analyze the impact of the factors on the overall satisfaction with the dental unit chair. The items that were strongly correlated with the overall satisfaction score of the dental unit chair were the design and appearance quality of the dental unit chair (PCC = 0.781), its maintenance (PCC = 0.784), and the overall satisfaction with the water fountain and cuspidor (PCC = 0.703) (p < 0.05). Most of the questionnaire items could affect the overall satisfaction with the dental unit chair. Additionally, because the design and appearance quality, maintenance, and overall satisfaction with the water fountain and cuspidor may have the greatest impact on the overall satisfaction with the dental unit chair, the improvement of these elements may bring about the enhancement of the overall satisfaction. Full article
(This article belongs to the Collection Dentistry, Oral Health and Maxillofacial Surgery)
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13 pages, 10046 KiB  
Article
Assessment of Color Perception and Preference with Eye-Tracking Analysis in a Dental Treatment Environment
by Eun-Sung Song, Won-Hyeon Kim, Beom-Hui Lee, Dong-Wook Han, Jong-Ho Lee and Bongju Kim
Int. J. Environ. Res. Public Health 2021, 18(15), 7981; https://doi.org/10.3390/ijerph18157981 - 28 Jul 2021
Cited by 10 | Viewed by 4337
Abstract
Nowadays, medical facilities are developing their treatment environment to provide better services to their patients. In particular, dental hospitals have been considered uncomfortable and uninviting spaces, which needs to change so that people can visit easily and feel more relaxed. However, only a [...] Read more.
Nowadays, medical facilities are developing their treatment environment to provide better services to their patients. In particular, dental hospitals have been considered uncomfortable and uninviting spaces, which needs to change so that people can visit easily and feel more relaxed. However, only a few systematic studies have reported on the demand for building a comfortable space. This study aimed to investigate gaze characteristics based on a color preference survey of the dental unit chair, which has the most influence on spatial perception in the dental treatment environment, using an eye tracking technique for color. The results of this study showed that the color perception by eye tracking and the color preference by survey did not tend to match. The color most viewed by a majority of subjects was pink, which attracted a high level of attention, regardless of personal preference. In addition, for the psychological color images associated with color preference, the subjects tended to prefer images such as warmth, friendliness, and calmness. This appeared to reflect the psychology of the subjects who wished to replace their feelings of anxiety or fear when going to the dental hospital with comfort and tranquility. Therefore, colors that can provide comfort and tranquility to patients should be considered first as visual elements (e.g., brown) in creating a dental treatment environment. Full article
(This article belongs to the Special Issue Advances in Pediatric Dental Care in Relation to Public Health)
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12 pages, 2186 KiB  
Article
Marine-Source Quorum Quenching Enzyme YtnP to Improve Hygiene Quality in Dental Units
by Xiaohui Sun, Philip Hill, Jia Liu, Jing Qian, Yuting Ma and Shufeng Zhou
Mar. Drugs 2021, 19(4), 225; https://doi.org/10.3390/md19040225 - 16 Apr 2021
Cited by 18 | Viewed by 3041
Abstract
Biofilm in dental unit water lines may pose a health risk to patients and dental practitioners. An AdiC-like quorum quenching enzyme, YtnP, was cloned from a deep-sea probiotic Bacillus velezensis, and heterologously expressed in E. coli to examine the application on the [...] Read more.
Biofilm in dental unit water lines may pose a health risk to patients and dental practitioners. An AdiC-like quorum quenching enzyme, YtnP, was cloned from a deep-sea probiotic Bacillus velezensis, and heterologously expressed in E. coli to examine the application on the improvement of hygiene problems caused by biofilm infection of Pseudomonas aeruginosa in dental units. Pseudomonas bacteria were isolated from dental chair units and used to grow static biofilms in the laboratory. A water filter system was designed to test the antifouling activity of YtnP in Laboratory, to simulate the biofilm contamination on water filter in dental unit water lines. The results demonstrated that the enzyme of YtnP was able to degrade the N-acyl homoserine lactones, significantly inhibited the EPS generation, biofilm formation, and virulence factors production (pyocyanin and rhamnolipid) of P. aeruginosa, and was efficient on the antifouling against P. aeruginosa. The findings in this study indicated the possibility of YtnP as novel disinfectant reagent for hygiene treatment in dental units. Full article
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12 pages, 1657 KiB  
Article
Environmental Detection of SARS-CoV-2 Virus RNA in Health Facilities in Brazil and a Systematic Review on Contamination Sources
by Vania Aparecida Vicente, Bruno Paulo Rodrigues Lustosa, Maria Eduarda Grisolia, Caroline Pavini Beato, Eduardo Balsanelli, Viviane de Souza Gubert Fruet, Meri Bordignon Nogueira, Sonia Maria Raboni, Katherine Athayde Teixeira Carvalho, Izadora Cervelin Flôr, Morgana Ferreira Voidaleski, Ramiro Gonçalves Etchepare, Jacques F. Meis, Vanete Thomaz Soccol and Emanuel Maltempi Souza
Int. J. Environ. Res. Public Health 2021, 18(7), 3824; https://doi.org/10.3390/ijerph18073824 - 6 Apr 2021
Cited by 12 | Viewed by 5042
Abstract
SARS-CoV-2 environmental monitoring can track the rate of viral contamination and can be used to establish preventive measures. This study aimed to detect by RT-PCR the presence of SARS-CoV-2 from inert surface samples in public health settings with a literature review about surface [...] Read more.
SARS-CoV-2 environmental monitoring can track the rate of viral contamination and can be used to establish preventive measures. This study aimed to detect by RT-PCR the presence of SARS-CoV-2 from inert surface samples in public health settings with a literature review about surface contamination and its burden on spread virus. Samples were collected from health settings in Curitiba, Brazil, between July and December 2020. A literature review was conducted using PRISMA. A total of 711 environmental surface samples were collected from outpatient areas, dental units, doctors’ offices, COVID-19 evaluation areas, and hospital units, of which 35 (4.9%) were positive for SARS-CoV-2 RNA. The frequency of environmental contamination was higher in primary care units than in hospital settings. The virus was detected on doctors’ personal items. Remarkably, the previously disinfected dental chair samples tested positive. These findings agree with those of other studies in which SARS-CoV-2 was found on inanimate surfaces. Detection of SARS-CoV-2 RNA on surfaces in public health settings, including those not meant to treat COVID-19, indicates widespread environmental contamination. Therefore, the intensification of disinfection measures for external hospital areas may be important for controlling community COVID-19 dissemination. Full article
(This article belongs to the Section Environmental Microbiology)
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12 pages, 2577 KiB  
Article
Risks of Aerosol Contamination in Dental Procedures during the Second Wave of COVID-19—Experience and Proposals of Innovative IPC in Dental Practice
by Maria Giovanna Gandolfi, Fausto Zamparini, Andrea Spinelli, Vittorio Sambri and Carlo Prati
Int. J. Environ. Res. Public Health 2020, 17(23), 8954; https://doi.org/10.3390/ijerph17238954 - 1 Dec 2020
Cited by 34 | Viewed by 5516
Abstract
Dental-care workers operate very close to the patient’s mouth and are at high risk of contamination by SARS-CoV-2. Droplets may be contaminated by patient’s saliva and exhaled breath particles. All asymptomatic patients should be considered as Coronavirus positive. All dental procedures must be [...] Read more.
Dental-care workers operate very close to the patient’s mouth and are at high risk of contamination by SARS-CoV-2. Droplets may be contaminated by patient’s saliva and exhaled breath particles. All asymptomatic patients should be considered as Coronavirus positive. All dental procedures must be revised after positive identification of SARS-Cov-2. Novel recommendations as the use of novel suction cannula designed for fast spray/saliva aspiration, use of Tyvek suits and innovative sprayhoods designed for dental-care worker protections are proposed to prevent virus transmission. New tailored operative and clinical procedures are being currently developed by university dental clinics and hospitals in attempt to reduce risk for dental workers and patients. Full article
(This article belongs to the Special Issue Dental Public Health: Issues, Challenges and Opportunities)
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7 pages, 312 KiB  
Article
Colonization of Dental Unit Waterlines by Helicobacter pylori: Risk of Exposure in Dental Practices
by Monica Giacomuzzi, Carla M. Zotti and Savina Ditommaso
Int. J. Environ. Res. Public Health 2019, 16(16), 2981; https://doi.org/10.3390/ijerph16162981 - 19 Aug 2019
Cited by 8 | Viewed by 3505
Abstract
Dental unit waterlines (DUWLs) can be considered one of the possible routes of H. pylori transmission, although its presence in DUWLs has not yet been investigated thoroughly. The present study aimed to discover the prevalence of H. pylori and oral streptococci (S. [...] Read more.
Dental unit waterlines (DUWLs) can be considered one of the possible routes of H. pylori transmission, although its presence in DUWLs has not yet been investigated thoroughly. The present study aimed to discover the prevalence of H. pylori and oral streptococci (S. oralis and S. mutans) in DUWLs to evaluate the risk of exposure to human pathogens in dental practices. We collected the output water from 60 dental chair units (DCUs) in 26 private dentistry settings in Turin, searching for H. pylori and oral streptococci (OS) DNA, with a polymerase chain reaction (PCR) technique. At the same time, dentists completed a questionnaire about their DCUs, their main activities, the presence of anti-retraction devices, their attitudes about disinfection, etc. No dental chair unit tested was contaminated with H. pylori or S. mutans; only one dental chair was contaminated with S. oralis (1.7%). Considering the results, we can state that: (i) the lack of H. pylori DNA in water samples analyzed, suggests that municipal water is presumably treated with a sufficient chlorine level to inactivate DNA over time; (ii) the aspiration of oral fluids is limited by anti-retraction valves fitted distally to hand pieces; (iii) propidium monoazide qPCR (PMA-qPCR) could be a good technique to investigate and monitor potential environmental sources of infections such as DUWLs. Full article
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15 pages, 8621 KiB  
Article
Detection of Legionella anisa in Water from Hospital Dental Chair Units and Molecular Characterization by Whole-Genome Sequencing
by Giuseppe Fleres, Natacha Couto, Mariette Lokate, Luc W. M. Van der Sluis, Christophe Ginevra, Sophie Jarraud, Ruud H. Deurenberg, John W. Rossen, Silvia García-Cobos and Alex W. Friedrich
Microorganisms 2018, 6(3), 71; https://doi.org/10.3390/microorganisms6030071 - 18 Jul 2018
Cited by 18 | Viewed by 6747
Abstract
This study aims to assess contamination with Legionella spp. in water from dental chair units (DCUs) of a hospital dental ward and to perform its molecular characterization by whole-genome sequencing (WGS). We collect eight water samples (250 mL) from four DCUs (sink and [...] Read more.
This study aims to assess contamination with Legionella spp. in water from dental chair units (DCUs) of a hospital dental ward and to perform its molecular characterization by whole-genome sequencing (WGS). We collect eight water samples (250 mL) from four DCUs (sink and water-syringe). Samples are tested for the presence of Legionella spp. (CFUs/mL) by culturing according to the Nederland Norm (NEN) 6265. Three DCUs are found positive for Legionella anisa, and four isolates are cultured (sink n = 2, water-syringe n = 1; two isolates from the same chair) with 1 × 102 CFU/mL. Whole-genome multi-locus sequence typing (wgMLST) results indicate that all strains belong to the same cluster with two to four allele differences. Classical culture combined with WGS allows the identification of a unique clone of L. anisa in several DCUs in the same hospital dental ward. This may indicate a common contamination source in the dental unit waterlines, which was fixed by replacing the chairs and main pipeline of the unit. Our results reveal tap water contamination in direct contact with patients and the usefulness of WGS to investigate bacterial molecular epidemiology. Full article
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