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Keywords = cyclohexanone monoxygenase

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12 pages, 4337 KB  
Article
Polyelectrolyte Complex Beads by Novel Two-Step Process for Improved Performance of Viable Whole-Cell Baeyer-Villiger Monoxygenase by Immobilization
by Tomáš Krajčovič, Marek Bučko, Alica Vikartovská, Igor Lacík, Lucia Uhelská, Dušan Chorvát, Vilém Neděla, Eva Tihlaříková, Martin Gericke, Thomas Heinze and Peter Gemeiner
Catalysts 2017, 7(11), 353; https://doi.org/10.3390/catal7110353 - 21 Nov 2017
Cited by 12 | Viewed by 6259
Abstract
A novel immobilization matrix for the entrapment of viable whole-cell Baeyer–Villiger monooxygenase was developed. Viable recombinant Escherichia coli cells overexpressing cyclohexanone monooxygenase were entrapped in polyelectrolyte complex beads prepared by a two-step reaction of oppositely-charged polymers including highly defined cellulose sulphate. Immobilized cells [...] Read more.
A novel immobilization matrix for the entrapment of viable whole-cell Baeyer–Villiger monooxygenase was developed. Viable recombinant Escherichia coli cells overexpressing cyclohexanone monooxygenase were entrapped in polyelectrolyte complex beads prepared by a two-step reaction of oppositely-charged polymers including highly defined cellulose sulphate. Immobilized cells exhibited higher operational stability than free cells during 10 repeated cycles of Baeyer–Villiger biooxidations of rac-bicyclo[3.2.0]hept-2-en-6-one to the corresponding lactones (1R,5S)-3-oxabicyclo-[3.3.0]oct-6-en-3-one and (1S,5R)-2-oxabicyclo-[3.3.0]oct-6-en-3-one. The morphology of polyelectrolyte complex beads was characterised by environmental scanning electron microscopy; the spatial distribution of polymers in the beads and cell viability were examined using confocal laser scanning microscopy, and the texture was characterised by the mechanical resistance measurements. Full article
(This article belongs to the Special Issue Immobilized Biocatalysts)
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