Search Results (208)

The infant oral microbiome is a complex and dynamic microbial community that undergoes various transformations during human development. From birth, these microorganisms are modulated by factors such as birth type, nutrition, oral hygiene, hormonal changes, and environmental and socioeconomic conditions. These elements interact continuously, shaping the diversity and stability of the oral microbiome and consequently influencing the oral and general health of individuals. The main objective of this study was to review the literature on the evolution of the oral microbiome at different stages of growth, with special emphasis on the maintenance of dental homeostasis and prevention of pathologies such as caries and periodontitis. A bibliographic review of scientific databases was conducted, focusing on the last decade. In general, oral microbiome dysbiosis increases the risk of oral diseases and systemic conditions. Diet, parental practices, and horizontal transmission of bacteria from caregivers have been shown to modulate and influence the composition and functioning of the infant oral microbiome. Despite these advances, gaps remain in our understanding of the impact of the pediatric oral microbiome on long-term comprehensive health. Therefore, longitudinal research is needed to understand the development of the oral microbiome and its potential role in early prediction, prevention, and treatment of oral and systemic diseases. Full article

Background/Objectives: Official data on the oral health of young children in Romania are limited, especially concerning the societal shifts following the COVID-19 pandemic. This study aimed to compare the oral health status, hygiene habits, diet, and dental care patterns of 6-year-old children in a rural Romanian region before and after the pandemic. Methods: A cross-sectional survey studied two groups of 6-year-olds from rural Transylvania: Group 1 (n = 77), assessed 2018–2020 pre-pandemic, and Group 2 (n = 136), assessed in 2024 post-pandemic. Clinical data used the Decayed, Missing, and Filled Teeth (DMFT) index. Parents completed questionnaires on oral hygiene, diet, dental visits, and pandemic-related topics for Group 2. Chi-square, Student’s t-tests, and a multiple linear regression were used to analyze the data. Results: Post-pandemic, 70% of children brushed twice daily, up from 26%. Despite this, negative outcomes increased: the number of affected teeth rose from 6.8% to 29.6% (p < 0.001), sugar intake increased, and dental visits became more reactive, with pain being the main reason for 61% of post-pandemic visits, compared to 17% pre-pandemic. Conclusions: This study reveals a significant oral health paradox: despite a three-fold increase in recommended toothbrushing, caries experience in permanent teeth increased four-fold. This outcome was driven by a high-risk environment of more cariogenic diets and reduced preventive care, with lower maternal education also identified as a key independent risk factor. These findings show that promoting hygiene alone is insufficient, underscoring the urgent need for public health programs that integrate nutritional counseling, improve access to preventive care, and address underlying socioeconomic disparities. Full article

Dental caries is now recognised as a multifactorial disease shaped by complex interactions among genetic, epigenetic, microbiological, environmental, and social factors. This narrative review synthesises recent findings on the influence of genetic and epigenetic factors on caries susceptibility, exploring implications for personalised prevention strategies, including novel vaccine approaches. Numerous gene polymorphisms in pathways related to enamel formation, saliva composition, immune response, and taste perception have been linked to increased caries risk, with some effects modulated by sex and tooth-specific factors. Early-life environmental exposures (diet, tobacco, and antibiotic use) have been demonstrated to further alter risk through epigenetic modifications such as DNA methylation, microRNA regulation, and histone changes. The recognition of this landscape of inherited and acquired vulnerabilities has given rise to interest in innovative preventive measures. In particular, anti-caries vaccines targeting Streptococcus mutans are being developed using protein subunits, DNA constructs, and even plant-based antigen production. Notwithstanding the challenges that still need to be overcome—chiefly the achievement of robust mucosal immunity, the assurance of safety, and the enhancement of production—these vaccines are proving to be a promising addition to traditional oral hygiene and fluoride measures. The integration of genetic and epigenetic insights with immunological advances has the potential to facilitate the development of more effective, personalised interventions to prevent dental caries. Full article

Introduction: Dental caries and molar–incisor hypomineralisation (MIH) are prevalent conditions affecting children’s oral health, with functional, aesthetic, and psychosocial implications. In Spain, previous studies have highlighted geographic and sociodemographic disparities in their distribution, particularly among rural and migrant populations. Objective: To characterise oral health status, in terms of caries and MIH, among 6–7-year-old children from the towns of Palos de la Frontera, Mazagón, and San Bartolomé. Methods: A cross-sectional study was conducted involving 229 children recruited from public primary schools. Sociodemographic, anthropometric, and behavioural data were collected through clinical examination and interview. Statistical analysis included univariate and multivariate logistic regression. The study protocol was approved by the Ethics Committee of Huelva. Results: The prevalence of caries (DMFT ≥ 1) was 53.3%, with mean DMFT and dft indices of 1.78 and 0.31, respectively. MIH affected 32.8% of the cohort, with a predominance in the first permanent molars (teeth 36 and 26). Multivariate analysis identified independent predictors of caries: African (OR = 7.47; 95% CI: 2.84–23.8) and European (OR = 4.56; 95% CI: 1.26–22.3) parental origin, poor oral hygiene (OR = 3.07; 95% CI: 1.60–6.03), and the presence of MIH (OR = 3.20; 95% CI: 1.64–6.42). The municipality of San Bartolomé was associated with a higher risk of MIH (OR = 2.90; 95% CI: 1.21–7.45). Conclusions: The high prevalence of caries and MIH in the Condado-Campiña district, exceeding national averages, reflects oral health inequities linked to social determinants (migrant origin, locality) and clinical factors (MIH, oral hygiene). Targeted preventive interventions are urgently needed in high-risk populations, including culturally tailored education and policies ensuring equitable access to dental care services. Full article

Child and adolescent health plays a critical role in shaping future public health and intergenerational outcomes. In China, rising rates of myopia, obesity, mental health issues, and other common conditions highlight the need for continuous monitoring. Since 2016, the Chinese National Surveillance on Students’ Common Diseases and Risk Factors (CNSSCDRF) has provided comprehensive, nationwide data on student health. By 2022, the system had expanded to nearly all counties, tracking key indicators such as vision problems, overweight/obesity, dental caries, and health-risk behaviors across multiple administrative levels. This review outlines the surveillance methodology, including sampling, data collection, and analysis. Findings have directly informed school health policies and technical guidelines, supporting national goals such as those in the Healthy China 2030 Plan. Full article

Objectives: The present study aims to compare the prevalence of dental caries between children with special needs (CSN) and children without special health care needs (CWSCN), and additionally, this study explores the association between body mass index (BMI) and dental caries in both groups. Methods: A cross-sectional descriptive study was conducted. A total of 773 children were selected using the two-stage random sampling method (257 CSN and 516 CWSCN). The World Health Organization criteria was used to diagnose dental caries. BMI was determined by using height and weight measurements. Multivariable logistic regression was used to determine the relationships between dental caries prevalence (yes/no) and independent variables. Result: Special needs children had a 2.87 (95% CI: 1.56–4.03, p = 0.001) times higher risk of caries compared with CWSCN. Female children had a 1.76 (95% CI: 1.52–3.24, p = 0.024) times higher risk of caries than male children. Children who consume sugar frequently were 2.03 (95% CI: 1.73–4.08, p = 0.001) times more likely to have caries. Children with obesity were 2.15 (95% CI: 1.81–4.79, p = 0.001) times more likely to have caries compared with normal-weight children. Children who used non-fluoridated toothpaste had a 1.92 times (95% CI: 1.68–4.19, p = 0.031) higher risk of caries compared with children who used fluoridated toothpaste. Conclusions: The present study highlights the higher prevalence of dental caries among CSN compared with CWSCN and identifies several significant risk factors, including gender, parental education, sugar consumption, obesity, and the use of non-fluoridated toothpaste. Full article

Background/Objectives: Dental caries (DCs) and type 2 diabetes share common risk factors. Dental caries risk in type 2 diabetics (T2DM) shows contradictory results. The aim of this study was to determine if there is a difference in DC prevalence in adults with and without T2DM and whether body mass index (BMI) classes or glycated hemoglobin (HbA1c) levels interfere in that difference. Methods: A total of 666 adults (n(T2DM) = 343; n(nT2DM) = 323), from Espinho Primary Health Care Center, were interviewed by calibrated observers. Data from clinical records were collected and oral health status was registered using WHO criteria. Inference analysis was conducted using non-parametric tests (α = 0.05). Results: A similar caries prevalence was found between the T2DM (98.2%) and nT2DM (98.8%) groups, with the T2DM group showing significantly higher tooth loss (p < 0.001), higher caries experience rerted as mean ± sd (17.7 ± 8.3 vs. 15.9 ± 7.8, p = 0.005), fewer decayed teeth (p < 0.001) and filled teeth (p = 0.016) compared to nT2DM. The most frequently identified comorbidity was hypertension (53.6%). Tobacco use (12.9%) was lower in T2DM (p < 0.001). The restorative and treatment indices indicated a significantly higher proportion of use of oral care services (p < 0.001) in T2DM individuals. The prevalence of the higher classes of BMI indicative of pre-obesity or obesity shows significant differences (p < 0.001). The differences found in the DMFT or each of its components for the prevalence or for the mean in HbA1c control were not statistically significant (p = 0.368, and 0.524, respectively). Conclusions: Adults with T2DM and higher BMI classes could be associated with a greater prevalence of DCs. The glycemic control of T2DM does not significantly influence DMFT score or each of its components. Full article

Objectives: Dental caries remains a prevalent issue in pediatric dentistry, necessitating restorative materials that not only repair decay but also provide protective benefits. Fluoride-releasing restorative materials have a key function in preventing recurrent caries by inhibiting bacterial activity and promoting remineralization. The objective of this study was to examine fluoride release from three pediatric dental restorative materials—Riva Light Cure HV, Fuji IX GP Fast, and the Cention Forte Filling Material—under different pH and temperature conditions. Methods: Specimens (10 mm diameter and 2 mm thickness; n = 3 for each material) were prepared according to the manufacturers’ instructions; immersed in buffer solutions at pH 4.8, 6.8, and 8.8; and stored at 37 °C and 44 °C. Fluoride release was quantified using ion chromatography at three time points (1 day, 7 days, and 28 days). Results: The data revealed that fluoride release was significantly influenced by pH, temperature, and time (p < 0.05). Riva Light Cure HV exhibited the highest release, particularly in acidic conditions (pH 4.8), reaching 40.14 mg/L at 44 °C after 28 days. The Cention Forte Filling Material and Fuji IX GP Fast also showed increased release over time, but with lower cumulative concentrations. Higher temperatures generally enhanced fluoride diffusion across all materials. Conclusions: These findings emphasize the pivotal impact of environmental factors in fluoride release dynamics. Riva Light Cure HV demonstrated superior fluoride release, particularly in acidic environments, suggesting its potential for high-caries-risk pediatric patients. These insights can inform the selection of restorative materials in pediatric dentistry, optimizing caries prevention strategies. Full article

Staphylococcus aureus, an opportunistic bacterium found in the oral cavity, has been reported as a causative agent of infective endocarditis and pneumonia. Salt is an essential mineral for cell maintenance in the human body. This study was conducted to clarify how salt affects the formation of biofilms by S. aureus and Streptococcus mutans, pathogens implicated in dental caries. Bacteria were cultivated with various concentrations of NaCl on a 96-well microtiter plate in tryptic soy broth with 0.25% sucrose or 0.25% glucose (TSBs and TSBg, respectively) for 16 h. The effects of glucosyltransferase in S. mutans membrane vesicles (MVs) and extracellular DNA during biofilm formation were also analyzed. S. aureus biofilms were induced by 0.004–0.25 M NaCl but not by NaCl at concentrations greater than 0.25 M in TSBs. The mixed S. aureus and S. mutans biofilms gradually grew and were constructed by dead cells in a NaCl concentration-dependent manner in both TSBs and TSBg. Moreover, biofilms were slightly induced by glucan generation mediated by the glucosyltransferases in MVs under high-salinity conditions. The formation of mixed-species S. aureus and S. mutans biofilms increased in the presence of both extracellular DNA and MVs. Therefore, extracellular DNA, MVs, and dead cells are factors that promote S. aureus biofilm formation under harsh conditions containing NaCl. The sugar (sucrose and glucose) ingestion-induced S. mutans biofilm may be a risk factor for infection by opportunistic pathogens such as S. aureus in individuals who consume food and drinks containing high concentrations of salt. Full article

Background: Guidelines suggest that, in chronic non-cancer pain (CNCP) management, non-opioid modalities should be prioritized, as there are negative consequences related to opioid use. There is a dearth of literature elucidating the risk factors for initiating opioid use amongst veterans with CNCP. Methods: Chart review on 193 patients with a new referral at a local US Department of Veterans Affairs (VA) pain clinic. Patients were required to have CNCP and not be taking opioid medication at the time of referral. The review started on 1 January 2014 and covered the year following. Data were analyzed via stepwise multiple logistic regression using Statistical Analysis System (SAS) software (Version 9.4; SAS institute Inc., Cary, NC, USA). Results: A total of 37 veterans (19%) received a new opioid prescription in the year following initial encounters at the pain clinic for CNCP. A history of substance use was associated with lower odds of receiving an opioid prescription. In contrast, being employed was associated with higher odds of receiving an opioid prescription. Conclusions: Amongst veterans treated for CNCP in a VA pain clinic, a history of substance use and a presence of vocation within the past year prior to presentation are variables associated with the initiation of opioids. Future studies to further elucidate the predictors of opioid prescriptions for CNCP are warranted. Full article

Background/Objectives: Parental attitudes play a crucial role in shaping children’s oral health habits and preventing dental diseases. This study aimed to explore the theoretical pathways through which parental behavior and attitudes toward child oral health can influence the dental caries experience as measured by the dmf-t index in preschool children in Lithuania. Methods: A cross-sectional study was conducted involving 302 children aged 4–7 years and their parents (262 mothers). Parental attitudes were assessed using the Parental Attitudes Towards Child Oral Health (PACOH) scale. For the children, the following variables were considered: sex, age, dental caries experience (dmf-t index in the primary dentition), oral hygiene index (Silness–Löe Plaque Index), toothbrushing frequency, and parental assistance with toothbrushing. Structural Equation Modeling (SEM) was applied for the data analysis. Results: The main path through which parental attitudes towards child oral health influenced the dmf-t index was via toothbrushing frequency (β = −0.17) or parental assistance with toothbrushing (β = 0.24). These factors were then linked to the oral hygiene index (β = 0.20 and β = −0.47, respectively), which ultimately influenced dmf-t (β = 0.52). The parents’ attitudes and toothbrushing frequency per se had no significant effect on children’s dmf-t (β = −0.06 and β = −0.04, respectively). The final model met all goodness-of-fit criteria: Chi-square test p = 0.211, Incremental Fit Index IFI = 0.994, Tucker–Lewis Index TLI = 0.982, Comparative Fit Index CFI = 0.994, and Root Mean Square Error of Approximation RMSEA = 0.038. Conclusions: Findings from this study demonstrate that parents play a significant role in determining children’s oral health. Regular toothbrushing, parental assistance with toothbrushing, and good oral hygiene are critical factors linking parents’ oral health-related attitudes to a child’s experience of early caries. Identifying the associations between dental caries risk factors helps plan interventions. Full article

Background: Third molar (M3) removal is considered one of the most frequent oral surgical procedures worldwide. Indications for extraction include both prophylactic and therapeutic reasons. However, this does not come without complications, and despite the widespread practice, there is no consensus on whether prophylactic M3 extraction is more beneficial than conservative management. Aims: The aim of this systematic review is to highlight and compare the main differences and outcomes between prophylactic and therapeutic removal of third molars with conservative treatment and observation. Several factors have been considered such as post-surgical infection risks and complications, hospitalization indications, economic factors and periodontal health of adjacent teeth. Methods: A literature review and meta-analysis were conducted, which comprises studies describing the incidence of postoperative complications, the periodontal status of the second molar (M2), the prevalence of caries, and the economic aspects of the M3 removal. Periodontal parameters of the adjacent teeth such as periodontal pocket depth (PPD) and clinical attachment level (CAL), as well as inferior alveolar nerve (IAN) damage and post-operative inflammatory complications such as bacteremia, were considered. Finally, hospitalization and the economic burden of this procedure were also stated. Results: Third molar retention is associated with increased periodontal disease such as PPD and accumulation of plaque to the adjacent teeth, as well as risk of caries. Contrarily, prophylactic M3 extraction is often linked to unnecessary morbidity and costs, such as risk of bacteremia, trismus, postoperative pain, IAN damage, and sometimes the need for hospitalization. From an economic aspect, this frequent procedure is mostly associated with higher direct and indirect costs, which can exceed the amount of EUR 1000 per patient without hospitalization. Conclusions: This review tried to determine whether the M3 observation and retention can be more beneficial than M3 extraction, after examining certain parameters. Findings indicate that unnecessary morbidity and costs can be attributed to third molar extraction, with postoperative complications such as pain and trismus and sometimes the need for hospitalization. Transient bacteremia also accompanies third molar removal. Full article

Objective: This systematic review evaluates the evidence on factors affecting fluoride release from compomer restorative materials to provide clinicians with insights for optimizing their use in caries prevention. Methods: In February 2025, an extensive digital search was conducted across reputable databases such as PubMed, Web of Science, and Scopus. The search utilized carefully chosen keywords: “fluoride release” AND “compomer” and followed the PRISMA guidelines. Initially, 287 articles were identified, but after applying the inclusion criteria, 34 studies were selected for review. Results: This review found that fluoride release from compomers follows an initial burst phase before stabilizing at lower levels. Fifteen studies proved that compomers release less fluoride than glass ionomer cements but more than composite resins, as concluded from six studies. The release rate is significantly influenced by pH, with acidic conditions enhancing fluoride diffusion. Some studies also highlighted the potential for fluoride recharge through external applications such as toothpaste or varnish. Conclusion: Compomer restorative materials offer a steady, moderate fluoride release that supports caries prevention. Their effectiveness is enhanced in acidic environments, supporting their use in high-risk patients. Full article

Improving children’s oral health is a national priority. Parental knowledge and self-efficacy influence children’s health behaviors; however, the relationship between parental oral health-related knowledge, self-efficacy, child oral health-related quality of life (OHQoL), and dental caries remains unclear, especially among low-income, ethnically diverse U.S. families. This study assesses the relationship between parental oral health-related knowledge, self-efficacy, child dental caries, and OHQoL. This cross-sectional seco ndary analysis uses baseline data from a school-based cluster-randomized controlled trial among children (kindergarten–second grade). Dental assessments were conducted using the International Caries Detection and Assessment System (ICDAS) on site in schools at baseline (n = 34 schools, n = 1084 consenting parent–child dyads). Child OHQoL, parental knowledge, and self-efficacy were measured using validated self-report surveys. Associations between exposures and outcomes (child dental caries prevalence, child OHQoL) were evaluated by univariate and multivariable logistic and linear regressions, respectively. After adjusting for covariates, higher parental self-efficacy was associated with lower child OHQoL (lower score indicates higher quality of life) [beta = −0.16, 95% CI: −0.24, −0.09, p < 0.01] and lower odds of active dental caries [Adj OR: 0.95, 95% CI: 0.9, 0.99, p = 0.02]. No significant associations were noted for parental knowledge. These findings can inform future research, understanding how parental psychosocial factors influence dental caries prevention behaviors and risk, and inform interventions for children. Full article

Background: In Mexico, tooth decay is common among children and is on the rise. A strong relationship exists between behavioral, socioeconomic, demographic, and biological factors and the severity and development of dental caries. Objective: The present study analyzed the risk factors contributing to dental caries in a Mexican pediatric population. Materials and Methods: This study employed a cross-sectional design. One hundred fifty-one children were evaluated after providing their assent and the informed consent of their legal guardians. The study subjects recruited underwent dental assessments, dietary habits, and physical activity examinations. Results: Only 36 children were classified as overweight or obese, while 121 children had a high quality of life related to oral health. Most of the children showed deficient eating and physical activity habits. Additionally, nearly half displayed severe dental carie lesions. The occurrence of caries was linked to both age and tooth brushing habits. The median of teeth with initial caries lesions was higher among female children. Furthermore, parental education was associated with the presence of fluorosis and the frequency of tooth brushing. Conclusion: Our study revealed deficient eating habits and a high prevalence of dental caries among children aged 6–12. Additionally, parents and guardians demonstrated insufficient knowledge about general oral health issues, the risk factors for caries, and their potential consequences, which means a need for both preventive and corrective treatments. This highlights the need for strategies focused on improving oral health care and prevention. Full article