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Keywords = azidolysozyme

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10 pages, 6783 KB  
Article
Cross-Correlated Motions in Azidolysozyme
by Seyedeh Maryam Salehi and Markus Meuwly
Molecules 2022, 27(3), 839; https://doi.org/10.3390/molecules27030839 - 27 Jan 2022
Cited by 7 | Viewed by 3769
Abstract
The changes in the local and global dynamics of azide-labelled lysozyme compared with that of the wild type protein are quantitatively assessed for all alanine residues along the polypeptide chain. Although attaching -N3 to alanine residues has been considered to be a [...] Read more.
The changes in the local and global dynamics of azide-labelled lysozyme compared with that of the wild type protein are quantitatively assessed for all alanine residues along the polypeptide chain. Although attaching -N3 to alanine residues has been considered to be a minimally invasive change in the protein it is found that depending on the location of the alanine residue, the local and global changes in the dynamics differ. For Ala92, the change in the cross-correlated motions are minimal, whereas attaching -N3 to Ala90 leads to pronounced differences in the local and global correlations as quantified by the cross-correlation coefficients of the Cα atoms. We also demonstrate that the spectral region of the asymmetric azide stretch distinguishes between alanine attachment sites, whereas changes in the low frequency, far-infrared region are less characteristic. Full article
(This article belongs to the Special Issue Women in Physical Chemistry)
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