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Keywords = Tanypus

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12 pages, 4080 KB  
Article
Complete Mitochondrial Genome of Tanypus chinensis and Tanypus kraatzi (Diptera: Chironomidae): Characterization and Phylogenetic Implications
by Shaobo Gao, Chengyan Wang, Yaning Tang, Yuzhen Zhang, Xinyu Ge, Jiwei Zhang and Wenbin Liu
Genes 2024, 15(10), 1281; https://doi.org/10.3390/genes15101281 - 29 Sep 2024
Cited by 7 | Viewed by 1826
Abstract
Background: Chironomidae occupy a pivotal position within global aquatic ecosystems. The unique structural attributes of the mitochondrial genome provide profound insights and compelling evidence, underpinning the morphological classification of organisms and substantially advancing our understanding of the phylogenetic relationships within Chironomidae. Results: We [...] Read more.
Background: Chironomidae occupy a pivotal position within global aquatic ecosystems. The unique structural attributes of the mitochondrial genome provide profound insights and compelling evidence, underpinning the morphological classification of organisms and substantially advancing our understanding of the phylogenetic relationships within Chironomidae. Results: We have meticulously sequenced, assembled, and annotated the mitogenomes of Tanypus chinensis (Wang, 1994) and Tanypus kraatzi (Kieffer, 1912), incorporating an additional 25 previously published mitogenomes into our comprehensive analysis. This extensive dataset enables us to delve deeper into the intricate characteristics and nuances of these mitogenomes, facilitating a more nuanced understanding of their genetic makeup. Conclusions: The genomic nucleotide composition of T. kraatzi was 39.10% A, 36.51% T, 14.33% C, and 10.06% G, with a total length of 1508 bp. The genomic nucleotide composition of T. chinensis was 39.61% A, 36.27% T, 14.55% C, and 9.57% G, with a total length of 1503 bp. This significant enrichment of the chironomid mitogenome library establishes a novel foundation for further exploration in the realm of phylogenetics. Full article
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20 pages, 9173 KB  
Article
Improved Chironomid Barcode Database Enhances Identification of Water Mite Dietary Content
by Adrian A. Vasquez, Brittany L. Bonnici, Safia Haniya Yusuf, Janiel I. Cruz, Patrick L. Hudson and Jeffrey L. Ram
Diversity 2022, 14(2), 65; https://doi.org/10.3390/d14020065 - 19 Jan 2022
Cited by 8 | Viewed by 4733
Abstract
Chironomids are one of the most biodiverse and abundant members of freshwater ecosystems. They are a food source for many organisms, including fish and water mites. The accurate identification of chironomids is essential for many applications in ecological research, including determining which chironomid [...] Read more.
Chironomids are one of the most biodiverse and abundant members of freshwater ecosystems. They are a food source for many organisms, including fish and water mites. The accurate identification of chironomids is essential for many applications in ecological research, including determining which chironomid species are present in the diets of diverse predators. Larval and adult chironomids from diverse habitats, including lakes, rivers, inland gardens, coastal vegetation, and nearshore habitats of the Great Lakes, were collected from 2012 to 2019. After morphological identification of chironomids, DNA was extracted and cytochrome oxidase I (COI) barcodes were PCR amplified and sequenced. Here we describe an analysis of biodiverse adult and larval chironomids in the Great Lakes region of North America based on new collections to improve chironomid identification by curating a chironomid DNA barcode database, thereby expanding the diversity and taxonomic specificity of DNA reference libraries for the Chironomidae family. In addition to reporting many novel chironomid DNA barcodes, we demonstrate here the use of this chironomid COI barcode database to improve the identification of DNA barcodes of prey in the liquefied diets of water mites. The species identifications of the COI barcodes of chironomids ingested by Lebertia davidcooki and L. quinquemaculosa are more diverse for L. davidcooki and include Parachironomus abortivus, Cryptochironomus ponderosus. Parachironomus tenuicaudatus, Glyptotendipes senilis, Dicrotendipes modestus, Chironomus riparius, Chironomus entis/plumosus, Chironomus maturus, Chironomus crassicaudatus, Endochironomus subtendens, Cricotopus sylvestris, Cricotopus festivellus, Orthocladius obumbratus, Tanypus punctipennis, Rheotanytarsus exiguus gr., and Paratanytarsus nr. bituberculatus. Full article
(This article belongs to the Special Issue Aquatic Organisms Research with DNA Barcodes)
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