Search Results (5)

Trigeminal neuralgia is a chronic pain disorder due to neuronal damage. The present study was designed to investigate the effect of 7,8-dimethoxy coumarin (DMC) in a rat model of trigeminal neuralgia. The neuropathic pain was induced by the single endoneural injection of tumor necrosis factor-alpha (TNF-α; 0.1 μL: stock 10 pg/mL) in the rat trigeminal nerve. The DMC (100 and 200 mg/kg) and carbamazepine (100 mg/kg) were administered orally for 10 consecutive days from the 5th day of TNF-α injection. The battery of behavioral tests, i.e., acetone drop and Von Frey filament test, was performed to assess the degree of thermal and mechanical allodynia on 0, 1st, 7th, and 14th days. In addition, the biochemical tests, i.e., total protein, thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH), and TNF-α, were also performed in trigeminal nerve tissue. Furthermore, TNF-α-induced neuronal histopathological changes were also evaluated by the eosin and hematoxylin staining method. The administration of DMC was shown to demonstrate the significant (p < 0.05) reversal of TNF-α-induced percentage reduction of thermal and mechanical sensitivity, along with a rise in TBARS and TNF-α and a decrease in GSH levels. Further, DMC also attenuates the histopathological changes. It may be concluded that DMC may be a potential therapeutic agent for the management of trigeminal neuralgia disorders. Full article

Forest fire is one of the dominant disturbances in the forests of Heilongjiang Province, China, and is one of the most rapid response predictors that indicate the impact of climate change on forests. This study calculated the Canadian FWI (Fire Weather Index) and its components from meteorological record over past years, and a linear model was built from the monthly mean FWI and monthly fire numbers. The significance test showed that fire numbers and FWI had a very pronounced correlation, and monthly mean FWI was suitable for predicting the monthly fire numbers in this region. Then FWI and its components were calculated from the SRES (IPCC Special Report on Emission Scenarios) A2 and B2 climatic scenarios, and the linear model was rebuilt to be suitable for the climatic scenarios. The results indicated that fire numbers would increase by 2.98–129.97% and −2.86–103.30% in the A2 and B2 climatic scenarios during 2020–2090, respectively. The monthly variation tendency of the FWI components is similar in the A2 and B2 climatic scenarios. The increasing fire risk is uneven across months in these two climatic scenarios. The monthly analysis showed that the FFMC (Fine Fuel Moisture Code) would increase dramatically in summer, and the decreasing precipitation in summer would contribute greatly to this tendency. The FWI would increase rapidly from the spring fire season to the autumn fire season, and the FWI would have the most rapid increase in speed in the spring fire season. DMC (Duff Moisture Code) and DC (Drought Code) have relatively balanced rates of increasing from spring to autumn. The change in the FWI in this region is uneven in space as well. In early 21st century, the FWI of the north of Heilongjiang Province would increase more rapidly than the south, whereas the FWI of the middle and south of Heilongjiang Province would gradually catch up with the increasing speed of the north from the middle of 21st century. The changes in the FWI across seasons and space would influence the fire management policy in this region, and the increasing fire numbers and variations in the FWI scross season and space suggest that suitable development of the management of fire sources and forest fuel should be conducted. Full article

Biodiesel, an alternative to traditional diesel, is essential for the sustainability of long-term energy supplies and often synthesized through a non-alcoholic route called interesterification. The described synthesis method facilitates the modification of oil and fat by exchanging acyl radical groups between triglyceride and alcoholic acid (alcoholysis), fat (acidolysis), or ester (transesterification). Therefore, this research aimed to determine the effect of the reactant ratio between crude palm oil (CPO) and dimethyl carbonate (DMC), along with the use of an eco-enzyme catalyst, on biodiesel characteristics. The CPO:DMC ratio was 1:1.5, 1:2, 1:2.5, and 1:3, while the immobilized eco-enzyme catalyst was 2%, 3%, 4%, 5%, and 6% of CPO mass. The results showed that interesterification with a 1:3 reactant ratio using a 4%wt catalyst was the best procedure, producing biodiesel yield of 73.65%, density of 0.860 g/mL, viscosity of 4.63 mm²/s (cSt), flash point of 113 °C, calorific value of 34.454 MJ/kg, and cetane number of 70.6%. Full article

Fixing the genomic composition and multiplication through true potato seed (TPS) is an important challenge in autotetraploid potato. Disrupted meiotic cDNA (DMC1) is a meiotic gene that plays a central role in DNA recombination through crossing over in meiosis. Using the Arabidopsis DMC1 (AtDMC1) gene sequence, we retrieved Solanum tuberosum DMC1(StDMC1) from the diploid potato genome, and subsequently, sense and antisense regions of the StDMC1 gene were amplified in potato cv. Kufri Jyoti. The sense and antisense fragments were confirmed by Sanger-sequencing and cloned in the pRI101 vector. Agrobacterium-mediated transformation of the RNAi construct resulted in 44% transformation efficiency, and a total of 137 mutant lines were obtained. These mutant lines were further validated through pollen viability testing, and selected lines were used for gene expression analysis. The acetocarmine-based pollen staining showed reduced pollen viability ranging from 14 to 21% in four DMC1 mutant lines (DMC4-37, DMC4-41, DMC6-20, and DMC6-21), as compared to the Kufri Jyoti control plants, which on average exhibited 78% pollen viability. The phenotypic data was supported by the reduced expression of the StDMC1 gene in these four mutant lines compared to the control Kufri Jyoti. The results confirmed the generation of StDMC1 knockdown lines. This is the first report of StDMC1 mutant line generation in tetraploid potatoes and will be a step forward in generating non-recombinant mutants through sexual reproduction in potatoes. Full article

The Laohahe basin is one of the typical semi-arid regions in Northeast China. Quantitatively estimating the contributions of human intervention and climatic variability on runoff changes in this region will help enhance the understanding of local hydrological mechanisms and provide an effective reference for water resources planning and management in other semi-arid regions of the world. The non-parametric Mann–Kendall test was used to analyze the temporal trends of annual precipitation, potential evapotranspiration (PET), and runoff in the whole Laohahe basin and its three sub-basins from 1964 to 2015. The annual runoff showed a decreasing trend in each sub-basin. The change-points of annual runoff detected by Pettitt’s test and residual analysis based on double mass curves (RA-DMC) are 1979 and 1998, and the baseline period and change period of each basin are 1964–1979 and 1980–2015. The RA-DMC method and the VIC model were used to quantitatively evaluate the contributions of human intervention and climatic variability to runoff change, which vary in time and space over the past 52 years. The contributions of human intervention to runoff reduction during 1980–2015 was more than 80%. On a multi-decadal temporal scale, human intervention had a stronger impact on runoff during 1980–1989, 2000–2009, and 2010–2015. The influence of human intervention on runoff reduction is gradually increasing in the 21st century. Besides, human intervention has a greater impact in dry years than in wet years. The increase of cropland area leads to a significant increase in irrigation area, which further leads to an increase in the demand for agricultural water, which is also the main reason for the sharp reduction of runoff in the Laohahe basin. Full article