Search Results (8)

Potato is one of the most consumed crops worldwide. One strategy aimed at pathogen and pest management is the cultivation of resistant varieties. In this study, 352 genotypes from the Kazakhstan potato germplasm collection were screened for the presence of loci for resistance to the most harmful pathogens and pests using 30 DNA markers. ‘Rucheek’ and ‘Spiridon’, among the top global varieties, and ‘Janaisan’ and ‘Fedor’, among the top local varieties, were identified as bearing the most resistance loci in the collection, with at least 14 markers each. The specimens carrying the genes Ryadg (ADG1, ADG2), Nb (SPUD237), Ns (SC811, CP16), Rpi-phu1 (GP94), and GroV1 (X02), which demonstrate confirmed resistance to PVY, PVA, PVX, PVS, Phytophthora infestans, and Globodera rostochiensis, were most frequently found in the collection. Loci for resistance to Synchytrium endobioticum and Globodera pallida were less introgressed into the Kazakhstani cultivars and were almost absent in the germplasm collection. The less abundant loci found in the current potato collection were Ryadg (RysC3), Gro1-4 (Gro1-4), and Rladg (RGASC₈₅₀). Screening of the potato collection showed that the markers SPUD237, CP60, 45/X1, and CT214 generated additional polymorphic amplicons, while markers Nl25, C237, GP179, and GP122₇₁₈ were less applicable in robust large-scale screening. The results from this study may greatly contribute to marker-assisted selection and gene pyramiding strategies aimed at developing new potato varieties with multiple resistance to biotic stress Full article

In the future, there will be more and more abandoned oil-gas wells with the exploitation of onshore oilfield resources. However, the large height difference in abandoned oil-gas wells can be used as building blocks for gravity power generation, thus maximizing the economic value of abandoned oil-gas wells. In this study, a scheme of gravity power generation by virtue of the spud-in casing depth of oil-gas wells is proposed, and a gravity power generation model based on abandoned oil-gas wells is established. The parameters and economic benefits of gravity energy storage are calculated for oil-gas wells in the Huabei oilfield, the Daqing oilfield, and the Xinjiang oilfield. It is shown that the power density and discharge time of the gravity energy storage system in abandoned oil-gas wells are suitable for distributed power generation. In addition, the fast response characteristics of energy storage in abandoned oil-gas wells are verified, which makes the system suitable for correcting continuous and sudden frequency and voltage changes in the power grid but not suitable for energy arbitrage under a high number of annual cycles. Furthermore, the leveling cost of storage of the gravity system in abandoned oil-gas wells is more economical with the high number of annual cycles. The analysis of this work provides a significant investigation of the feasibility of gravity power generation by using abandoned oil-gas wells. Full article

During drilling in deep shale gas reservoirs, drilling fluid losses, hole wall collapses, and additional problems occur frequently due to the development of natural fractures in the shale formation, resulting in a high number of engineering accidents such as drilling fluid leaks, sticking, mud packings, and buried drilling tools. Moreover, the horizontal section of horizontal well is long (about 1500 m), and the problems of friction, rock carrying, and reservoir pollution are extremely prominent. The performance of drilling fluids directly affects drilling efficiency, the rate of engineering accidents, and the reservoir protection effect. In order to overcome the problems of high filtration in deep shale formations, collapse of borehole walls, sticking of pipes, mud inclusions, etc., optimization studies of water-based drilling fluid systems have been conducted with the primary purpose of controlling the rheology and water loss of drilling fluid. The experimental evaluation of the adsorption characteristics of “KCl + polyamine” anti-collapse inhibitor on the surface of clay particles and its influence on the morphology of bentonite was carried out, and the mechanism of inhibiting clay mineral hydration expansion was discussed. The idea of controlling the rheology and water loss of drilling fluid with high temperature resistant modified starch and strengthening the inhibition performance of drilling fluid with “KCl + polyamine” was put forward, and a high temperature-resistant modified starch polyamine anti-sloughing drilling fluid system with stable performance and strong plugging and strong inhibition was optimized. The temperature resistance of the optimized water-based drilling fluid system can reach 180 °C. Applied to on-site drilling of deep shale gas horizontal wells, it effectively reduces the rate of complex accidents such as sticking, mud bagging, and reaming that occur when resistance is encountered during shale formation drilling. The time for a single well to trip when encountering resistance decreases from 2–3 d in the early stages to 3–10 h. The re-use rate of the second spudded slurry is 100 percent, significantly reducing the rate of complex drilling accidents and saving drilling costs. It firmly supports the optimal and rapid construction of deep shale gas horizontal wells. Full article

The application of geostatistics in seismic inversion techniques has been proven somewhat reliable in the delineation of reservoir properties and has recently attracted the attention of many geoscientists. However, there are cases where its prediction returned negative results after drilling. In this research, we re-evaluated a reservoir in Inas Field, whose geostatistical inversion result wrongly predicted sand continuity, resulting in the spudding of a dry hole. When a geostatistical seismic inversion is successfully applied, it provides an increase in seismic resolution and aids the prediction of sand continuity. Although this method relies more on the statistical data from a well because of the limitation of the seismic data in resolving thin geologic features, the spatial variation of reservoir parameters still depends on seismic data, which often have poor resolution quality. Therefore, to investigate the impact of bandlimited data on the geostatistical inversion, we harmonically extended the seismic bandwidth by applying a sparse-layer spectral inversion algorithm to the data. This algorithm increased the seismic data bandwidth from 80 Hz to 180 Hz, and its tuning thickness reduced from 32 m to 10 m at the reservoir interval. The resultant broadband (180 Hz), as well as the original seismic (narrowband of 80 Hz) data, were both used as input to build two separate geostatistical prediction models, respectively. Twenty (20) realizations of these models were generated, ranked into P10, P50, and P90, and the best case was selected for interpretation. These realizations were used to characterize the reservoir lithofacies distribution. When compared, the result of the broadband inversion, facies and sand distribution model showed that the reservoir facies changed towards the location of the dry well. The broadband geostatistical inversion efficiently improved the reservoir characterization process by not only producing an accurate estimation of the lateral extent of the reservoir heterogeneities but also generating outcomes that help us understand why other geostatistical inversion analyses of the target reservoir were misleading. Contrary to the popular assumption, it was discovered that the tuning effects of bandlimited data could affect the result of a geostatistical inversion and result in wrong facies predictions. Full article

Potato (Solanum tuberosum L.) is the most important vegetable crop globally and is very susceptible to high ambient temperatures. Since heat stress causes the accumulation of reactive oxygen species (ROS), investigations regarding major enzymatic components of the antioxidative system are of the essence. Superoxide dismutases (SODs) represent the first line of defense against ROS but detailed in silico analysis and characterization of the potato SOD gene family have not been performed thus far. We have analyzed eight functional SOD genes, three StCuZnSODs, one StMnSOD, and four StFeSODs, annotated in the updated version of potato genome (Spud DB DM v6.1). The StSOD genes and their respective proteins were analyzed in silico to determine the exon-intron organization, splice variants, cis-regulatory promoter elements, conserved domains, signals for subcellular targeting, 3D-structures, and phylogenetic relations. Quantitative PCR analysis revealed higher induction of StCuZnSODs (the major potato SODs) and StFeSOD3 in thermotolerant cultivar Désirée than in thermosensitive Agria and Kennebec during long-term exposure to elevated temperature. StMnSOD was constitutively expressed, while expression of StFeSODs was cultivar-dependent. The effects of salicylic acid (10⁻⁵ M) on StSODs expression were minor. Our results provide the basis for further research on StSODs and their regulation in potato, particularly in response to elevated temperatures. Full article

This manuscript reveals both the full experimental and methodical details of a most-recent patent that demonstrates a much-desired goal of rotational maneuvers via angular exchange momentum, namely extremely high torque without mathematical singularity and accompanying loss of attitude control while the angular momentum trajectory resides in the mathematical singularity. The paper briefly reviews the most recent literature, and then gives theoretical development for implementing the new control methods described in the patent to compute a non-singular steering command to the angular momentum actuators. The theoretical developments are followed by computer simulations used to verify the theoretical computation methodology, and then laboratory experiments are used for validation on a free-floating hardware simulator. A typical 3/4 CMG array skewed at 54.73° yields 0.15H. Utilizing the proposed singularity penetration techniques, 3H momentum is achieved about yaw, 2H about roll, and 1H about pitch representing performance increases of 1900%, 1233%, and 566% respectfully. Full article

Mechanical loads cause severe damage to perishable agricultural products. In order to quantify the mechanical impact during harvest and postharvest processes, several electronic fruits have been developed. The objective of the work described here was to compare on a laboratory scale different types of impact acceleration recording electronic fruits: Mikras implanted in a real potato tuber as well as in a dummy tuber, IRD, Smart Spud and TuberLog. The acquisition of mechanical impacts was performed using a drop simulator with optional steel or PVC as impact material as well as a processing line simulator. Our results show that drops from 10 cm height on PVC caused similar peak accelerations of Mikras implanted in a real potato or a dummy, IRD and TuberLog. When dropped onto steel however, IRD, TuberLog and Mikras implanted in a dummy recorded higher peak values than Mikras in real potatoes. Impact on the flat side of a tuber led to higher peak values than impact on the apical region. This could be caused by different elastic compliance of synthetic materials as well as material thickness. Running through the processing line simulator TuberLog recorded the most impact; Smart Spud recorded a low number of impacts compared to the other electronic fruits. In all experiments the least sensitive measurements were recorded using Smart Spud. Full article

Real-time quantitative polymerase chain reaction is subject to inhibition by substances that co-purify with nucleic acids during isolation and preparation of samples. Such materials alter the activity of reverse transcriptase (RT) and thermostable DNA polymerase enzymes on which the assay depends. When removal of inhibitory substances by column or reagent-based methods fails or is incomplete, the remaining option of appropriately, precisely and differentially diluting samples and standards to non-inhibitory concentrations is often avoided due to the logistic problem it poses. To address this, we invented the PREXCEL-Q software program to automate the process of calculating the non-inhibitory dilutions for all samples and standards after a preliminary test plate has been performed on an experimental sample mixture. The SPUD assay was used to check for inhibition in each PREXCEL-Q-designed qPCR reaction. When SPUD amplicons or SPUD amplicon-containing plasmids were spiked equally into each qPCR reaction, all reactions demonstrated complete absence of qPCR inhibition. Reactions spiked with ~15,500 SPUD amplicons yielded a Cq of 27.39 +/- 0.28 (at ~80.8% efficiency), while reactions spiked with ~7,750 SPUD plasmids yielded a Cq of 23.82 +/- 0.15 (at ~97.85% efficiency). This work demonstrates that PREXCEL-Q sample and standard dilution calculations ensure avoidance of qPCR inhibition. Full article