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Keywords = Oculatella

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29 pages, 8314 KiB  
Article
Hot Is Rich—An Enormous Diversity of Simple Trichal Cyanobacteria from Yellowstone Hot Springs
by Jan Kaštovský, Jeffrey R. Johansen, Radka Hauerová and Mildred U. Akagha
Diversity 2023, 15(9), 975; https://doi.org/10.3390/d15090975 - 28 Aug 2023
Cited by 17 | Viewed by 7163
Abstract
In 2019, cyanobacterial samples were collected from thermal sites in Yellowstone National Park. In this paper, we discuss the considerable richness of representatives of simple filamentous cyanobacteria. Using a polyphasic approach, one new genus, Copelandiella, with two species (C. yellowstonensis and [...] Read more.
In 2019, cyanobacterial samples were collected from thermal sites in Yellowstone National Park. In this paper, we discuss the considerable richness of representatives of simple filamentous cyanobacteria. Using a polyphasic approach, one new genus, Copelandiella, with two species (C. yellowstonensis and C. thermalis) and eight additional new species Albertania prattii, K. anagnostidisii, Kovacikia brockii, Leptolyngbya tildenae, L. vaporiphila, Nodosilinea calida, N. igneolacustris, and Oculatella castenholzii were described. At the same time, by analyzing our new molecular data, we concluded that other genera of trichal cyanobacteria can be merged with existing genera: species within the genus Plectolyngbya are herein transferred to Leptolyngbya, and if some nomenclatural issues are resolved, species within the genus Leptothermofonsia will be subsequently transferred to the genus Kovacikia, an earlier synonym. Full article
(This article belongs to the Special Issue The Phylogenetic Diversity of Cyanobacteria and Algae)
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13 pages, 3126 KiB  
Article
Identification of Putative Biosynthetic Gene Clusters for Tolyporphins in Multiple Filamentous Cyanobacteria
by Xiaohe Jin, Yunlong Zhang, Ran Zhang, Kathy-Uyen Nguyen, Jonathan S. Lindsey and Eric S. Miller
Life 2021, 11(8), 758; https://doi.org/10.3390/life11080758 - 28 Jul 2021
Cited by 8 | Viewed by 3150
Abstract
Tolyporphins A–R are unusual tetrapyrrole macrocycles produced by the non-axenic filamentous cyanobacterium HT-58-2. A putative biosynthetic gene cluster for biosynthesis of tolyporphins (here termed BGC-1) was previously identified in the genome of HT-58-2. Here, homology searching of BGC-1 in HT-58-2 led to identification [...] Read more.
Tolyporphins A–R are unusual tetrapyrrole macrocycles produced by the non-axenic filamentous cyanobacterium HT-58-2. A putative biosynthetic gene cluster for biosynthesis of tolyporphins (here termed BGC-1) was previously identified in the genome of HT-58-2. Here, homology searching of BGC-1 in HT-58-2 led to identification of similar BGCs in seven other filamentous cyanobacteria, including strains Nostoc sp. 106C, Nostoc sp. RF31YmG, Nostoc sp. FACHB-892, Brasilonema octagenarum UFV-OR1, Brasilonema octagenarum UFV-E1, Brasilonema sennae CENA114 and Oculatella sp. LEGE 06141, suggesting their potential for tolyporphins production. A similar gene cluster (BGC-2) also was identified unexpectedly in HT-58-2. Tolyporphins BGCs were not identified in unicellular cyanobacteria. Phylogenetic analysis based on 16S rRNA and a common component of the BGCs, TolD, points to a close evolutionary history between each strain and their respective tolyporphins BGC. Though identified with putative tolyporphins BGCs, examination of pigments extracted from three cyanobacteria has not revealed the presence of tolyporphins. Overall, the identification of BGCs and potential producers of tolyporphins presents a collection of candidate cyanobacteria for genetic and biochemical analysis pertaining to these unusual tetrapyrrole macrocycles. Full article
(This article belongs to the Special Issue Cyanobacteria: Advances in (Meta-)Genomics and Proteomics)
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