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Keywords = Obesumbacterium proteus

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31 pages, 4000 KiB  
Article
Assessment of Recombinant β-Propeller Phytase of the Bacillus Species Expressed Intracellularly in Yarrowia lipolityca
by Liliya G. Maloshenok, Yulia S. Panina, Sergey A. Bruskin, Victoria V. Zherdeva, Natalya N. Gessler, Alena V. Rozumiy, Egor V. Antonov, Yulia I. Deryabina and Elena P. Isakova
J. Fungi 2025, 11(3), 186; https://doi.org/10.3390/jof11030186 - 26 Feb 2025
Viewed by 668
Abstract
Phytases of the PhyD class according to their pH optimum (7.0–7.8) and high thermal stability can claim to be used in the production of feed supplements. However, today they have no practical application in feed production because there are no suitable producers sufficient [...] Read more.
Phytases of the PhyD class according to their pH optimum (7.0–7.8) and high thermal stability can claim to be used in the production of feed supplements. However, today they have no practical application in feed production because there are no suitable producers sufficient for its biotechnological production compared to the PhyA and PhyC class ones. Moreover, in most cases, the technologies with the enzymes produced in secretory form are preferable for the production of phytases, though upon microencapsulation in yeast-producing cells, the phytase thermal stability increases significantly compared to the extracellular form, which improves its compatibility with spray drying technology. In this study, we assayed the intracellular heterologous expression of PhyD phytase from Bacillus species in the Yarrowia lipolytica yeast cells. While the technology has been successfully used to synthesize PhyC phytase from Obesumbacterium proteus, PhyD phytase tends to aggregate upon intracellular accumulation. Furthermore, we evaluated the prospects for the production of encapsulated phytase of the PhyD class of high enzymatic activity when it accumulates in the cell cytoplasm of the Y. lipolytica extremophile yeast, a highly effective platform for the production of recombinant proteins. Full article
(This article belongs to the Special Issue New Trends in Yeast Metabolic Engineering)
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22 pages, 2844 KiB  
Article
The Efficacy of Encapsulated Phytase Based on Recombinant Yarrowia lipolytica on Quails’ Zootechnic Features and Phosphorus Assimilation
by Ekanerina A. Ovseychik, Olga I. Klein, Natalia N. Gessler, Yulia I. Deryabina, Valery S. Lukashenko and Elena P. Isakova
Vet. Sci. 2024, 11(2), 91; https://doi.org/10.3390/vetsci11020091 - 15 Feb 2024
Cited by 2 | Viewed by 3349
Abstract
In this study, we used the Manchurian golden breed of quails. We assessed the efficacy of the food additives of the phytase from Obesumbacterium proteus encapsulated in the recombinant Yarrowia lipolytica yeast, which was supplied at a concentration of 500 phytase activity units [...] Read more.
In this study, we used the Manchurian golden breed of quails. We assessed the efficacy of the food additives of the phytase from Obesumbacterium proteus encapsulated in the recombinant Yarrowia lipolytica yeast, which was supplied at a concentration of 500 phytase activity units per kg of the feed. One hundred fifty one-day-old quails were distributed into six treatment groups. The results showed that adding the O. proteus encapsulated phytase to the quails’ diets improved live weight, body weight gain, and feed conversion compared to those in the control groups and the groups using a commercial phytase from Aspergillus ficuum. The results obtained during the experiments indicate a high degree of assimilation of phytate-containing feeds if the encapsulated phytase was fed by the quails compared to that in the other groups. We can conclude that the class D encapsulated phytase is an expedient additive to the diets possessing better kinetic features compared to the PhyA and PhyC classes phytases when it acts inside the quail’s chyme. Full article
(This article belongs to the Section Veterinary Food Safety and Zoonosis)
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15 pages, 560 KiB  
Article
Encapsulated Phytase Produced by Recombinant Yarrowia lipolytica Exhibits High Efficiency on Broiler Chickens in Low Dosage
by Maria A. Danilova, Ekaterina Yu. Epova, Elena V. Trubnikova, Niyaz V. Badrutdinov, Anastasya S. Kokoreva, Maxim S. Pusev, Yulia I. Deryabina and Elena P. Isakova
Appl. Sci. 2022, 12(23), 11999; https://doi.org/10.3390/app122311999 - 24 Nov 2022
Cited by 6 | Viewed by 1857
Abstract
Phytases are the largest group of feed enzymes increasing the accessibility of organic phosphorus for the animals. Feed phytases are usually sold as dried powder of secreting producers, mainly micellar fungi. We proposed a new technology for producing phytase from Obesumbacterium proteus (OPP) [...] Read more.
Phytases are the largest group of feed enzymes increasing the accessibility of organic phosphorus for the animals. Feed phytases are usually sold as dried powder of secreting producers, mainly micellar fungi. We proposed a new technology for producing phytase from Obesumbacterium proteus (OPP) in yeast Yarrowia lipolytica as cytosolic protein (encapsulated OPP), where the capsule (yeast cell) protects the enzyme from unfavourable factors (acid medium and active proteolysis in stomachs) and releases it along with the substrate in the duodenum only. Here we report results of testing the encapsulated OPP on the model of a broiler chicken in comparison to a conventional phytase from Aspergillus ficuum. The encapsulated OPP at a dosage of 30 FYT/kg provided the maximum body weight of the chicken in the end of experiment equal or somewhat higher than in the control group, where the available phosphorus deficit was complemented with a mineral phosphorus supply. In contrast, the conventional soluble phytase at a dosage of 100 or 1000 FYT/kg was not able to compensate for the phosphorus deficit in the diet, although chemical analysis demonstrated much phosphorus in the diet in a non-accessible form. The encapsulated OPP decreased the residual Pi in the chicken faeces by 2.1 times in comparison to the control when added to the diet, whereas the conventional phytases negligibly affected this parameter regardless of the dosage. Full article
(This article belongs to the Section Applied Industrial Technologies)
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11 pages, 575 KiB  
Article
A Feed Additive Containing Encapsulated 6-Phytase within Recombinant Yarrowia lipolytica Cells Produced by Cultivation on Fat-Containing Waste
by Maria A. Danilova, Ekaterina Yu. Epova, Elena V. Trubnikova and Alexei B. Shevelev
Appl. Sci. 2022, 12(6), 3094; https://doi.org/10.3390/app12063094 - 17 Mar 2022
Cited by 5 | Viewed by 2309
Abstract
Feed phytases are purchased as a dry culture medium of secreting producers, mostly micellar fungi. These preparations are required to withstand heating up to 75–80 °C because they are intended for mixing with feed components with subsequent granulation by spray drying. For this [...] Read more.
Feed phytases are purchased as a dry culture medium of secreting producers, mostly micellar fungi. These preparations are required to withstand heating up to 75–80 °C because they are intended for mixing with feed components with subsequent granulation by spray drying. For this reason, many phytases that have a high specific activity at 37 °C and correspond to the optimal pH of intestinal chyme are not used in practice. A novel expression system allowing accumulation of the phytase from Obesumbacterium proteus within yeast Yarrowia lipolytica was proposed. Encapsulation increases thermal stability of the enzyme from 55 °C up to 70 °C. The obtained preparation exhibited a high impact on the daily weight gain of a weaned mouse model fed a phosphorus-deficient diet at a dosage 165 phytase activity units (FYT)/kg, whereas a commercial phytase preparation—Ladozyme Proxi derived from Aspergillus ficuum—did not improve the daily weight gain even at the dosage of 15,000 FYT/kg. Full article
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