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Keywords = NCL cell library

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13 pages, 1759 KB  
Article
Impairment of Nucleolin Activity and Phosphorylation by a Trachylobane Diterpene from Psiadia punctulata in Cancer Cells
by Maria Laura Bellone, Lorenzo Fiengo, Carmen Cerchia, Roberta Cotugno, Ammar Bader, Antonio Lavecchia, Nunziatina De Tommasi and Fabrizio Dal Piaz
Int. J. Mol. Sci. 2022, 23(19), 11390; https://doi.org/10.3390/ijms231911390 - 27 Sep 2022
Cited by 4 | Viewed by 4109
Abstract
Human nucleolin (hNcl) is a multifunctional protein involved in the progression of various cancers and plays a key role in other pathologies. Therefore, there is still unsatisfied demand for hNcl modulators. Recently, we demonstrated that the plant ent-kaurane diterpene oridonin inhibits hNcl but, [...] Read more.
Human nucleolin (hNcl) is a multifunctional protein involved in the progression of various cancers and plays a key role in other pathologies. Therefore, there is still unsatisfied demand for hNcl modulators. Recently, we demonstrated that the plant ent-kaurane diterpene oridonin inhibits hNcl but, unfortunately, this compound is quite toxic for healthy cells. Trachylobane diterpene 6,19-dihydroxy-ent-trachiloban-17-oic acid (compound 12) extracted from Psiadia punctulata (DC.) Vatke (Asteraceae) emerged as a ligand of hNcl from a cellular thermal shift assay (CETSA)-based screening of a small library of diterpenes. Effective interaction between this compound and the protein was demonstrated to occur both in vitro and inside two different types of cancer cells. Based on the experimental and computational data, a model of the hNcl/compound 12 complex was built. Because of this binding, hNcl mRNA chaperone activity was significantly reduced, and the level of phosphorylation of the protein was affected. At the biological level, cancer cell incubation with compound 12 produced a cell cycle block in the subG0/G1 phase and induced early apoptosis, whereas no cytotoxicity towards healthy cells was observed. Overall, these results suggested that 6,19-dihydroxy-ent-trachiloban-17-oic could represent a selective antitumoral agent and a promising lead for designing innovative hNcl inhibitors also usable for therapeutic purposes. Full article
(This article belongs to the Special Issue Natural Compounds in Cancer Therapy and Prevention)
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19 pages, 15654 KB  
Article
A Methodology to Design Static NCL Libraries
by Toi Le Thanh, Lac Truong Tri and Trang Hoang
J. Low Power Electron. Appl. 2022, 12(2), 31; https://doi.org/10.3390/jlpea12020031 - 6 Jun 2022
Cited by 1 | Viewed by 3439
Abstract
The Null Convention Logic (NCL) based asynchronous design technique has interested researchers because this technique had overcome disadvantages of the synchronous technique, such as noise, glitches, clock skew and power. However, using the NCL-based asynchronous design method is difficult for university students and [...] Read more.
The Null Convention Logic (NCL) based asynchronous design technique has interested researchers because this technique had overcome disadvantages of the synchronous technique, such as noise, glitches, clock skew and power. However, using the NCL-based asynchronous design method is difficult for university students and researchers because of the lack of standard NCL cell libraries. Therefore, in this paper, a novel flow is proposed to design NCL cell libraries. These libraries are used to synthesize NCL-based asynchronous designs. We chose the static NCL cell library to illustrate the proposed design solution because this library is one of the most basic NCL libraries. Static NCL cells in this library are designed based on the Process Design Kit 45nm technology and are implemented by the Virtuoso and the Design Compiler (DC) tool. In addition, the Ocean script and Electronic Design Automation (EDA) environment are used for supporting designs and simulations. A complete library of 27 NCL cells was designed to serve for study and research. We also implemented synthesis for NCL full adders using this library and compared our synthesis results with the results of other authors. The comparison results indicated that our results were a 20% improvement on power consumption. Full article
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