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This paper illustrates the origin, mission, organization and main activities of the Interdivisional Group of Separation Science (Gruppo Interdivisionale di Scienza delle Separazioni, GISS) of the Italian Chemical Society in occasion of the twentieth anniversary of this organization, whose mission is to promote and disseminate knowledge on all aspects of separation science and to facilitate strong peer-to-peer relationships. GISS originates from a discussion group, also belonging to the Italian Chemical Society (SCI), having similar finality and the same promoter (the Italian pioneer of Chromatography Prof. Arnaldo Liberti), but less extended scientific interests than the GISS, since it was mainly focused on liquid and gas chromatography. The interests of the GISS span theoretical and practical aspects of separation science and cover all key topics requiring high-performance separation techniques to carry out advanced investigations and/or solve challenging analytical problems. The panoramic view of the GISS includes a synthetic review of the main activities carried out since its foundation, which comprises fast-track discussion meetings, participation, promotion and/or organization of national and international congresses, sponsorship of participation grants for young trainees and Ph.D. students, recognition of scientists who have made and continue to make an outstanding contribution to separation science, as well as young scientists who have evidenced their attitude to carry out significant studies in the field of separation science. Full article