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Keywords = INUSpheresis®

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13 pages, 514 KB  
Article
Selective Removal of Plasma Proteins by Double-Filtration Plasmapheresis in Canine Blood: An Ex Vivo Study and Retrospective Report of In Vivo Clinical Treatments in Three Dogs
by Roberta Troia, Claudia Iannucci, Lisa Niemann and Alessio Vigani
Vet. Sci. 2025, 12(6), 528; https://doi.org/10.3390/vetsci12060528 - 29 May 2025
Viewed by 3764
Abstract
Double-filtration plasmapheresis (DFPP) is a plasma-exchange modality that allows selective clearance of high-molecular-weight proteins, potentially minimizing albumin loss and the need for volume replacement. Reports concerning DFPP use in dogs are scarce. This study evaluates the quantitative net loss of different plasma proteins [...] Read more.
Double-filtration plasmapheresis (DFPP) is a plasma-exchange modality that allows selective clearance of high-molecular-weight proteins, potentially minimizing albumin loss and the need for volume replacement. Reports concerning DFPP use in dogs are scarce. This study evaluates the quantitative net loss of different plasma proteins fractions in an ex vivo model using canine blood processed via DFPP. DFPP treatment with INUSpheresis® processing 1.5, 2, and 3 plasma volumes (PVs) was performed. Plasma proteins fractions were measured in the reservoir blood bag at baseline (pre-treatment) and in the effluent bag at the end of each target PV exchanged to calculate the net loss of selected plasma proteins. At 1.5 PV, net globulin and albumin loss was 41 and 25% respectively. At 3 PV, net globulin and albumin loss was 47 and 40%, respectively. Fibrinogen concentration was unmeasurable low in the reservoir blood bag after processing 1.5 PV. INUSpheresis® allows selective plasma proteins removal, with a sparing effect on albumin at 1.5 PV. Selectivity is, however, progressively reduced with incremental target PV. A description of five DFPP treatments in three dogs is additionally presented. Semi-selective protein removal was also demonstrated in vivo, with a significantly lower percentage reduction in albumin compared to total globulin (p = 0.01) and fibrinogen (p = 0.007). Full article
(This article belongs to the Section Veterinary Internal Medicine)
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11 pages, 2668 KB  
Article
Particles in the Eluate from Double Filtration Plasmapheresis—A Case Study Using Field Emission Scanning Electron Microscopy/Energy-Dispersive X-ray Spectroscopy (FE-SEM/EDX)
by Felix Scholkmann and Antonietta M. Gatti
Compounds 2022, 2(4), 367-377; https://doi.org/10.3390/compounds2040030 - 1 Dec 2022
Viewed by 8709
Abstract
Unwanted substances can be effectively removed from the blood using double-filtration plasmapheresis (DFPP). In our case study, we used field emission scanning electron microscopy/energy-dispersive X-ray analysis (FE-SEM-EDX) to examine if the eluate obtained by a specific type of DFPP (INUSpheresis with a TKM58 [...] Read more.
Unwanted substances can be effectively removed from the blood using double-filtration plasmapheresis (DFPP). In our case study, we used field emission scanning electron microscopy/energy-dispersive X-ray analysis (FE-SEM-EDX) to examine if the eluate obtained by a specific type of DFPP (INUSpheresis with a TKM58 filter) contains nano- and microparticles and what chemical composition these particles have. We identified micro- and nanoparticles of various sizes and chemical composition, including microparticles high in the concentration of calcium, iron, silicon, aluminium and titanium. Furthermore, thread-like objects were identified. We discuss the possible origin of the particles and objects, their pathophysiological relevance and the potential of FE-SEM-EDX analysis of the eluate in terms of diagnostics and therapy for environmental medicine applications on patients. Full article
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12 pages, 2631 KB  
Article
Changes in Water Properties in Human Tissue after Double Filtration Plasmapheresis—A Case Study
by Felix Scholkmann and Roumiana Tsenkova
Molecules 2022, 27(12), 3947; https://doi.org/10.3390/molecules27123947 - 20 Jun 2022
Cited by 6 | Viewed by 6125
Abstract
Double-filtration plasmapheresis (DFPP) is a blood cleaning technique that enables the removal of unwanted substances from the blood. In our case study, we performed near-infrared (NIR) spectroscopy measurements on the human hand tissue before and after a specific DFPP treatment (INUSpheresis with a [...] Read more.
Double-filtration plasmapheresis (DFPP) is a blood cleaning technique that enables the removal of unwanted substances from the blood. In our case study, we performed near-infrared (NIR) spectroscopy measurements on the human hand tissue before and after a specific DFPP treatment (INUSpheresis with a TKM58 filter), along with NIR measurements of the substances extracted via DFPP (eluate). The spectral data were analyzed using the aquaphotomics approach. The analysis showed that the water properties in the tissue change after DFPP treatment, i.e., an increase in small water clusters, free water molecules and a decrease in hydroxylated water as well as superoxide in hydration shells was noted. The opposite effect was observed in the eluates of both DFPP treatments. Our study is the first that documents changes in water spectral properties after DFPP treatments in human tissue. The changes in tissue water demonstrated by our case study suggest that the positive physiological effects of DFPP in general, and of INUSpheresis with the TKM58 filter in particular, may be associated with improvements in water quality in blood and tissues. Full article
(This article belongs to the Special Issue Aquaphotomics - Exploring Water Molecular Systems in Nature)
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