Search Results (11)

Background: Campylobacter causes gastroenteritis worldwide with increasing antimicrobial resistance. Furazolidone (FZD) shows potential in resource-poor areas but needs further study. We aimed to assess the in vitro susceptibility of Campylobacter spp. to FZD, ciprofloxacin (CIP), and erythromycin (ERY) in a high-risk pediatric cohort and to evaluate the clinical relevance of resistance patterns using inhibitory quotient (IQ) pharmacodynamics. Methods: A two-phase prospective study (2012–2013, 2014–2015) was conducted at a tertiary pediatric hospital in Lima, Peru. Stool samples from children ≤24 months were cultured on selective media, with Campylobacter isolates identified via conventional bacteriological methods. Antimicrobial susceptibility was determined using Kirby–Bauer disk diffusion and regression-derived minimum inhibitory concentrations (MICs). IQ analysis correlated inhibition zones with therapeutic outcomes. Results: Among 194 Campylobacter isolates (C. jejuni: 28%; C. coli: 72%), resistance to CIP declined from 97.7% (2012–2013) to 83% (2014–2015), while ERY resistance rose from 2.3% to 9.4% (p= 0.002). No FZD resistance was observed, with mean inhibition zones of 52 ± 8 mm (2012–2013) and 43 ± 10.5 mm (2014–2015). MICs for FZD were predominantly <0.125 μg/mL, and all susceptible isolates demonstrated favorable IQ outcomes. Multidrug resistance (≥2 drugs) increased to 6.2% (2014–2015), though all MDR strains retained FZD susceptibility. CLSI and EUCAST breakpoints showed concordance for ERY (p = 0.724) but discordance for CIP (p = 0.022 vs. 0.008). Conclusions: FZD exhibits sustained in vitro efficacy against Campylobacter spp., even among MDR strains, contrasting with escalating fluoroquinolone and macrolide resistance. Full article

Computer-Assisted Language Learning (CALL) is an umbrella term that encompasses diverse technologies with the purpose of enhancing language learning. In the existing literature on CALL, intercultural awareness and the pedagogical use of multimedia products have received less attention. This study explores how the process of creating subtitles for short clips may enhance language skills and intercultural awareness when implemented through lesson plans designed following the framework proposed by the TRADILEX project. A pre-experimental longitudinal design was implemented. The sample consisted of 43 participants who were enrolled in a B2 English course at the University of Córdoba (Spain). During the course, participants consistently attended theoretical sessions. The intervention took place during the practical sessions from February to April 2024, involving four subtitling-based lesson plans on literature and gender. After the intervention, the practical sessions shifted to a traditional, textbook-based format from April to June 2024. The instruments employed to assess the effectiveness of the intervention consisted of a commercial test by MacMillan and the ERI scale on interculturality. The results showed that after the intervention, there was a significant improvement in language proficiency, which increased at a slower rate during the traditional sessions. However, when it comes to intercultural awareness, there was a peak of attainment after the intervention, but attrition rapidly took place. Regarding the pedagogical implications of this study, subtitling could be an appropriate technique that allows contact with the L2 culture and shows positive effects in terms of proficiency. Full article

Tetanus neurotoxins (TeNT) and botulinum neurotoxins (BoNTs) are closely related ~150 kDa protein toxins that together comprise the group of clostridial neurotoxins (CNTs) expressed by various species of Clostridia. While TeNT is expressed as a single polypeptide, BoNTs are always produced alongside multiple non-toxic proteins that form a stabilizing complex with BoNT and are encoded in a conserved toxin gene cluster. It is unknown how tent evolved without a similar gene cluster and why complex-free TeNT is secreted as a stable and soluble protein by C. tetani, whereas complexing proteins appear to be essential for BoNT stability in culture supernatants of C. botulinum. To assess whether the stability of TeNT is due to an innate property of the toxin or is a result of C. tetani’s intra- and extra-cellular environment, both TeNT and complex-free BoNT/A1^ERY were expressed recombinantly in atoxic C. tetani and analyzed for expression and stability. The strong clostridial ferredoxin (fdx) promotor resulted in the expression of recombinant TeNT at greater levels and earlier time points than endogenously produced TeNT. Recombinant BoNT/A1^ERY was similarly expressed by atoxic C. tetani, although partial degradation was observed. The rBoNT/A1^ERY produced in C. tetani was also partially proteolytically processed to the dichain form. Investigations of bacterial growth media and pH conditions found that the stability of rTeNT and rBoNT/A1^ERY in spent media of C. tetani or C. botulinum was affected by growth media but not by pH. These data indicate that the distinct metabolism of C. tetani or C. botulinum under various growth conditions is a primary factor in creating a more or less favorable environment for complex-free CNT stability. Full article

The newly constructed Bois d’Arc Lake Reservoir in Fannin County, Texas, USA, inevitably flooded a large ground surface area (67.34 km²) when the reservoir began impounding water in April 2021. Inside this (now) flooded area, land-based archaeological data recovery investigations discovered and documented several archaeological sites, now registered in the state of Texas; though, only two neighboring sites, namely, 41FN178 and 41FN244, are examined here. The first phase of archaeological testing at these sites included shovel testing, test unit excavations, and geoarchaeological trenching that yielded archaeological artifacts suggesting that Middle Caddo Indian peoples (AD 1200–1400) might have occupied this landscape. As the sites were recognized before the reservoir’s impoundment phase, this merited a non-invasive, non-destructive, high-resolution near-surface geophysical study to map strategic areas within sites 41FN178 and 41FN244 that might yield potential shallow targets of archaeological context. The adopted geophysical survey comprised 3D direct current electrical resistivity imaging (ERI) and land horizontal magnetic gradiometry (HMG), each mapping a total surface area of 2133 and 15,640 m², respectively. The combination of 3D ERI and land HMG surveys was instrumental in rapidly mapping the horizontal and vertical extent of shallowly buried anomalies within a large area prior to the completion of the dam and the beginning of water impoundment. Based on the geophysical insights, the outline of several Caddo houses with functional internal and external features (e.g., burnt cooking surfaces, storage pits, refuse pits, fired soil, ditches, a dump site, and a compound fence) are thought to exist within the uppermost 2 m of the Quaternary stratigraphy at both sites. At site 41FN244, 3D ERI found numerous resistive anomalies surrounding a conductive anomaly, collectively interpreted as a group of post-holes surrounding the remains of a Caddo house’s inner clay floor. It also found a cluster of several resistive anomalies interpreted as midden or middens. The HMG survey carried across areas from which archaeological test units also yielded positive findings, at sites 41FN178 and 41FN244, identified numerous scattered monopolar and dipolar anomalies interpreted as post-molds of Caddo houses, compound enclosures or fences, and adjacent middens. Archaeological excavations guided by the geophysical results yielded significant cultural material and post-mold features at site 244, which validate the geophysical interpretation in a preliminary context. Additionally, several dispersed magnetic anomalies are thought to be shallowly buried hearths, burn cooking surfaces, storage pits, and ditches. The mapped magnetic anomalies agree with the location and distribution of previously found archaeological artifacts and the extent of resistive and conductive resistivity anomalies. Follow-up archaeological excavations of these geophysical anomalies have preliminarily confirmed interpretations. Full article

Campylobacter spp. are significant zoonotic agents, which cause annually millions of human cases of foodborne gastroenteritis worldwide. Their inclusion in biofilms on abiotic surfaces seems to play a pivotal role in their survival outside of the host, growth, and spread. To successfully mitigate the risks that arise with these bacteria, it is crucial to decrease their prevalence within the food production chain (from farm to the table), alongside the successful treatment of the resulting illness, known as campylobacteriosis. For this, the use of various antimicrobial agents remains actively in the foreground. A general-purpose biocide and cationic surfactant (benzalkonium chloride; BAC), a widely used macrolide antibiotic (erythromycin; ERY), and a naturally occurring organic acid (L(+)-lactic acid; LA) were comparatively evaluated in this work for their potential to inhibit both the planktonic and biofilm growth of 12 selected Campylobacter spp. (of which, seven were C. jejuni and five were C. coli) raw chicken meat isolates, all grown in vitro as monocultures. The inhibitory action of LA was also studied against four mixed-culture Campylobacter biofilms (each composed of three different isolates). The results showed that the individual effectiveness of the agents varied significantly depending on the isolate, growth mode (planktonic, biofilm), intercellular interactions (monocultures, mixed cultures), and the growth medium used (with special focus on blood presence). Thus, BAC exhibited minimum inhibitory concentrations (MICs), minimum bactericidal concentrations (MBCs), and minimum biofilm inhibitory concentrations (MBICs) that ranged from 0.5 to 16 μg/mL. Interestingly enough, these values varied widely from 0.25 to 1024 μg/mL for ERY. Concerning LA, the MICs, MBCs, and MBICs varied from 1024 to 4096 μg/mL, with mixed-culture biofilm formation always being more difficult to suppress when compared to biofilm monocultures. In addition, it was evident that intercellular interactions encountered within mixed-culture Campylobacter biofilms significantly influenced both the population dynamics and the tolerance of each consortium member to acid exposure. Overall, the findings of this study provide useful information on the comparative effectiveness of three well-known antimicrobial agents for the control of Campylobacter spp. under various growth modes (i.e., planktonic, biofilm, monocultures, mixed cultures) that could potentially be encountered in food production and clinical settings. Full article

Dimapur (Nagaland, India) is dominated by undifferentiated hillside slopes and alluvial plains. The Muga and Eri silk industries are important cultural and economic activities for the inhabitants of Dimapur. Profitable silk production requires adequate quality and quantity of healthy leaves and is highly dependent on the soil fertility of the region. Keeping this in view, the present study was carried out as a first attempt to prepare a geographic information system (GIS) map for Muga and Eri soils in Dimapur, Nagaland. A total 65 surface (0–15 cm) soil samples from Muga farms and 79 surface soil samples from Eri farms were collected and analysed for soil pH, organic carbon content and availability of macro- and micronutrients. Soils of both Muga and Eri farms were found to be extremely (<3.05) to moderately (5.09–6.84) acidic. Soils of Muga and Eri farms were found to have low to high organic carbon content (from 0.24 to 1.98%), low to high available nitrogen content (179.8–612.5 kg ha⁻¹) and medium available phosphorus content (2.68–154.6 kg ha⁻¹). The sulphur availability index was 0.26 and 11.81 for Muga and Eri host plant farms, respectively. The multi-macronutrient map revealed that 46.95% of the district’s total geographical area (TGA) showed deficiencies in one or more macronutrients (high priority zone). Thus, these regions need urgent attention in terms of nutrient management decisions in order to reduce the declining trend of soil fertility and achieve sustainable sericulture production. Full article

The contribution of biodiversity to human well-being is key to exploring the relationships between biodiversity, ecosystem services (ES), and human well-being. In this work, a composite index, termed the human well-being index (HWI), was constructed for evaluating the contribution of biodiversity to human well-being in the Sanjiangyuan area. This index consists of material, ecological regulation, and spiritual and cultural contributions, represented by the material index (MI), the ecological regulation index (ERI), and the spiritual and cultural index (SCI), respectively. The system was further used to evaluate the spatiotemporal distribution of human well-being at the county level in 2000, 2010, and 2020. HWI increased steadily across Sanjiangyuan over the study period, especially in the western and northeastern counties; its center of gravity shifted in the northward direction. The MI increased (decreased) in the west and northeast (southeast); its center of gravity shifted in the northeast direction. All counties showing changes in the ERI were located in the eastern part of Sanjiangyuan. The center of gravity of ERI did not change significantly. The SCI increased steadily across the study area, but was high in the west and low in the east; the center of gravity shifted in the northwest direction. The study findings can contribute toward quantifying biodiversity contributions to human well-being and the formulation of biodiversity conservation policies. Full article

The paper explores the potential for adaptive mitigation at the Montezuma Wildlife Refuge, Seneca Falls, NY, USA, and surrounds, focusing on landscapes along the historic Erie Canal that inadvertently lowered the water table and shrunk adjacent wetlands. Now the Erie Canal National Heritage Corridor (ECNHC), Montezuma, NY, USA, the canal, and surrounds lack a clear identity but have the potential to be repurposed as green infrastructure to support climate mitigation through the application of natural climate solutions, namely reforestation. Reforestation has been shown to produce the highest potential performance for carbon sequestration, along with a multitude of co-benefits. However, most studies assessing capacity for climate mitigation using nature-based approaches operate at a high level via remote data and do not test hypotheses at smaller scales that require groundtruthing data, parcel-by parcel approaches, and an understanding of landowner values. The initial research question is: can landscape architecture design research contribute to a higher performance of secondary forests and non-productive farmland (fallow lands) for carbon sequestration, while at the same time activating economic territories and improving their landscape qualities? Comparative cartographies are developed to assess secondary forests, including past and future projections. Fallow lands are examined through mapping at various scales, fieldwork, and informal interviews. We find that farmland along the canal has been abandoned over time due to conditions that make farming difficult, such as periodic flooding, ponding of water due to poorly drained soils, and steep drumlin slopes. These same conditions have contributed to a landscape armature—an assemblage of landscapes including the old canal, barge canal and associated heritage landscapes, abandoned farmlands, and existing forests and wetlands. Secondary forests already existing in the area are high performing in relation to carbon sequestration but may lack climate resilience due to threats such as the emerald ash borer (EAB). Design intervention can help support enhanced sequestration, resilience, and adaptation by introducing unique tree plantings in the form of groves and hedgerows. Sustainability is approached by integrating quantifiable performances of secondary forests with projections of spatial, ecological, and cultural values and the continuing monitoring and management of forests over time. The aim is to build a method to test these lands with designs for tree plantings that reveal their potential for increased carbon sequestration, habitat connectivity, and enriched landscape identity. Full article

A total of 51 pentose utilizing lactic acid bacteria (LAB) were isolated from acid-forming bacteria in the midgut of healthy mature Eri silkworm using de Man, Rogosa and Sharpe (MRS) agar containing 10 g/L xylose (MRS-xylose) as the carbon source supplemented with 0.04% (w/v) bromocresol purple. Further analysis of 16S rRNA gene sequences revealed the highest prevalence of up to 35 enterococci isolates, which included 20 isolates of Enterococcus mundtii, followed by Entercoccus faecalis (eight isolates), Weissella cibaria (four isolates), Enterococcus hirae (two isolates), Enterococcus lactis (one isolate), and Enterococcus faecium (one isolate). All 51 LAB isolates showed positive growth on MRS containing a range of polysaccharides as the sole carbon source. All isolates were able to grow and form clear zones on MRS supplemented with 1 g/L xylose, while E. faecalis SC1, E. faecalis SCT2, and E. hirae SX2 showed tannin tolerance ability up to 5 g/L. Moreover, five isolates showed antimicrobial activity against Eri silkworm pathogens, including Bacillus cereus, Staphylococcus aureus, and Proteus vulgaris, with E. hirae SX2 having the highest inhibitory effect. Supplementation of live E. hirae SX2 on castor leaves significantly improved the weight and reduced the silkworm mortality when compared with the control group (p < 0.05). This cocci LAB can be considered as the new probiotic for Eri culture. Additionally, this finding presented the perspective of non-mulberry silkworm that could also be used as the model for further applying to new trends of the sericulture industry. Full article

Among 39 pentose-utilizing lactic acid bacteria (LAB) selected from acid-forming bacteria from the midgut of Eri silkworm, the isolate WX1 was selected with the highest capability to produce optically pure l-lactic acid (l-LA) from glucose, xylose and arabinose with furfural-tolerant properties. The isolate WX1 was identified as Enterococcus mundtii based on 16S rDNA sequence analysis. The conversion yields of l-LA from glucose and xylose by E. mundtii WX1 were 0.97 and 0.68 g/g substrate, respectively. Furthermore, l-LA production by E. mundtii WX1 in various glucose-xylose mixtures indicated glucose repression effect on xylose consumption. The coculture of E. mundtii WX1 and Lactobacillus rhamnosus SCJ9, a homofermentative LAB capable of producing l-LA from glucose clearly showed an improvement of l-LA production from 30 g/L total glucose-xylose (6:4). The results from Plackett–Burman design (PBD) indicated that Tween 80, MnSO₄ and yeast extract (YE) were three medium components that significantly influenced (p < 0.05) l-LA production using the coculture strategy in the presence of 2 g/L furfural. Optimal concentrations of these variables revealed by central composite design (CCD) and response surface methodology (RSM) were 20.61 g/L YE, 1.44 g/L Tween 80 and 1.27 g/L MnSO₄. Based on the optimized medium with 30 g/L total glucose-xylose (6:4), the maximum experimental l-LA value of 23.59 g/L reflecting 0.76 g/g substrate were achieved from 48 h fermentation at 37 °C. l-LA produced by coculture cultivated under standard MRS medium and new optimized conditions were 1.28 and 1.53 times higher than that obtained from single culture by E. mundtii WX1, respectively. This study provides the foundations for practical applications of coculture in bioconversion of lignocellulose particularly glucose-xylose-rich corn stover to l-LA. Full article

Faced with growing stakeholder attention to climate change-related societal impacts, Environmental Research Infrastructures (ERIs) find it difficult to engage beyond their initial user base, which calls for an overarching governance scheme and transnational synergies. Forced by the enormity of tackling climate change, ERIs are indeed broaching collaborative venues, based on the assumption that no given institution can carry out this agenda alone. While strategic, this requires that ERIs address the complexities and barriers towards aligning multiple organizations, national resources and programmatic cultures, including science. Full article