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Search Results (4)

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Keywords = Dalbergia spp.

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14 pages, 1640 KiB  
Article
Study on the Extraction and Identification of DNA from Ten Dalbergia Species
by Changtao Gan, Haishan He and Jian Qiu
Forests 2023, 14(12), 2318; https://doi.org/10.3390/f14122318 - 26 Nov 2023
Viewed by 1704
Abstract
Most Dalbergia species are economically valuable and have been over-exploited, which has raised concerns. The regulation and protection of this genus require accurate and rapid authentication and identification processes. To address the issue of high residual inhibitors in extracted DNA from the Dalbergia [...] Read more.
Most Dalbergia species are economically valuable and have been over-exploited, which has raised concerns. The regulation and protection of this genus require accurate and rapid authentication and identification processes. To address the issue of high residual inhibitors in extracted DNA from the Dalbergia xylem, an optimized DNA extraction experiment was performed on 10 species of Dalbergia wood stored for 1–5 years; in particular, no gene sequence for D. tsoi can be found in the NCBI database. Additionally, universal primers ITS2 were used for PCR amplification and sequencing to confirm the effectiveness of DNA extraction. The results revealed that rinsing the wood with 0.25 M ammonium acetate buffer produced DNA with a high purity, without a significant decrease in the DNA yield. To achieve an optimal DNA yield, the wood DNA should be rinsed with ammonium acetate fewer than three times. All the wood DNA obtained using the kit method and treated with the ammonium acetate buffer rinsing solution one to four times was successfully amplified. The NJ phylogenetic tree constructed based on ITS2 can distinguish D. tsoi from other Dalbergia spp., and the predicted ITS2 secondary structure showed the difference between species. This experiment extracted high-quality DNA from wood, without the need for purification kits, thereby improving the efficiency of the extraction process. The extracted DNA was directly used for follow-up molecular experiments. Full article
(This article belongs to the Special Issue DNA Barcoding for Species Identification of Forest Organisms)
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23 pages, 1116 KiB  
Review
Baccharis dracunculifolia and Dalbergia ecastophyllum, Main Plant Sources for Bioactive Properties in Green and Red Brazilian Propolis
by Adela Ramona Moise and Otilia Bobiş
Plants 2020, 9(11), 1619; https://doi.org/10.3390/plants9111619 - 21 Nov 2020
Cited by 52 | Viewed by 6368
Abstract
Nowadays, propolis is used as a highly valuable product in alternative medicine for improving health or treating a large spectrum of pathologies, an ingredient in pharmaceutical products, and also as a food additive. Different vegetal materials are collected by honeybees and mixed with [...] Read more.
Nowadays, propolis is used as a highly valuable product in alternative medicine for improving health or treating a large spectrum of pathologies, an ingredient in pharmaceutical products, and also as a food additive. Different vegetal materials are collected by honeybees and mixed with wax and other own substances in order to obtain the final product, called propolis. It is known as the bee product with the widest chemical composition due to the raw material collected by the bees. Different types are known worldwide: green Brazilian propolis (having Baccharis dracunculifolia as the major plant source), red Brazilian propolis (from Dalbergia ecastophyllum), European propolis (Populus nigra L.), Russian propolis (Betula verrucosa Ehrh), Cuban and Venezuelan red propolis (Clusia spp.), etc. An impressive number of scientific papers already demonstrate the pharmacological potential of different types of propolis, the most important activities being the antimicrobial, anti-inflammatory, antitumor, immunomodulatory, and antioxidant activities. However, the bioactive compounds responsible for each activity have not been fully elucidated. This review aims to collect important data about the chemical composition and bioactive properties of the vegetal sources and to compare with the chemical composition of respective propolis types, in order to determine the connection between the floral source and the propolis properties. Full article
(This article belongs to the Special Issue Plants: Sources of Diversity in Propolis Properties)
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20 pages, 2419 KiB  
Review
Recent Advances in Synthesis, Bioactivity, and Pharmacokinetics of Pterostilbene, an Important Analog of Resveratrol
by Yeju Liu, Yuyang You, Juan Lu, Xi Chen and Zhihong Yang
Molecules 2020, 25(21), 5166; https://doi.org/10.3390/molecules25215166 - 6 Nov 2020
Cited by 82 | Viewed by 8713
Abstract
Pterostilbene is a natural 3,5-dimethoxy analog of resveratrol. This stilbene compound has a strong bioactivity and exists widely in Dalbergia and Vaccinium spp. Besides natural extraction, pterostilbene can be obtained by biosynthesis. Pterostilbene has become popular because of its remarkable pharmacological activities, such [...] Read more.
Pterostilbene is a natural 3,5-dimethoxy analog of resveratrol. This stilbene compound has a strong bioactivity and exists widely in Dalbergia and Vaccinium spp. Besides natural extraction, pterostilbene can be obtained by biosynthesis. Pterostilbene has become popular because of its remarkable pharmacological activities, such as anti-tumor, anti-oxidation, anti-inflammation, and neuroprotection. Pterostilbene can be rapidly absorbed and is widely distributed in tissues, but it does not seriously accumulate in the body. Pterostilbene can easily pass through the blood-brain barrier because of its low molecular weight and good liposolubility. In this review, the studies performed in the last three years on resources, synthesis, bioactivity, and pharmacokinetics of pterostilbene are summarized. This review focuses on the effects of pterostilbene on certain diseases to explore its targets, explain the possible mechanism, and look for potential therapeutic applications. Full article
(This article belongs to the Section Natural Products Chemistry)
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11 pages, 3359 KiB  
Article
Identification of Three Dalbergia Species Based on Differences in Extractive Components
by Xiaoqian Yin, Anmin Huang, Shifeng Zhang, Ru Liu and Fang Ma
Molecules 2018, 23(9), 2163; https://doi.org/10.3390/molecules23092163 - 28 Aug 2018
Cited by 39 | Viewed by 5502
Abstract
Dalbergia cultrate, Dalbergia latifolia, and Dalbergia melanoxylon are precious and valuable traded timber species of the genus Dalbergia. For chemotaxonomical discrimination between these easily confused species, the total extractive content of the three wood species was determined using four different [...] Read more.
Dalbergia cultrate, Dalbergia latifolia, and Dalbergia melanoxylon are precious and valuable traded timber species of the genus Dalbergia. For chemotaxonomical discrimination between these easily confused species, the total extractive content of the three wood species was determined using four different organic solvents. Fourier transform infrared (FTIR) spectroscopy was used to analyze functional group differences in the extractive components, inferring the types of principal chemical components according to characteristic peak positions, intensities, and shapes. Gas chromatography-mass spectrometry (GC-MS) was carried out a detailed characterization of the extractive components. The relative content of individual chemical components was determined by area normalization. Results revealed differences in the chemical components and total and individual extract contents of the three Dalbergia species, indicating that FTIR and GC-MS spectroscopy can be applied to identify and discriminate between Dalbergia cultrate, Dalbergia latifolia, and Dalbergia melanoxylon. Full article
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