Search Results (48)

The impact of counterion structure, especially variations in constitutional and stereochemical isomers, on the properties and performance of AABSs remains under-explored. This study investigates how structural variations, particularly the stereochemistry of diammonium cyclohexane (DACH) counterions, influence the self-assembly behavior of AABSs. Four AABSs: undecanoyl-glycine, -L-alanine, -L-valine, and -L-leucine, were paired with six DACH counterions representing cis/trans isomers of 1,2-, 1,3-, and 1,4-DACH. Critical micelle concentrations (CMCs) were determined via conductimetry, and micellar sizes were measured using dynamic light scattering. The degree of counterion binding (β) was calculated to probe micelle stability, while geometry-optimized structures of the DACH isomers were obtained using density functional theory. Lastly, pH measurements were taken to probe the protonation of DACH counterions at their natural pH, where both the DACH counterion and AABS headgroups intrinsically behave as buffers. Results indicate that while surfactant hydrophobicity primarily dictates CMC in other AABS/DACH combinations, trans-1,3-DACH leads to consistently higher CMCs. This deviation likely arises from its structural conformation, which positions the amine groups an intermediate distance of ~4.4–4.5 Å apart, allowing a small fraction of divalently charged counterions to form strong electrostatic bridging pockets at the micelle interface. These interactions dominate over headgroup effects, leading to elevated and surfactant-independent CMC values. Regarding size and other unusual trends in the systems, cis- isomers formed slightly larger micelles, and trans-1,4-DACH induces abnormal aggregation in undecanoyl-glycine leading to temperature dependent gel formation. These findings highlight the significant influence of counterion structure on AABS behavior and support counterion design as a strategy for enhancing surfactant performance in sustainable applications. Full article

Menopausal symptoms can substantially impair women’s quality of life, yet large-scale, population-based data from German-speaking regions are lacking. We used data of a cross-sectional online survey among 26,338 women in Germany, Austria, and Switzerland, using validated instruments such as the Menopause Rating Scale (MRS II), Insomnia Severity Index (ISI), and Alcohol Use Disorders Identification Test (AUDIT-C). Additional questions covered weight change, employment, nutrition, and physical activity. We investigated correlations and group differences using descriptive statistics and univariate tests. The average MRS score was 16.94, with 51% classified as severe discomfort and 15% as requiring treatment. Insomnia was common, with 90.3% reporting at least some degree of sleep disturbance and nearly half (48%) meeting criteria for clinical insomnia. Sleep problems were significantly associated with menopausal symptom severity. No correlations were found between MRS scores and reported nutrition and exercise. Unemployment and weight gain were significantly associated with higher symptom burdens. These findings highlight the considerable health burden faced by menopausal women in the DACH region and suggest a substantial unmet need for clinical and public health interventions. Full article

This study aims to investigate the effect of the Pd(II) complex on HeLa cells using computational biology and proteomic analysis. [Pd(dach)Cl₂]-treated HeLa cells were subjected to comparative proteomics analysis using label-free data-independent liquid chromatography-tandem mass spectrometry (LC-MS/MS). In parallel, the informational spectrum method (ISM) was used to predict potential protein interactors of the [Pd(dach)Cl₂] complex in HeLa cells. Proteomics analysis revealed 121 differentially abundant proteins (DAPs). Enrichment analysis of Gene Ontology (GO) annotations revealed ATP hydrolysis and RNA/protein binding as the top molecular functions and RNA splicing and protein–RNA complex organization as the top biological processes. Enrichment analysis of altered canonical pathways pointed out spliceosome and ribosome pathways. The top hub proteins with potential regulatory importance encompassed ribosomal proteins, translational and transcriptional factors, and components of the ribosome assembly machinery. ISM and cross-spectral analysis identified the nucleoplasm and sensor of the single-stranded DNA (SOSS DNA) complex. Proteome analysis showed that [Pd(dach)Cl₂] targets proteins involved in ribosomal biogenesis and RNA splicing, whereas theoretical prediction implies also potential effect on p53 signaling pathway, and thus, alterations of the expression of regulatory proteins involved in cell survival and proliferation. These findings underscore the potential of Pd(II) complexes as anti-cancer agents, warranting further exploration and detailed functional validation. Full article

The rapid expansion of the cosmetic products market, growing consumer eco-consciousness, and stricter packaging regulations, such as the PPWR, present significant challenges for the cosmetic industry. To assess the sustainability of cosmetic packaging, a benchmarking study was conducted across various product categories available in the DACH region (Germany, Austria, and Switzerland) using a set of selected indicators. The findings highlight an urgent need for action to ensure compliance with future PPWR requirements. While glass and aluminum packaging demonstrated high recyclability, plastic tubes often failed to meet the 70% recyclability target due to incompatible material combinations. Key barriers to recyclability included material incompatibility, metallization, and excessive colorization. Additionally, the use of recycled content in plastic and paper-based packaging was generally low, with only a few samples containing secondary materials. Other critical issues included packaging efficiency, the widespread use of secondary packaging, and use of uncertified renewable materials. Addressing these challenges will require industry-wide efforts to enhance material compatibility, increase recycled content, and optimize packaging design for greater sustainability. Full article

The adoption of the PPWR by the Council of the European Union (EU/2025/40) in 2025 intensifies the regulatory pressure on packaging manufacturers and food producers. This necessitates their adaptation of packaging to the new standards and selection of materials by various sustainability criteria and minimum standards. The legal text places particular emphasis on recyclability and the carbon footprint. The dairy industry holds significant economic importance in the DACH region (Germany, Austria, and Switzerland); this study therefore analyzed and compared the recyclability and carbon footprint of selected product categories and diverse packaging options for butter, yogurt, spreads, curd cheese, and cottage cheese. This study found large differences within the product categories, and also between the assessed countries, due to differing waste management systems (collection, sorting, and recycling) and waste streams. A key finding is the substantial discrepancy in glass packaging, which significantly exceeds the emission values of other packaging systems. In terms of recyclability, glass packaging performs well due to the effectiveness of the recycling systems regarding the future effective PPWR. However, significant variations in recyclability were observed between Germany and Austria for tray and cup materials made of PP and PS. Notably, there is a preference for packaging systems without a cardboard sleeve over those with one. This study provides critical insights into the environmental performance of packaging materials in the dairy sector. It highlights the challenges posed by regulatory changes and the urgent need for region-specific strategies. By identifying key areas for improvement in packaging design and waste management, this work lays the foundation for achieving compliance with the PPWR and advancing the transition toward a circular economy in the DACH region. Full article

This study introduces a new method for nowcasting UV Index maps developed within the framework of the Austrian Solar UV Measurement Network. While we focus on the DACH region (Germany, Austria, and Switzerland) in this study, the same methods are routinely applied to nowcast UV Index maps for Europe. The primary objective is to improve public health measures by providing timely and area-wide UV Index values. The UV Index maps are based on clear-sky calculations using data from the Copernicus Atmosphere Monitoring Service. Cloud effects are integrated using cloud modification factors determined from Meteosat Second Generation satellite imagery. To assess the representativeness of the calculated UV Index maps, the corresponding pixel values are compared to ground-based measurements for the year 2022 at 27 locations in the DACH region. For all sky conditions, the satellite-derived UV Index values are within ±1.0 UV Index of the ground-measured UV Index for at least 91% of the data at stations below 500 m a.s.l. and in flatter landscapes. For high-altitude sites and in more pronounced topographies, the values for U1.0 decrease, with the lowest agreement of 74.8% found for the Sonnblick station located at 3109 m a.s.l. Discrepancies arise due to differences in the measurement methods: ground-based measurements capture the local conditions, while satellite-derived values represent the average values over larger areas. The clear-sky deviations are most pronounced at high-altitude, snow-covered sites due to uncertainties in the surface albedo. Under all sky conditions, cloud variability adds further uncertainties, particularly in complex terrain or broken cloud cover scenarios, where satellite cloud data lack the resolution to capture local fluctuations. This study discusses these uncertainties while also highlighting the potential of the generated UV Index maps to provide area-wide information to the population as a valuable complement to ground-based measurements. Full article

Isoindigo (IID)-based non-fullerene acceptors, known for their broad absorption spectra and high charge carrier mobilities, play a crucial role in organic photovoltaics. In this study, two A−DA’D−A type unfused ring acceptors (URAs), IDC8CP-IC and IDC6CP-IC, were designed and synthesized using cyclopentadithiophene (CPDT) and IID core units, each functionalized with different alkyl chains (2-hexyldecyl and 2-octyldodecyl), through an atom- and step-efficient direct C–H arylation (DACH) method. Both URAs, despite the absence of non-covalent conformation locking between CPDT and IID, demonstrated favorable molecular planarity, broad absorption ranges, low band gaps, and high molar absorption coefficients. Notably, IDC6CP-IC exhibited stronger intermolecular charge transfer and J-aggregation. An organic solar cell (OSC) device based on IDC6CP-IC achieved a power conversion efficiency (PCE) of 3.10%, with a broad photoresponse range extending from 400 to 900 nm. This study highlights the significant impact of alkyl chain engineering on material synthesis, photoelectric properties, and corresponding device performance. Furthermore, DACH is shown to be a promising approach for synthesizing IID-based URAs with near-infrared (NIR) absorption, making it an excellent candidate for bulk heterojunction (BHJ) OSC applications. Full article

Transition metal complexes have been used in medicine for several decades, but their intracellular effects are not yet fully elucidated. Therefore, in this study, we investigate biomolecular changes induced by a palladium(II) complex in cervical carcinoma (HeLa) cells as a model to study the subtle changes caused by transition metal ions ingested by the cells. The impact of dichloro(1,2-diaminocyclohexane)palladium(II), [Pd(dach)Cl₂], was studied by synchrotron radiation-based Fourier transform infrared (SR FTIR) spectroscopy, a powerful tool for studying alterations in cellular components’ biochemical composition and biomolecular secondary structure on a single-cell level. A spectral analysis, complemented by statistics, revealed that the Pd(II) complex considerably affected all major types of macromolecules in HeLa cells and induced structural changes in proteins through an increased formation of cross-β-sheets and causes structural rearrangement in deoxyribonucleic acid (DNA) through potential chromosome fragmentation. Although a certain level of lipid peroxidation was detectable by SR FTIR spectroscopy and confirmed by an analysis of cellular lipids by matrix-assisted laser desorption and ionisation time-of-flight mass spectrometry, the oxidative stress is not a significant mechanism by which Pd(II) expresses the effect on the HeLa cells. Full article

The European Union aims for climate neutrality by 2050 and has proposed the Packaging and Packing Waste Regulation (PPWR) to promote a circular economy, focusing on reducing packaging waste. In this context, a comprehensive sustainability assessment for liquid dairy product packaging, including beverage cartons, bottles and to-go cups, in the DACH region (Germany, Austria and Switzerland) was conducted. The aim was to consider various ecological aspects of environmental impacts and circularity. As the aspect of recyclability is a core aspect in the PPWR, the calculation was of central interest in this project. Here, major differences in the waste management infrastructure between countries could be identified. The majority of assessed packaging falls below the PPWR’s 70% recyclability requirement, with Switzerland showing even lower recyclability due to poor packaging collection and recycling infrastructure. Significant discrepancies in packaging efficiency exist, indicating unnecessary resource consumption, especially in the case of to-go cups. Additionally, the carbon footprint of packaging materials can vary up to ten times within certain product categories, negatively impacting the environment. Good results were identified for the use of certified renewable resources. Overall, the results of the assessment demonstrate several areas for improvement in light of forthcoming regulatory requirements, which must be met in Germany and Austria. Full article

Considerable evidence links the “Big Five” personality traits (neuroticism, extroversion, conscientiousness, agreeableness, and openness) with depression. However, potential mediating and moderating factors are less well understood. We utilized data from a cross-sectional survey of 3065 German-speaking adults from the D-A-CH region to estimate multivariable-adjusted odds ratios and 95% confidence intervalsbetween personality traits and lifetime prevalence of depression (overall and stratified by sex and age). We further explored proportions mediated by psychosocial factors optimism, empathy, perspective-taking, work–life balance, and interpersonal trust. High levels of neuroticism were associated with more than two-fold higher odds of depression, whereas higher levels of conscientiousness were associated with approximately 30% lower odds of depression. The association with neuroticism persisted in all investigated subgroups; apparently, stronger associations for females and participants aged ≥60 years did not correspond to statistically significant interactions. Overall and across all strata, the association of neuroticism with depression appeared to be mediated in part by the considered psychosocial factors; optimism explained the largest proportion of the association. Our results provide empirical evidence for the dynamic predisposition model. Further investigations of these relationships are warranted in longitudinal data with more precise outcome assessments. Full article

It is generally accepted that adjacent guanine residues in DNA are the primary target for platinum antitumor drugs and that differences in the conformations of the Pt-DNA adducts can play a role in their antitumor activity. In this study, we investigated the effect of the carrier ligand cis-1,3-diaminocyclohexane (cis-1,3-DACH) upon formation, stability, and stereochemistry of the (cis-1,3-DACH)PtG₂ and (cis-1,3-DACH)Pt(d(GpG)) adducts (G = 9-EthlyGuanine, guanosine, 5′- and 3′-guanosine monophosphate; d(GpG) = deoxyguanosil(3′-5′)deoxyguanosine). A peculiar feature of the cis-1,3-DACH carrier ligand is the steric bulk of the diamine, which is asymmetric with respect to the Pt-coordination plane. The (cis-1,3-DACH)Pt(5′GMP)₂ and (cis-1,3-DACH)Pt(3′GMP)₂ adducts show preference for the ΛHT and ∆HT conformations, respectively (HT stands for Head-to-Tail). Moreover, the increased intensity of the circular dichroism signals in the cis-1,3-DACH derivatives with respect to the analogous cis-(NH₃)₂ species could be a consequence of the greater bite angle of the cis-1,3-DACH carrier ligand with respect to cis-(NH₃)₂. Finally, the (cis-1,3-DACH)Pt(d(GpG)) adduct is present in two isomeric forms, each one giving a pair of H8 resonances linked by a NOE cross peak. The two isomers were formed in comparable amounts and had a dominance of the HH conformer but with some contribution of the ΔHT conformer which is related to the HH conformer by having the 3′-G base flipped with respect to the 5′-G residue. Full article

Among climate-change related effects, drought, heat, and waterlogging are the most important adversely affecting the production of potatoes in Europe. As climate change progresses, agricultural practices must adapt to maintain potato yields. This study is based on a European-wide survey. It presents potato growers’ perception of climate change, its impact, and possible adaptation strategies, focusing on the results from Germany, Switzerland, and Austria. Potato growers strongly agreed that climate change had affected their potato production in the last ten years, as indicated by 98% of German and more than 90% of Swiss and Austrian respondents. Drought caused the most severe impact, and to varying extents damage was caused by heat and the occurrence of pests and pathogens. The most preferred adaptation measure was the planting of adapted varieties. In line with the comparably low access to at least partial irrigation that Austrian potato growers reported, Austria appeared to be the country most affected by drought. Other more pronounced challenges were late spring frost, flash floods, and soil erosion. The study highlights and discusses specific differences between the countries, as well as between conventional and organic potato production based on the Austrian responses. The results underline that to successfully develop effective climate change mitigation strategies, country-specific and local challenges and needs should be considered. Full article

The DACH geographic region comprises three countries in central Europe: Germany, Austria, and Switzerland. This study seeks to both understand the motivations for purchasing organic and sustainably certified wines in this region and identifies which type of consumer should be targeted for the continued growth of this wine sector. Our methodology includes an online questionnaire resulting in a sample size of 4553 respondents, evenly broken down by country. Our analysis includes simple statistics and more advanced statistical analysis of the dataset. Our results indicate that there is a correlation between wine knowledge, environmental awareness, and the likelihood of buying eco-certified wines. We find that those who are self-proclaimed wine experts have a higher willingness to pay for organic or sustainable certified wines. These customers are generally middle aged, most often male, live in multi-person households, have pursued some level of higher education, and many are self-employed. We believe this study captures the growing trend of organic and sustainable wine certifications. It also identifies who is already convinced and who will need more marketing/education before they are ready to buy organic or sustainable wine. Full article

Lethal toxin (LT) is the critical virulence factor of Bacillus anthracis, the causative agent of anthrax. One common symptom observed in patients with anthrax is thrombocytopenia, which has also been observed in mice injected with LT. Our previous study demonstrated that LT induces thrombocytopenia by suppressing megakaryopoiesis, but the precise molecular mechanisms behind this phenomenon remain unknown. In this study, we utilized 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced megakaryocytic differentiation in human erythroleukemia (HEL) cells to identify genes involved in LT-induced megakaryocytic suppression. Through cDNA microarray analysis, we identified Dachshund homolog 1 (DACH1) as a gene that was upregulated upon TPA treatment but downregulated in the presence of TPA and LT, purified from the culture supernatants of B. anthracis. To investigate the function of DACH1 in megakaryocytic differentiation, we employed short hairpin RNA technology to knock down DACH1 expression in HEL cells and assessed its effect on differentiation. Our data revealed that the knockdown of DACH1 expression suppressed megakaryocytic differentiation, particularly in polyploidization. We demonstrated that one mechanism by which B. anthracis LT induces suppression of polyploidization in HEL cells is through the cleavage of MEK1/2. This cleavage results in the downregulation of the ERK signaling pathway, thereby suppressing DACH1 gene expression and inhibiting polyploidization. Additionally, we found that known megakaryopoiesis-related genes, such as FOSB, ZFP36L1, RUNX1, FLI1, AHR, and GFI1B genes may be positively regulated by DACH1. Furthermore, we observed an upregulation of DACH1 during in vitro differentiation of CD34–megakaryocytes and downregulation of DACH1 in patients with thrombocytopenia. In summary, our findings shed light on one of the molecular mechanisms behind LT-induced thrombocytopenia and unveil a previously unknown role for DACH1 in megakaryopoiesis. Full article

Background and objectives: Similar to saturated fat, a diet rich in sugar may contribute to the development of overweight and obesity and associated metabolic diseases, like type 2 diabetes and metabolic dysfunction associated steatotic liver disease (MASLD). Herein, effects on intestinal microbiota composition and barrier function subsequently leading to an increased translocation of bacterial endotoxin and activation of Toll-like receptor (TLR) 4-dependent signaling cascade are discussed to be critical. In recent years, the use of artificial sweeteners to sweeten food and beverages has markedly increased despite a still limited knowledge on health effects. Results of animal studies suggest that an extended intake of sweeteners like sucralose may alter intestinal microbiota composition and gut barrier function when consumed at high levels. In the present pilot study, we assessed the effects of an acute intake of sucrose and the artificial sweetener sucralose in physiological relevant doses in beverages on postprandial endotoxemia in healthy, normal-weight young adults. Methods: A total of 11 men and women aged 24–31 year were enrolled in this randomized placebo controlled single-blinded study in cross-over design which was approved by the ethics committee of the University of Vienna (Clinical trial: NCT04788680). After an initial blood collection and a 2 day nutritional standardization, according to the recommendations of the German, Austrian and Swiss (DACH) nutritional societies, and a second fasted blood collection, participants consumed either a beverage containing sucrose (110 g), sucralose (180 mg, iso-sweet) or an isocaloric combination of sucralose (180 mg) + maltodextrin (110 g) in a randomized order along with a standardized breakfast. Blood was collected 1, 2 and 3 h after consumption of the beverage. Bacterial endotoxin levels in plasma were measured using LAL assay. Results: After nutritional standardization, bacterial endotoxin levels were significantly lower than before. Furthermore, 2 h after the intake of the sucrose sweetened beverage, bacterial endotoxin levels were significantly higher in plasma compared to baseline levels. A similar increase in bacterial endotoxin levels in plasma was not detected after the intake of the beverage sweetened with sucralose. Discussion: Our data suggest that the intake of a sucrose but not sucralose sweetened beverage results in post-prandial endotoxemia. Full article