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Keywords = Cer-cqu gene cluster

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17 pages, 2844 KB  
Article
WAX INDUCER 1 Regulates β-Diketone Biosynthesis by Mediating Expression of the Cer-cqu Gene Cluster in Barley
by Sophia V. Gerasimova, Ekaterina V. Kolosovskaya, Alexander V. Vikhorev, Anna M. Korotkova, Christian W. Hertig, Mikhail A. Genaev, Dmitry V. Domrachev, Sergey V. Morozov, Elena I. Chernyak, Nikolay A. Shmakov, Gennady V. Vasiliev, Alex V. Kochetov, Jochen Kumlehn and Elena K. Khlestkina
Int. J. Mol. Sci. 2023, 24(7), 6762; https://doi.org/10.3390/ijms24076762 - 5 Apr 2023
Cited by 10 | Viewed by 4631
Abstract
Plant surface properties are crucial determinants of resilience to abiotic and biotic stresses. The outer layer of the plant cuticle consists of chemically diverse epicuticular waxes. The WAX INDUCER1/SHINE subfamily of APETALA2/ETHYLENE RESPONSIVE FACTORS regulates cuticle properties in plants. In this study, four [...] Read more.
Plant surface properties are crucial determinants of resilience to abiotic and biotic stresses. The outer layer of the plant cuticle consists of chemically diverse epicuticular waxes. The WAX INDUCER1/SHINE subfamily of APETALA2/ETHYLENE RESPONSIVE FACTORS regulates cuticle properties in plants. In this study, four barley genes homologous to the Arabidopsis thaliana AtWIN1 gene were mutated using RNA-guided Cas9 endonuclease. Mutations in one of them, the HvWIN1 gene, caused a recessive glossy sheath phenotype associated with β-diketone deficiency. A complementation test for win1 knockout (KO) and cer-x mutants showed that Cer-X and WIN1 are allelic variants of the same genomic locus. A comparison of the transcriptome from leaf sheaths of win1 KO and wild-type plants revealed a specific and strong downregulation of a large gene cluster residing at the previously known Cer-cqu locus. Our findings allowed us to postulate that the WIN1 transcription factor in barley is a master mediator of the β-diketone biosynthesis pathway acting through developmental stage- and organ-specific transactivation of the Cer-cqu gene cluster. Full article
(This article belongs to the Special Issue Advances in Molecular Plant Sciences)
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18 pages, 2020 KB  
Review
The Polyketide Components of Waxes and the Cer-cqu Gene Cluster Encoding a Novel Polyketide Synthase, the β-Diketone Synthase, DKS
by Penny Von Wettstein-Knowles
Plants 2017, 6(3), 28; https://doi.org/10.3390/plants6030028 - 10 Jul 2017
Cited by 14 | Viewed by 6351
Abstract
The primary function of the outermost, lipophilic layer of plant aerial surfaces, called the cuticle, is preventing non-stomatal water loss. Its exterior surface is often decorated with wax crystals, imparting a blue–grey color. Identification of the barley Cer-c, -q and -u genes [...] Read more.
The primary function of the outermost, lipophilic layer of plant aerial surfaces, called the cuticle, is preventing non-stomatal water loss. Its exterior surface is often decorated with wax crystals, imparting a blue–grey color. Identification of the barley Cer-c, -q and -u genes forming the 101 kb Cer-cqu gene cluster encoding a novel polyketide synthase—the β-diketone synthase (DKS), a lipase/carboxyl transferase, and a P450 hydroxylase, respectively, establishes a new, major pathway for the synthesis of plant waxes. The major product is a β-diketone (14,16-hentriacontane) aliphatic that forms long, thin crystalline tubes. A pathway branch leads to the formation of esterified alkan-2-ols. Full article
(This article belongs to the Special Issue The Plant Cuticle)
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