Search Results (6)

This study investigates the Huangdong Daning River Bridge project in Guangxi, where the innovative side-span and mid-span synchronous closure technology for continuous rigid-frame bridges (CRFB) was systematically implemented for the first time in this region of China. A comparative finite element model developed in MIDAS Civil 2024 was employed to analyze the mechanical behavior mechanisms of main girders under span-by-span closure and synchronous closure processes. The numerical simulation results demonstrate that the stress distribution in main girders shows no significant sensitivity (<3%) to closure method differences during both the bridge completion phase and 10-year shrinkage-creep cycle. However, distinct closure sequences (asynchronous vs. synchronous) exhibited notable impacts on the girder alignment at the completion stage. The cumulative deviation induced by differential installation elevations of formwork segments necessitates precise dynamic control during construction monitoring. Furthermore, shrinkage and creep effects manifested differential influences on pier top horizontal displacements and bending moments when employing different closure methods, though all variations remained within 5%. The synchronous multi-span closure technology effectively mitigates structural mutation risks during construction while achieving superior alignment accuracy, rational stress distribution, and accelerated construction progress as verified by field implementation. Full article

Continuous rigid-framed bridges with super-high piers (CRFB-HP) have been widely applied in mountain areas. However, their seismic performance is still urgently to be clarified. In this study, the refined finite element model (FEM) of a CRFB-HP was constructed and verified according to the shaking table test results of its scaled model. On this basis, systematic elastic-plastic time history analysis of the CRFB-HP was conducted to investigate the influence of parameters on their seismic performance, including main bridge span, pier height and number of tie beams. The results show that CRFB-HP have the characteristic of long vibration periods and are more sensitive to long-period ground motions. Along the longitudinal and transverse directions, the peak pier top displacement and pier bottom bending moment of CRFB-HP are both relatively large under NLPL (+20~+70%) and NFPT (T_P ≈ T₁, +50~+120%) excitations. For the same span, the peak pier top displacement increases with the pier height increasing, while the peak pier bottom bending moment decreases with the pier height increasingFor the same pier height, the peak pier top displacement and peak pier bottom bending moment both increase with the span length increasing. Moreover, the pier height change has a greater effect on the pier top displacement than that of the span change. CRFB-HP show obvious high-order response participation (HRP) under different ground motions. The NFPT (T_P ≈ T₁), ground motions can significantly increase HRP. Moreover, compared with cast-in-place CRFB-HP, the HRP of a fabricated super-high pier is greater (+20~+30%). The peak pier top displacement and pier bottom bending moment both decrease with the increase in the number of tie beams. The reasonable arrangement of tie beams can improve the lateral seismic performance of CRFB-HP. However, compared to the cast-in-place CRFB-HP, the peak pier top displacement is larger, and the peak pier bottom bending moment is smaller, for the fabricated CRFB-HP. Full article

In aquaculture, viral diseases pose a significant threat and can lead to substantial economic losses. The primary defense against viral invasion is the innate immune system, with interferons (IFNs) playing a crucial role in mediating the immune response. With advancements in molecular biology, the role of non-coding RNA (ncRNA), particularly microRNAs (miRNAs), in gene expression has gained increasing attention. While the function of miRNAs in regulating the host immune response has been extensively studied, research on their immunomodulatory effects in teleost fish, including silver carp (Hyphthalmichthys molitrix), is limited. Therefore, this research aimed to investigate the immunomodulatory role of microRNA-30b-5p (miR-30b-5p) in the antiviral immune response of silver carp (Hypophthalmichthys molitrix) by targeting cytokine receptor family B5 (CRFB5) via the JAK/STAT signaling pathway. In this study, silver carp were stimulated with polyinosinic-polycytidylic acid (poly (I:C)), resulting in the identification of an up-regulated miRNA (miR-30b-5p). Through a dual luciferase assay, it was demonstrated that CRFB5, a receptor shared by fish type I interferon, is a novel target of miR-30b-5p. Furthermore, it was found that miR-30b-5p can suppress post-transcriptional CRFB5 expression. Importantly, this study revealed for the first time that miR-30b-5p negatively regulates the JAK/STAT signaling pathway, thereby mediating the antiviral immune response in silver carp by targeting CRFB5 and maintaining immune system stability. These findings not only contribute to the understanding of how miRNAs act as negative feedback regulators in teleost fish antiviral immunity but also suggest their potential therapeutic measures to prevent an excessive immune response. Full article

IFNAR1, one of the type I IFN receptors, is crucial to mammalian host defense against viral invasion. However, largely unknown is the immunological role of the fish teleost protein IFNAR1, also known as CRFB5. We have successfully cloned the whole cDNA of the Japanese eel’s (Anguilla japonica) CRFB5a homolog, AjCRFB5a. The two fibronectin-3 domains and the transmembrane region (238–260 aa) of AjCRFB5a are normally present, and it shares a three-dimensional structure with zebrafish, Asian arowana, and humans. According to expression analyses, AjCRFB5a is highly expressed in all tissues found, particularly the liver and intestine. In vivo, Aeromonas hydrophila, LPS, and the viral mimic poly I:C all dramatically increased AjCRFB5a expression in the liver. Japanese eel liver cells were reported to express AjCRFB5a more strongly in vitro after being exposed to Aeromonas hydrophila or being stimulated with poly I: C. The membranes of Japanese eel liver cells contained EGFP-AjCRFB5a proteins, some of which were condensed, according to the results of fluorescence microscopy. Luciferase reporter assays showed that AjCRFB5a overexpression strongly increased the expression of immune-related genes in Japanese eel liver cells, such as IFN1, IFN2, IFN3, IFN4, IRF3, IRF5, and IRF7 of the type I IFN signaling pathway, as well as one of the essential antimicrobial peptides LEAP2, in addition to significantly inducing human IFN-promoter activities in HEK293 cells. Additionally, RNA interference (RNAi) data demonstrated that knocking down AjCRFB5a caused all eight of those genes to drastically lower their expression in Japanese eel liver cells, as well as to variable degrees in the kidney, spleen, liver, and intestine. Our findings together showed that AjCRFB5a participates in the host immune response to bacterial infection by inducing antimicrobial peptides mediated by LEAP2 and favorably modulates host antiviral immune responses by activating IRF3 and IRF7-driven type I IFN signaling pathways. Full article

Objective: The aim of this study was to determine whether modern congruent tibial inserts are associated with superior outcomes in total knee arthroplasty (TKA). Background: Ultracongruent fixed-bearing (UCFB) and medial congruent fixed-bearing (MCFB) inserts have been known to be effective in total knee arthroplasty with patient satisfaction. Nonetheless, no supporting evidence to date exists to rank the clinical outcomes of these various congruent inserts in TKA compared with other important considerations in TKA including cruciate-retaining fixed-bearing (CRFB) and posterior-stabilized fixed-bearing (PSFB) inserts. Methods: We searched PubMed, Embase, the Cochrane Central Register of Controlled Trials, Web of Science, and Scopus up to 15 May 2022. We selected studies involving an active comparison of UCFB or MCFB in TKAs. We performed a network meta-analysis (NMA) of randomized controlled trials (RCTs) and compared different congruent inserts. We ranked the clinical outcomes by SUCRA score with the estimate of the best treatment probability. Our primary outcomes were revision rates and radiolucent lines. Secondary outcomes were functional scores, including the range of motion (ROM), the Knee Society Score (KSS), the Oxford Knee Score (OKS), and WOMAC. Results: Eighteen RCTs with 1793 participants were analyzed. Our NMA ranked MCFB, CRFB, and UCFB with the lowest revision rates. CRFB and UCFB had the fewest radiolucent lines. UCFB had overall the best ROM. UCFB and MCFB had the best OKS score overall. Conclusions: The ranking probability for better clinical outcomes in congruent inserts demonstrated the superiority of congruent tibial inserts, including UCFB and MCFB. UCFB may be associated with better ROM and postoperative functional outcomes. However, integrating future RCTs for high-level evidence is necessary to confirm these findings. Full article

The dynamic characteristics of a continuous rigid-frame bridge with fabricated super-high piers (CRFB-FSP) connected by grouting sleeves and adjacent continuous beam bridges (AB) are significantly different, and they are prone to pounding under earthquake excitation. At present, the pounding response between the CRFB-FSP and AB is still unclear, and the impact of the pounding on the seismic performance of a CRFB-FSP is still in the exploratory stage. In this study, two 1/20 scaled models of a CRFB-FSP (MB) and a cast-in-place AB were designed and manufactured. Then, according to the research purpose and the output performance of the shaking table, three each of non-long-period (NLP) ground motions and near-fault pulse-type (NFPT) ground motions were selected as the inputs of the excitation shaking table test. The peak ground acceleration (PGA) changes from 0.5 g to 1.5 g. According to the similarity ratio (1/20), the initial gap between the MB and AB was taken as 7 mm (prototype design: 140 mm). Furthermore, the longitudinal pounding response between the CFRB-FSP and AB, as well as its influence on the seismic performance of the CFRB-FSP, was systematically investigated through a shaking table test and finite element analysis (FEA). The results showed that the pounding with the CRFB-FSP easily caused a persistent pounding, which may increase the damage risk of the pier. The peak pounding force under the NFPT ground motion was more significant than under the NLP ground motion, whereas the pounding number under the NFPT ground motion was smaller. The peak pounding force increased with the increase in the initial gap, pounding stiffness, span, and pier height. With and without pounding, the CRFB-FSP reflected higher-order mode participation (HMP) characteristics. After pounding, under the NFPT excitation, the HMP contribution increased significantly compared with that of the without pounding condition, while this effect under the NLP excitation was smaller. The peak displacement of the main beam of the CRFB-FSP increased with the increase in the main beam span, pier height and initial gap. The peak bending moment of the pier bottom increased with the increase in the main beam span and initial gap, however, decreased with the increase in the pier height. Moreover, the peak displacement of the main beam and the peak moment of the pier bottom of the CRFB-FSP both reduced. In contrast, the corresponding seismic response of the AB increased under the same conditions. Full article