Search Results (5)

Qualitative visual clutch staging is a useful tool for rapidly and non-invasively assessing the developmental stage of American lobster, Homarus americanus, embryos. While such a scheme has been used in fisheries monitoring strategies in Canada since the 1980s, the biological relevance of its four visually distinguishable stages is poorly understood. We conducted a laboratory experiment in which 10 ovigerous females were housed and the development of their embryos regularly assessed, both qualitatively and quantitatively, from November until hatching in July/August. We confirmed the biological relevance of the qualitative staging scheme by showing clear quantitative differences in the duration and rate of embryonic development of stages 2–4 (stage 1 was not assessed as the precise spawning date was unknown). Stage 2 represents winter–spring “dormancy”. Stage 3 represents a shorter period of rapid development preceding hatch. Stage 4 represents hatching. We also recommend some improvements to the qualitative staging scheme, specifically (1) adding criteria related to the portion of eggs that are occupied by yolk to increase the accuracy of staging, (2) slightly redefining stage 3 to ensure it encompasses the full period of rapid embryonic development pre-hatch, and (3) adding the presence of pre-zoeae as a key indicator of hatching to avoid the misclassification of clutches in the early stages of hatching or those that are completely spent but still have adhesive substance. Full article

The American lobster (Homarus americanus) is the most valuable seafood on Canada’s Atlantic coast, generating over CAD 800 million in export revenue alone for New Brunswick. However, labor shortages plague the lobster industry, and lobsters must be processed quickly to maintain food safety and quality assurance standards. This paper proposes a lobster estimation orientation approach using a convolutional neural network model, with the aim of guiding the FANUC LR Mate 200 iD robotic arm for lobster manipulation. To validate this technique, four state-of-the-art object detection algorithms were evaluated on an American lobster images dataset: YOLOv7, YOLOv7-tiny, YOLOV4, and YOLOv3. In comparison to other versions, YOLOv7 demonstrated a superior performance with an F1-score of 95.2%, a mean average precision (mAP) of 95.3%, a recall rate of 95.1%, and 111 frames per second (fps). Object detection models were deployed on the NVIDIA Jetson Xavier NX, with YOLOv7-tiny achieving the highest fps rate of 25.6 on this platform. Due to its outstanding performance, YOLOv7 was selected for developing lobster orientation estimation. This approach has the potential to improve efficiency in lobster processing and address the challenges faced by the industry, including labor shortages and compliance with food safety and quality standards. Full article

Bentho-pelagic life cycles are the dominant reproductive strategy in marine invertebrates, providing great dispersal ability, access to different resources, and the opportunity to settle in suitable habitats upon the trigger of environmental cues at key developmental moments. However, free-dispersing larvae can be highly sensitive to environmental changes. Among these, the magnitude and the occurrence of elevated carbon dioxide (CO₂) concentrations in oceanic habitats is predicted to exacerbate over the next decades, particularly in coastal areas, reaching levels beyond those historically experienced by most marine organisms. Here, we aimed to determine the sensitivity to elevated pCO₂ of successive life stages of a marine invertebrate species with a bentho-pelagic life cycle, exposed continuously during its early ontogeny, whilst providing in-depth insights on their metabolic responses. We selected, as an ideal study species, the American lobster Homarus americanus, and investigated life history traits, whole-organism physiology, and metabolomic fingerprints from larval stage I to juvenile stage V exposed to different pCO₂ levels. Current and future ocean acidification scenarios were tested, as well as extreme high pCO₂/low pH conditions that are predicted to occur in coastal benthic habitats and with leakages from underwater carbon capture storage (CCS) sites. Larvae demonstrated greater tolerance to elevated pCO₂, showing no significant changes in survival, developmental time, morphology, and mineralisation, although they underwent intense metabolomic reprogramming. Conversely, juveniles showed the inverse pattern, with a reduction in survival and an increase in development time at the highest pCO₂ levels tested, with no indication of metabolomic reprogramming. Metabolomic sensitivity to elevated pCO₂ increased until metamorphosis (between larval and juvenile stages) and decreased afterward, suggesting this transition as a metabolic keystone for marine invertebrates with complex life cycles. Full article

Central pattern generators produce rhythmic behaviors independently of sensory input; however, their outputs can be modulated by neuropeptides, thereby allowing for functional flexibility. We investigated the effects of C-type allatostatins (AST-C) on the cardiac ganglion (CG), which is the central pattern generator that controls the heart of the American lobster, Homarus americanus, to identify the biological mechanism underlying the significant variability in individual responses to AST-C. We proposed that the presence of multiple receptors, and thus differential receptor distribution, was at least partly responsible for this observed variability. Using transcriptome mining and PCR-based cloning, we identified four AST-C receptors (ASTCRs) in the CG; we then characterized their cellular localization, binding potential, and functional activation. Only two of the four receptors, ASTCR1 and ASTCR2, were fully functional GPCRs that targeted to the cell surface and were activated by AST-C peptides in our insect cell expression system. All four, however, were amplified from CG cDNAs. Following the confirmation of ASTCR expression, we used physiological and bioinformatic techniques to correlate receptor expression with cardiac responses to AST-C across individuals. Expression of ASTCR1 in the CG showed a negative correlation with increasing contraction amplitude in response to AST-C perfusion through the lobster heart, suggesting that the differential expression of ASTCRs within the CG is partly responsible for the specific physiological response to AST-C exhibited by a given individual lobster. Full article

The growing transportation of petroleum products pose a significant risk of marine diesel or diluted bitumen (dilbit) spills at sea. Despite the economic importance of the American lobster, there have been few studies assessing the impact study of such a spill on their population. In the lobster industry, lobster quality is monitored according to the Brix index of hemolymph. In our research, the effectiveness of three other biomarkers operative in the industry was assessed in hemolymph during contamination (over 96 h) by marine diesel and dilbit (Cold Lake Blend; CLB), as well as in the subsequent recovery period, according to two temperature cycles. At the end of the experiment, chemical and tainting assays were performed. Our results demonstrate that, among the four tested biomarkers, lysosomal stability and ethoxyresorufin O-deethylase (EROD) induction exhibit higher sensitivity. Increasing the temperature did not shorten the recovery period. Viability cellular impacts were greater in lobsters exposed to dilbit than that in those exposed to marine diesel. Marine diesel exposure appears to be more problematic for the lobster fishery, as the cooked lobster meat still presented a hydrocarbon odor even after 3 months of live holding. Finally, the high PAH concentrations measured in lobster eggs suggest potential adverse transgenerational effects of marine diesel exposure. Full article