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Keywords = Amazon biobank

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12 pages, 3685 KB  
Article
Establishment and Characterization of a Primary Fibroblast Cell Culture from the Amazonian Manatee (Trichechus inunguis)
by Flávia dos Santos Tavares, Cesar Martins, Flávia Karina Delella, Luís Adriano Santos do Nascimento, Angélica Lúcia Figueiredo Rodrigues, Sávia Moreira, Adauto Lima Cardoso and Renata Coelho Rodrigues Noronha
Animals 2024, 14(5), 686; https://doi.org/10.3390/ani14050686 - 22 Feb 2024
Cited by 6 | Viewed by 4250
Abstract
The vulnerable status of the Amazon manatee, Trichechus inunguis, indicates the need to seek measures to guarantee its conservation. In this context, the cultivation of cells in vitro is a strategy that should at least guarantee the preservation of their genetic material. [...] Read more.
The vulnerable status of the Amazon manatee, Trichechus inunguis, indicates the need to seek measures to guarantee its conservation. In this context, the cultivation of cells in vitro is a strategy that should at least guarantee the preservation of their genetic material. Thus, we established for the first time a primary culture of Amazonian manatee fibroblasts (TINsf) from a skin biopsy of a young male. Karyotypic analysis of the 3rd, 7th, and 12th passages confirmed the taxonomic identity of the species T. inunguis (2n = 56/NF = 92) and indicated that this culture presents genomic stability. Gene and protein expression of vimentin at the 13th passage show the predominant presence of fibroblasts in TINsf. To test the cell line’s responsiveness to materials and demonstrate a possible application of this culture, it was exposed to andiroba seed oil (ASO), and its viability and proliferative capacity were evaluated. ASO demonstrated toxic effects at the highest concentrations and longest exposure times tested, reproducing results observed in human cultures, indicating the applicability of TINsf in toxicological and biotechnological studies. After cryopreservation, the TINsf line maintained its proliferative potential, indicating the establishment of a new culture available for future studies. Full article
(This article belongs to the Section Mammals)
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19 pages, 593 KB  
Article
Public Trust in Health Information Sharing: Implications for Biobanking and Electronic Health Record Systems
by Jodyn Platt and Sharon Kardia
J. Pers. Med. 2015, 5(1), 3-21; https://doi.org/10.3390/jpm5010003 - 3 Feb 2015
Cited by 66 | Viewed by 13664
Abstract
Biobanks are made all the more valuable when the biological samples they hold can be linked to health information collected in research, electronic health records, or public health practice. Public trust in such systems that share health information for research and health care [...] Read more.
Biobanks are made all the more valuable when the biological samples they hold can be linked to health information collected in research, electronic health records, or public health practice. Public trust in such systems that share health information for research and health care practice is understudied. Our research examines characteristics of the general public that predict trust in a health system that includes researchers, health care providers, insurance companies and public health departments. We created a 119-item survey of predictors and attributes of system trust and fielded it using Amazon’s MTurk system (n = 447). We found that seeing one’s primary care provider, having a favorable view of data sharing and believing that data sharing will improve the quality of health care, as well as psychosocial factors (altruism and generalized trust) were positively and significantly associated with system trust. As expected, privacy concern, but counterintuitively, knowledge about health information sharing were negatively associated with system trust. We conclude that, in order to assure the public’s trust, policy makers charged with setting best practices for governance of biobanks and access to electronic health records should leverage critical access points to engage a diverse public in joint decision making. Full article
(This article belongs to the Special Issue Biobanking and EHR/EMR)
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