A new ferulic acid ester named 4-methyl-3-
trans-hexenylferulate (
1), together with eight known phenolic acid esters (
2–
9), was isolated from the methanolic extract of the roots and rhizomes of
Notopterygium incisium. Their structures were elucidated
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A new ferulic acid ester named 4-methyl-3-
trans-hexenylferulate (
1), together with eight known phenolic acid esters (
2–
9), was isolated from the methanolic extract of the roots and rhizomes of
Notopterygium incisium. Their structures were elucidated by extensive spectroscopic techniques, including 2D NMR spectroscopy and mass spectrometry. 4-Methoxyphenethyl ferulate (
8) NMR data is reported here for the first time. The uptake and transepithelial transport of the isolated compounds
1–
9 were investigated in the human intestinal Caco-2 cell monolayer model. Compounds
2 and
6 were assigned for the well-absorbed compounds, compound
8 was assigned for the moderately absorbed compound, and compounds
1,
3,
4,
5,
7, and
9 were assigned for the poorly absorbed compounds. Moreover, all of the isolated compounds were assayed for the inhibitory effects against nitric oxide (NO) production in the lipopolysaccharide-activated RAW264.7 macrophages model and
L-N6-(1-iminoethyl)-lysine (L-NIL) was used as a positive control. Compounds
1,
5,
8, and
9 exhibited potent inhibitory activity on NO production with the half maximal inhibitory concentration (IC
50) values of 1.01, 4.63, 2.47, and 2.73 μM, respectively, which were more effective than L-NIL with IC
50 values of 9.37 μM. These findings not only enriched the types of anti-inflammatory compounds in
N. incisum but also provided some useful information for predicting their oral bioavailability and their suitability as drug leads or promising anti-inflammatory agents.
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