Search Results (4)

This study provides a thorough examination of the utilization of hydrophobic deep eutectic solvents (HDESs) for the extraction of 1,2-dichloroethane (1,2-DCA) from effluent, with an emphasis on a sustainable and environmentally friendly approach. The extraction efficacy of six HDES systems was initially evaluated, and the combinations of thymol/camphor (Thy/Cam) and menthol/thymol (Men/Thy) exhibited superior performance. Subsequently, these two HDESs were chosen for a comprehensive parametric analysis. The impact of contact time demonstrated that extraction equilibrium was reached at 15 min for both systems, thereby achieving a balance between high efficiency and time efficiency. Next, the impact of the HDES-to-water mass ratio was investigated. A 1:1 ratio was determined to be the most effective, as it minimized solvent consumption and provided high efficiency. An additional examination of the molar ratios of the HDES components revealed that the 1:1 ratio exhibited the most effective extraction performance. This was due to the fact that imbalances in the solvent mixture resulted in diminished efficiency as a result of disrupted molecular interactions. The extraction efficiency was significantly influenced by the initial concentration of 1,2-DCA, with higher concentrations resulting in superior results as a result of the increased mass transfer driving forces. In general, the Men/Thy and Thy/Cam systems have shown noteworthy stability and efficiency under different conditions, which makes them highly suitable for large-scale applications. Full article

This study aimed to explore the microbial communities present in aquifer groundwater at a petrochemical refinery and their relationship with groundwater quality parameters, with a focus on common contaminants such as benzene, toluene, ethylbenzene, and dichloroethane (DCA). Groundwater samples were collected from both the source and plume regions to analyze the spatial diversity of the microbial communities utilizing 16S rRNA analysis. The study demonstrated substantial variations in microbial diversity and composition across the sampled sites. The data showed that the operational taxonomic unit count, Shannon index, and Simpson index initially rose before declining with escalating contaminant concentration, suggesting that the level of contaminants significantly influences the abundance and diversity of microbial communities in the phreatic groundwater. Moreover, through SPSS analysis, the study quantitatively established the correlation between the physiochemical characteristics of the groundwater and the microbial community structure. The study disclosed that geochemical parameters, including total alkalinity, ferrous content, and DCA, play a role in shaping the abundance and diversity of microbial communities at the phylum, class, and genus levels. This research contributes to our comprehension of the intricate interplay between microbial communities, particularly those implicated in the biotransformation of benzene and DCA, and their surrounding physiochemical milieu within contaminated zones. Full article

Contamination of soil and groundwater by chlorinated solvents is an environmental issue of primary concern. Recently, electrically conductive iron particles have been proposed as a novel approach to accelerate anaerobic bioremediation processes. In fact, it was demonstrated that conductive particles facilitate the exchange of electrons between microorganisms via Direct Interspecies Electron Transfer (DIET) processes, thus enhancing the pollutant-degrading potential of the microbial community. However, the use of natural minerals in this context has not been reported so far. In this study, we applied, for the first time, natural magnetite and hematite to accelerate the reductive dechlorination of 1,2-dichloroethane by an enrichment culture in lab-scale anaerobic microcosms. After four feeding cycles, low magnetite-amended microcosms (13 mg/L) yielded the highest rate of 1,2-DCA reductive dechlorination and reduced methanogenic activity. By contrast, hematite did not display any apparent stimulatory effect. Surprisingly, in the presence of higher amounts of iron oxides, a weaker effect was obtained, probably because iron(III) present in the minerals competed for the electrons necessary for reductive dechlorination. For all microcosms, the concentration of the toxic byproduct vinyl chloride was negligible throughout the whole study. The SEM/EDS analysis confirmed the close interaction between the conductive iron oxide particles and the dechlorinating bacteria. This work opens the possibility of using natural conductive minerals for bioremediation applications as well as shedding light on the previously unrecognized role of such minerals in contaminated ecosystems. Full article

Natural attenuation (NA) processes represent a valuable option in groundwater remediation. At a heavily 1,2-dichloroethane (1,2-DCA) contaminated site, Compound-Specific Isotope Analysis (CSIA) in combination with Biological Molecular Tools (BMTs) were implemented as a rigorous characterization approach to evaluate the occurrence of Natural Attenuation in the proximity of the source area. By the use of microcosm experiments, the potential for natural and enhanced biodegradation under anaerobic conditions was documented, following the dichloroelimination pathway. Enrichment factors of −9.1‰ and −11.3‰ were obtained for ¹³C while Geobacter spp. and reductive dehalogenase genes (rdhs) were identified as main site-specific biomarkers. At pilot scale, enrichments of 13.5‰ and 6.3‰ for δ¹³C and δ³⁷Cl, respectively, high levels of reductive dehalogenase (rdh group VI) along with the dominance of Geobacter spp. indicated the occurrence of significant dichloroelimination processes in groundwater under anaerobic conditions. By using the site-specific enrichment factors, degradation extents over approximately 70–80% were estimated, highlighting the relevant potential of NA in 1,2-DCA degradation in the vicinity of the source area at the site. The proposed fine-tuned protocol, including CSIA and BMTs, is proven to be effective as a groundwater remediation strategy, properly assessing and monitoring NA at site scale. Full article