Search Results (8)

Heavy metal and microbial pollution in groundwater raises health concerns due to its adverse effects. This study aimed to assess the health risks associated with heavy metal and bacterial pollution in groundwater in Mankweng. Heavy metals and Escherichia coli were detected using inductively coupled plasma mass spectrophotometry and a Colilert system, respectively. The heavy metal pollution index (HPI) and non-carcinogenic and carcinogenic risks were computed. The β-Poisson dose–response model was employed to predict the probability of E. coli infection. The metals’ concentrations were all within the permissible limits of the South African National Standard (SANS). However, Pb levels at sites E and G exceeded the World Health Organization (WHO) guideline (≤0.01 mg/L). HPI values were all less than 100, indicative of low contamination. The hazard quotient values were all less than 1, except for vanadium. The cumulative cancer risk ranged between 3.06 × 10⁻⁵ and 1.81 × 10⁻⁴ and between 3.55 × 10⁻⁵ and 2.20 × 10⁻⁴ for adults and children, respectively. E. coli was only detected at site L. The annual risk of E. coli infection exceeded the WHO risk limit of 10⁻⁴. The results underscore the need for the regular monitoring of groundwater. Full article

There is a lack of groundwater quality monitoring, especially in developing countries like South Africa. This study aimed to evaluate borehole water quality. Groundwater was analysed for pH, dissolved oxygen (DO), temperature, electrical conductivity (EC), total dissolved solids (TDSs), turbidity, chemical oxygen demand (COD), nitrogen (N), sulphate (SO₄²⁻), fluoride (F⁻), chloride (Cl⁻), calcium (Ca²⁺), magnesium (Mg²⁺), potassium (K⁺), and sodium (Na⁺) using a multi-parameter device, spectrophotometer, turbidity meter, and inductively coupled plasma optical emission spectrophotometer. Total coliforms and Escherichia coli were quantified using the Colilert system. The water quality index (WQI) was calculated using the arithmetic weighting method. The parameters ranged as follows: pH (6.71–7.94), DO (2.19–7.79 mg/L), EC (379.67–1317.33 µS/cm), TDSs (190–659 mg/L), temperature (16.75–22.31 °C), turbidity (0.17–3.21 NTU), COD (9–50 mg/L), F⁻ (0.17–2.09 mg/L), Cl⁻ (36.1–184.55 mg/L), N (0.64–28.56 mg/L), SO₄²⁻ (27.18–112.13 mg/L), K⁺ (1.71–21.77 mg/L), Ca²⁺ (29.59–134.59 mg/L), Mg²⁺ (16.72–110.78 mg/L), and Na⁺ (38.52–170.63 mg/L). One borehole was polluted with E. coli (9 MPN/100 mL) and 25% were contaminated with coliforms beyond 10 MPN/100 mL. The WQI ranged from 50.430 to 190.220. The results underscore the importance of regular monitoring of groundwater. Full article

Aloe arborescens Mill’s extracts have been explored for antibacterial and antioxidant efficacies. However, there is limited information on its chemical composition and mechanism of action. The purpose of this study was to assess the chemical composition, antibacterial and antioxidant activities and mechanism of the whole leaf extract of A. arborescens Mill. The phytochemical profile was analysed with gas chromatography mass spectrometry (GC-MS). The antioxidant and antibacterial activities were screened using 1,1diphenyl2picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and micro-dilution assays, respectively. The effects of the extract on the bacterial respiratory chain dehydrogenase, membrane integrity and permeability were analysed using iodonitrotetrazolium chloride, 260 absorbing materials and relative electrical conductivity assays. GC-MS spectrum revealed 26 compounds with N,N’-trimethyleneurea (10.56%), xanthine (8.57%) and 4-hexyl-1-(7-ethoxycarbonylheptyl)bicyclo[4.4.0]deca-2,5,7-triene (7.10%), being the major components. The extract also exhibited antioxidant activity with median concentration (IC₅₀) values of 0.65 mg/mL on DPPH and 0.052 mg/mL on ABTS. The extract exhibited minimum inhibitory concentration (MIC) values ranging from 0.07 to 1.13 mg/mL. The extract inhibited the bacterial growth by destructing the activity of the respiratory chain dehydrogenase, membrane integrity and permeability. Therefore, the leaf extract has the potential to serve as a source of antibacterial and antioxidant compounds. Full article

Bioflocculation has become the method of choice in wastewater treatment because of its effectiveness, environmental friendliness and innocuousness to humans. In this study, the bioflocculant-producing bacterium was isolated and its bioflocculant was used in wastewater treatment. The isolate was identified by 16S rRNA gene sequencing analysis. Its culture conditions (inoculum size, carbon and nitrogen sources, pH, temperature and time) were optimised using the one-factor-at-a-time assay. The cytotoxicity of the bioflocculant was assessed on human colorectal adenocarcinoma cells (Caco2) by tetrazolium-based colorimetric method. The ability of the bioflocculant to reduce biochemical oxygen demand (BOD) and chemical oxygen demand (COD) in wastewater was evaluated using Jar test. The bacterium was identified as Bacillus subtilis CSM5 and the maximum flocculating activity of 92% was observed when fructose and urea were used as nutrients and the culture conditions were adjusted to 30 °C, pH 9, 160 rpm and 72 h of incubation. Caco2 exhibited 90% viability when the highest bioflocculant concentration of 200 µg/µL was used. The reduction of BOD and COD was achieved at 59 ± 3.1 and 75 ± 0.4%, respectively. In conclusion, B. subtilis CSM5 is a good candidate for bioflocculant production and its bioflocculant has good potential for use in wastewater treatment. Full article

Attention has been paid to bioflocculants production because of their effectiveness, innocuousness and environmental friendliness. This study aimed to characterise a bioflocculant from Bacillus megaterium BMBF and apply it in wastewater treatment. The proteins, carbohydrates and uronic acid were calculated using the Bradford, phenol–sulphuric acid and carbazole assays, respectively. An energy-dispersive X-ray (EDX) and infrared spectrometry were employed for the identification of the elemental composition and effective units, respectively. Cytotoxicity was carried out against Vero (African green monkey kidney) and bovine dermis cells using a colorimetric cytotoxicity assay. The reduction in chemical oxygen demand (COD) and biological oxygen demand (BOD) in domestic and coal mine wastewater was studied using the Jar test. The flocculant was composed of 12% protein, 27% carbohydrates and 61% uronic acid. Infrared spectrometry indicated hydroxyl, carboxyl and amino groups. EDX indicated C (61%) and O (17.5%) as the main elements. The bioflocculant revealed the mean inhibition concentration of 59 ug/mL against bovine dermis and 240 µg/mL on Vero cells. Maximum COD and BOD removal percentages of 97% and 99.3% were recorded on coal mine wastewater treatment and about 99.2% (COD) and 93% (BOD) on domestic wastewater. In conclusion, the bioflocculant from B. megaterium has potential industrial utility. Full article

The application of bioflocculants has become an alternative to that of chemical flocculants in wastewater treatment due to their environmental friendliness and non-toxic effects. This study aimed at isolating a bioflocculant-producing bacterium from marine water, optimisation of its culture conditions, and investigation of the removal efficiency of its bioflocculant on pollutants in wastewater. The bacterium was identified by 16S rRNA gene analysis. Optimal carbon and nitrogen sources, inoculum size, temperature, pH, and time were determined by the one-factor-at-a-time assay. The cytotoxicity of the bioflocculant was assessed on African green monkey kidney and bovine dermis cells using a tetrazolium-based columetric (MTT) method. Its removal efficiencies on chemical oxygen demand (COD), biological oxygen demand (BOD) and sulphur were determined using the Jar test method. The bacterial isolate was identified as Ochrobactrum oryzae AB84113. A maximum flocculating activity of 92% and a yield of 3.768 g/L were obtained when a 1% (v/v) inoculum size was used in the presence of starch and yeast extract at pH 7, 30 °C, and after 72 h of cultivation. The bioflocculant demonstrated non-cytotoxic effects on bovine dermis and African green monkey kidney cells. The bioflocculant removed 98% COD, 91% BOD and 86% of Sulphur. The bioflocculant has potential for pollutant removal from industrial wastewater. Full article

The constant increase in drug resistance, occurrence of incurable diseases and high medical costs, have necessitated bio-prospecting of fungi as alternative sources of therapeutic compounds. This study aimed at assessing the antibacterial effect and mode of action of secondary metabolites from fungal endophyte associated with Aloe ferox Mill. Endophytic fungus was isolated from the gel of A. ferox and identified by internal transcribed spacer (ITS) rRNA gene sequence analysis. The targets of antibacterial activity were assessed based on minimum inhibitory concentration (MIC) and the effect of the extract on respiratory chain dehydrogenase (RCD) and membrane integrity. Fourier transform-infrared spectrophotometer (FTIR) was employed to ascertain functional groups. The fungus with the most promising antibiotic-production was identified as Aspergillus welwitschiae MK450668.1. Its extract exhibited antibacterial activity with the MIC values of 0.5 and 1 mg/mL against Staphylococcus aureus (ATCC 25925) and Escherichia coli (ATCC 25922). It demonstrated the inhibitory effect on the RCD activity and destruction of membrane integrity on the test bacteria. FTIR spectrum revealed hydroxyl, amine and alkene groups. A. welwitschiae MK450668.1 serves as a potential source of effective compounds to combat the challenge of drug resistance. Full article

Bioflocculation is a physicochemical technique often employed to efficiently remove colloidal water pollutants. Consequently, in this study, a bioflocculant was produced, characterised and applied to remove pollutants in mine wastewater. The maximum flocculation activity of 92% was recorded at 30 °C, pH 9.0 when maltose and urea were used as energy sources and 72 h of fermentation at the inoculum size of 1% (v/v). K⁺ proved to be a favourable cation. The bioflocculant yield of 4 g/L was obtained. Scanning electron microscopy illustrated a hexagonal-like structure of the bioflocculant. It is composed of carbohydrates and proteins in mass proportion of 88.6 and 9.5%, respectively. The Fourier transform infrared spectrum revealed the presence of hydroxyl, amide and amino functional groups. More than 73% of the bioflocculant was obtained after exposure to 600 °C using the thermogravimetric analyser. Human embryonic kidney 293 (HEK 293) cells exhibited 95% viability after being treated with 200 µg/µL of the bioflocculant. The flocculation mechanisms were proposed to be as a result of a double layer compression by K⁺, chemical reactions and bridging mechanism. The removal efficiencies of 59, 72, and 75% on biological oxygen demand, chemical oxygen demand and sulphur, were obtained respectively. Thus, the bioflocculant have potential use in wastewater treatment. Full article